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Thy-1.1~+ OT-I CD8 T cells were adoptively transferred into Thy-1.2~+ host C57 mice treated with either isotype or PK136 Ab as mentioned above followed by injection i.v.withΔactA LM-OVA.On day 7 after infection,equal number of Thy-1.1~+ CD8 T effector cells from either hosts were sorted and adoptively transferred into second host mice(Thy-1.2) which were also on day 7 after infection and treated with isotype or PK136 Ab,respectively.

为进一步阐明NK细胞促进CD8Tm细胞的产生是作用于收缩期而不是扩增期,我们设计了如下的实验:将Thy-1.1~+ OT-I CD8 T细胞过继回输到经PK136抗体或对照抗体处理的C57小鼠体内并用ΔactA LM-OVA感染,7天后将两组小鼠脾脏中的Thy-1.1~+ CD8T细胞分离纯化后等量分别回输到同步感染的经PK136抗体或对照抗体处理的第二受体小鼠体内。

We know diseases of stoppings and suffocations are the most dangerous in the body; and it is not much otherwise in the mind; you may take sarza to open the liver, steel to open the spleen, flowers of sulphur for the lungs, castoreum for the brain; but no receipt opens the heart, but a true friend; to whom you may impart griefs, joys, fears, hopes, suspicions, counsels, and whatsoever lies upon the heart to oppress it, in a kind of civil shrift or confession.

你可以服莎直药治肝病,服含铁剂治脾脏,服硫磺治肺,服海狸胶治脑。可是除了挚友以外,没有任何药剂是通心的,因为你可以把你的忧愁、喜悦、恐惧、希望、怀疑、劝告等等传达给忠实的朋友——不管什么压在你心头上的事,如同一种民间的忏悔一样,都可以传达给忠实的朋友。

Methods:①Pharmacodynamics observed the effects of The root and leaf of Panicled Fameflower on swimming time of mouse, and physiological function, IL-2 IgA IgM IgG C3 C4 of rat of asthenia of the spleen, and The leaf of Panicled Fameflower on determinating mininal inhibitory concentration, swelling of mouse ear, capillary vein permeability of mouse skin, granuloma in rat.

①药效学实验:观察土人参根、叶对小鼠游泳时间的影响,以及对&脾虚&大鼠脾脏生理功能,细胞因子IL-2、IgA、IgM、IgG、C3、C4的影响,验证土人参根、叶&健脾&功效;观察土人参叶体外抗菌实验,对二甲苯致小鼠耳廓肿胀的影响,对小鼠皮肤毛细血管通透性增高的影响,以及对大鼠棉球肉芽肿的影响,验证土人参叶&解毒消痈&功效。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法,首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后,其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量;中枢免疫器官胸腺和法氏囊,外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

Surgical procedures included eight proximal pancreatectomies, three total pancreatectomies, nine distal pancreatectomies, and three tumor enucleations from the pancreatic head. Three patients had superior mesenteric vein reconstruction, six had liver resection, one had an extended periaortic node dissection, 11 had splenectomy, and one had left nephrectomy.

手术的过程包括8个近端的胰脏切除,三个全胰脏切除,九个末梢的胰脏切除,和三个进行肿瘤切除,三个患者有较佳的中肠静脉重建术,六名进行肝脏再切除,一名患者有广泛的进大主动脉节切除,1人进行脾脏切除,一名患者进行左肾切除术。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算,连续10d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算,连续10 d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

By comparing the body weight, immune organs index and response of lymphocytes to ConA, to analyze the effect of QKL on immunological system of normal mouse. Delayed type hypersensitivity mouse model was established by stimulation with dinitrofluorobenzene. By comparing spleen index and swelling rate of pathogenetic ear, to illuminate the immunoregulation of QKL in vivo. Polymicrobial sepsis mouse model was induced by cecal ligation and puncture. The cumulative survival rate was observed in QKL and NS groups. Results: QKL had no influence on body weight and spleen index of normal mouse, but it could significantly decrease thymus index and inhibit the immune response of lymphocytes to ConA.

连续皮下注射QKL 5 d,通过检测小鼠体重、免疫器官指数和淋巴细胞对ConA刺激的反应,分析QKL对正常小鼠免疫功能的影响;采用二硝基氟苯诱发小鼠耳朵肿胀,发生接触性皮炎,建立DTH模型,通过比较QKL与NS对照对小鼠脾脏指数和发病耳肿胀抑制率评价QKL体内对小鼠的免疫调节功能;采用盲肠结扎穿孔术后引起的多细菌性腹膜炎,建立小鼠脓毒症模型,通过观察QKL对小鼠存活状况的影响,评价QKL的免疫调节作用。

This is particularly relevant to closely monitored conservative management of limited splenic injury.

这与严密有限的脾脏伤害的被监测的保守的管理是特别相关的。

Even making a mouse hybridoma is tricky: B cells from the spleen of an immunized mouse, which normally died out in culture dishes, must be fused with cancerous white blood cells called myelomas.

制造小鼠的杂交瘤还有一个很难解决的问题:从免疫过的细胞脾脏中取到的B细胞,它在培养器皿中会逐渐死亡,如果一种叫骨髓瘤的白血细胞就保险得多。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

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