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Objective To discuss the feasibility of amplification cultivation of the mice spleen dendritic cells in vitro.

目的 建立简便的体外扩增小鼠脾脏树突状细胞的方法。

It is concluded that the apoptosis of spleen and bursa cells was obviously affected by heat stress and regulated by the expression of caspase-3 and Bcl-2 protein.

据此说明高温应激能明显影响脾脏和法氏囊细胞凋亡,而这种细胞凋亡的变化受Caspase-3和Bcl-2表达的调节。

Type Ⅱ collagen-induced arthritis in mice,which shares similar characteristics with rheumatoid arthritis,was used as the mode for RA in this study,by taking the incidence,arthritis index,the level of anti-collagenase Ⅱ antibody,the histo-pathological changes as quotas,we observed cantharis plaster's effect on the model by applicating it on acupoints,and measured the splenic interleukin-1 β level of collagen-immunized mice.

采用与类风湿性关节炎特征相似的小鼠胶原诱导性关节炎(Collagen-induced arthritis,CIA)模型,以关节炎发病率、关节炎指数、抗Ⅱ型胶原抗体水平、组织病理学改变等为指标,观察了斑蝥膏穴位敷贴对此模型的影响,并检测了胶原免疫小鼠脾脏白介素-1β水平。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法获取人脾脏组织yrdC基因序列,采用AdEasy腺病毒载体系统构建携带yrdC基因的高滴度重组腺病毒Ad-yrdC,将其转染胃癌BGC-823细胞,应用Western blot和免疫组织化学法检测yrdC蛋白表达,设对照组和转染Ad-Null组,并分别绘制细胞生长曲线和MTT比色法研究Ad-yrdC对BGC-823细胞的影响。

He had been crossing to the store cupboard to get more rat spleen when he felt, rather than saw, the heat from the potions explosion; he was aware of a chartreuse mist lingering in the air, and heard a muttered spell from Professor Snape.

正当他走向储藏柜去取一些大鼠脾脏时,与其说是看到了,不如说是感觉到了魔药爆炸的高温;他意识到空中弥漫着一股黄绿色的雾气,听到斯内普教授低语了一声。

It's good for people's spleen, but detrimental to people's teeth.

对人的脾脏有好处,但对人的牙齿有坏处

According to the gallus colony stimulating factor(colony-stimulating factor,CSF)cDNA sequences published by GenBank,a pairs of specific primer was designed,the local dorking was injected 100 μg/mL ConA,total RNA was extracted from feeding 24 hours lymphocyte from spleen,then dorking colony stimulating factorcDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor,CSF)cDNA序列,利用Primer Premier5.0软件设计一对特异性引物,对本地肉鸡注射100μg/mL ConA试剂,饲养24 h后,取脾脏提取淋巴细胞总RNA,利用RT-PCR技术克隆集落刺激因子的cDNA片段。

According to the gallus colony stimulating factor (colony-stimulating factor, CSF) cDNA sequences published by GenBank, a pairs of specific primer was designed, the local dorking was injected 100 μg/mL ConA, total RNA was extracted from feeding 24 hours lymphocyte from spleen, then dorking colony stimulating factor cDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor, CSF)cDNA序列,利用Primer Premier5.0软件设计一对特异性引物,对本地肉鸡注射100μg/mL ConA试剂,饲养24 h后,取脾脏提取淋巴细胞总RNA,利用RT-PCR技术克隆集落刺激因子的cDNA片段。

They've shown that the spleen is what they call a critical reservoir of monocytes.

他们已经显示了脾脏是一个被他们称为&单核白细胞的存储库&。

They've shown that the spleen is what they call a critical reservoir of monocytes.

研究发现,脾脏是他们称之为的重要单核细胞库。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。