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General symptoms such as purpure in mucous membrance and skin, bleeding of internal organs, decreasing of weight and bad mental state caused by modeling were well improved in four control groups (Chinese traditional medicine group, western medicine group, PG original formula group and PG major formula group), the peripheral PLT was increased, spleen weight and its action of phagocytosis and damage to PLT were both decreased, morrow megakaryocytes differentiation and maturation were improved and hyperplasia descended, which showed significant differences compared with the model group. Differences among the four control groups were not significant. The optimal dose ratio of PG major formula was finded out with homogeneous design software UST3.0 and multiple regression: Astragalus root 48g, Rhubarb 15g, peach kernel 1Og, Agrimony 24g.

结果:模型建立成功;中、西药对照组、紫癜颗粒核心方组与原方组小鼠对于免疫法造模所引起的皮肤粘膜紫癜、内脏出血、体重减轻、精神状态差等一般症状均有较好的改善;小鼠的外周血小板计数得到提高;脾脏均重及对血小板的吞噬破坏降低;骨髓巨核细胞分化成熟程度增高,增生活跃度降低,与模型组相比较,均有显著性差异,四个对照组之间无显著性差异,利用均匀设计UST3.0统计软件分析,进行多元逐步回归处理,筛选出紫癜颗粒核心方的最佳剂量配比为黄芪48g,大黄15g,桃仁10g,仙鹤草24g。

Results Being compared with the normal group, the PDS mice showed a slower growth of body weight and a lower body temperature, a shorter mean retention time of chyme in digestive tract and faster gastric emptying velocity, a lower gastrin content, and a higher motilin content. Also splenic and thymus indexes were found to follow a downtrend in the PDS mice. Three dosages of SJZD showed a restoration effect on the above all indexes, and the high dosage was the most effective one, especially.

结果脾虚证小鼠体重增长缓慢,体温降低、消化道内食糜平均滞留时间缩短、胃排空加快、胃泌素的量降低、胃动素的量升高,脾脏和胸腺指数降低。3种剂量的四君子汤对脾虚证小鼠以上各指标的变化均有明显的矫正作用,以高剂量效果最佳。

The studies on the capacities of immune responses of lymphocytes in peripheral blood and immune organs of Grass Carp (Ctenpharyngoden idellus Cuvier et Valencinnes) to PHA and SPA with incorporation of 〓H-TdR and active reaction of Acid α-Naphthyl Acetate Esterase indicated that there were T and B lymphocytes in peripheral blood of grass carp, but only T lymphocyte and no B lymphocyte in the thymus of grass carp. It was justified that the T lymphocytes in peripheral blood was originated from the thymus, and B lymphocyte could be from the spleen and anterior kidney of grass carp.

运用〓H-TdR掺入法和酸性醋酸萘酯酶活性检测法分别研究了草鱼外周血以及免疫器官中的淋巴细胞对PHA和SPA的免疫应答以及化学标志特征,发现草鱼外周血中存在着类似于哺乳动物的T、B两类不同的淋巴细胞亚群;同时也发现胸腺中只有T淋巴细胞,而无B淋巴细胞,提示了胸腺中淋巴细胞可能是外周血中T细胞的来源,而B淋巴细胞可能来自脾脏或前肾。

Result] Under blood stasis, it can to some extent inhibit the production of primary cancer, the serum IL18 and HGF were reduced in mouse of liver metastasis with cancer, which may intervene the liver metastasis; Shensanqi extract had some definite function on the primary cancer of spleen and liver metastasis of rats with liver metastasis of cancer, the platelet exerted key functions in cancer hyperplasia, infiltration and metastasis.

结果]在血瘀状态下,可在一定程度上抑制原发肿瘤的生长,荷瘤肝转移小鼠血清IL18和HGF的水平降低,可能干预了肝转移的过程,参三七醇提液对荷瘤肝转移大鼠的脾脏原发肿瘤的生长和肝转移有一定抑制作用,血小板在肿瘤增殖、浸润和转移中起着重要作用。

The luminescence intensity of suspended immune cells (1×10〓 cells/ml) from peripheral blood, anterior kidney and spleen of grass carp, which were from two groups immunized with being injected Aeromonas hydrophilia and unimmunized respectively, were detected by means of BPCL4 Intelligentized Ultraweak Luminometer, the datum that were dealt with statistics demonstrated that luminescence intensity of the cells from all kinds of immune organs and blood were intensified after the fish were immunized in two weeks and the luminescence intensity from anterior kidney cells was the highest, mitogens had the enhancing effects. Luminol could strengthened luminescence intensity.

把未免疫的和经过嗜水产气单胞菌注射免疫的草鱼外周血、前肾、胸腺和脾脏以及它们的悬浮细胞(密度控制在1×10〓个细胞/ml左右)分别用BPCL4智能化微弱发光测量仪测量各组的发光强度,结果显示:草鱼各类免疫器官与细胞在免疫两周后发光强度增加,肾细胞的发光强度最大;有丝分裂原具有增强作用;化合物鲁米诺能提高发光强度。

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性,进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠,3次免疫小鼠后,血清(1:1000)中能检测到较强的MUC1抗体阳性;以最大OD450值之纯化血清做为一抗行蛋白印迹分析,结果显示:纯化血清可识别MUC1蛋白;小鼠脾脏淋巴细胞增殖率检测结果表明:噬菌体展示肽表位可在无佐剂存在情况下,大大促进小鼠的脾淋巴细胞的增殖,证明噬菌体是一较好的生物载体。

Methods: Mice bearing in vivo transplanted tumor-H22 hepatic carcinoma were used to evaluate the drug"s activity of anti-tumor and GAPSGF"s efficacy enhancing effect by calculating the ratio of antitumor and q. HE dyeing was applied to observe the tumor"s morphological changes after the anti - tumor drug. It is observed the change of theperipheral white blood cells in serum and the body weight to analyze the GAPSGF"s toxicity reducing effect on the anti - tumor drug-Cytoxan. Applying ELISA method to observe the change of IL-2 in serum ,Applying carbon clearance method to test the function of uninuclear phagocyte,took the weight of the spleen and thoracic gland, analyzed the mechanism — efficacy enhancing and toxicity reducing of GAPSGF.

构建体内动物移植性H_(22)肝癌模型,通过计算抑瘤率与q值来分析药物的体内抗肿瘤活性,判断树舌多糖GF注射液的增效作用;采用瘤组织常规HE染色观察药物作用下的肿瘤细胞形态变化;观察外周血WBC数和体重改变来分析树舌多糖GF注射液对环磷酰胺抗肿瘤的减毒作用;采用ELISA法测定血清IL-2、小鼠碳粒廓清法测定单核吞噬细胞功能、取胸腺与脾脏称重测定脏器指数等以分析树舌多糖GF注射液增效减毒的作用机制。

Immunohistochemical method was used to detect avian leukosis virus subgroup J from egg-type chicken, which was preliminarily diagnosed to appear subgroup J avian leukosis. The organs from these chickens were examined by ALV-J gp85 monoclonal antibody, these organs included marrow, liver, spleen, kidney, lung, heart, pancreas, oviduct, ovary, ventriculus glanduaris, skeletal muscle, cerebrum, ischiacticus nerve.

采用免疫组化法,对病理学初步诊断为蛋用型鸡 J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经,用特异性抗 J亚群禽白血病病毒囊膜糖蛋白gp85的单克隆抗体进行检测,待检的组织切片中均检出阳性抗原,免疫组化的研究结果与病理学诊断结果相一致。

Conditions placing persons at prolonged increased risk for meningococcal disease include persistent complement component deficiencies (such as C3, properdin, Factor D, and late complement component deficiencies), anatomic or functional asplenia, and prolonged exposure (eg, microbiologists routinely working with Neisseria meningitidis, or travelers to or residents of countries where meningococcal disease is hyperendemic or epidemic).

使人们持续处於感染脑膜炎球菌疾病高风险的因子,包括持续的补体缺乏(例如C3、备解素、D因子、以及后期补体缺乏),解剖或是功能性脾脏功能丧失、以及延长暴露(例如经常处理奈氏脑膜炎球菌的微生物学家、或是到脑膜炎球菌大流行或流行国家旅行或居住的人们)。

Contrast-enhanced ultrasound has been widely used in the imaging of liver and other organs such as kidney, pancreases, spleen, prostate, ovarian, uterus as well as abdominal trauma, showing promising values in the diagnosis and differential diagnosis of various diseases.

近年发展起来的微泡超声造影剂已经在临床中得以应用,超声造影也已广泛用于肝脏及肝脏以外器官的显像,如肾脏、胰腺、脾脏、前列腺、卵巢、子宫和腹部外伤,并在多种疾病的诊断和鉴别诊断中显示出日益增长的潜力。

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