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Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.

H.E。染色观察显示,病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等;淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少;有阳性反应的细胞,特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。

The effect of oral infection and subsequent challenge with Eimeria vaccines on the populations of CD4 , CD8 T lymphocytes in the intestine, cecal tonsils and spleen of chickens was assessed by immunohistochemistry.

用免疫组化ABC法检测鸡用上述球虫进行第一、二次免疫后小肠或盲肠、盲肠扁桃体和脾脏中CD4~、CD8~ T淋巴细胞的动态变化。

The immuneregulating action that briefed on the electropuncture further regulated each other throughthe neural peptide / neurotransmitter, hormone and CK. 6Function laws, which compare point: Dazhui is good at suppressing theinflammation, while Mingmen is strong to the regulating immune function; it is Feixuethat has regulating action too.

比较腧穴的免疫调节作用规律:大椎穴的抗炎治疗作用(如足爪肿胀率、下丘脑 CRH 含量,血清 TNFα含量)较强,命门穴对于免疫调节作用(如脾脏 T、B 淋巴细胞转化率、IL-2 活性、肾上腺 IL-2 含量等)较强,非穴也具有一定的抗炎免疫作用,但弱于大椎穴及命门穴。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果:1、通过不同感染途径的实验证实,滴鼻感染的小鼠主要表现为间质性肺炎,而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现,随着感染Q热立克次体剂量的加大,动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现,在腹腔注射后第2d的脾和肝脏即可发现病原体,主要脏器的病理变化从第2d到第12d逐渐加重,第7d动物的病变最典型,至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统,在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内,呈多形性,多见圆形和杆状,大小不一。

The change of general physiological characters, D-xylose excretion, activity of amylase in serum, stomach emptying and enterokinesia speed, contractile activity of small intestine in vitro, morphological change of gastrointestinal mucosa, gastrin content in serum, spleen index, thymus index were observed.

实验主要观察了动物的一般体征和消化吸收功能的变化,测定的指标有:D—木糖排泄率、血清淀粉酶活性、胃排空速率、肠推进速率、离体小肠张力、胃肠粘膜形态、血清胃泌素含量、脾脏指数和胸腺指数。

Results Systolic pressure,pulse rate,blood loss,whole abdominal muscular tension,enteroparalysis,spleen traumatic division of USA and two or more than two other damaged organs were the factors affecting the prognosis of spleen rupture.

结果 收缩压、脉率、出血量、全腹肌紧张、肠麻痹、脾脏损伤级别、合并2 以上其他脏器损伤是影响脾破裂预后的因素。

Results Systolic pressure,pulse rate,blood loss,whole abdominal muscular tension,enteroparalysis,spleen traumatic division of USA and two or more than two other damaged organs were the factors affecting the prognosis of spleen rupture.

结果 收缩压、脉率、出血量、全腹肌紧张、肠麻痹、脾脏损伤级别、合并2个以上其他脏器损伤是影响脾破裂预后的因素。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组,连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数;采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响;血清溶血素实验测定对小鼠体液免疫功能的影响;ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响;MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

Methods Study the effect of G. lucidum spores oil (at the dose of 2.5,1.25,0.725 g/kg) on reducing the toxicity of the Cy chemotherapy and 60Co radiotherapy by detecting the number of the peripheral blood leucocyte, the number of bone marrow karyota;spleen index, thymus index of normal mice and H22-bearing mice by carbon clearance test and serum erythrocytolysin test. Results It showed that G.

方法以正常和 H22荷瘤小鼠为对象,通过检测外周血 WBC数、骨髓有核细胞数、脾脏指数、胸腺指数来观察口服灵芝孢子油(2.5,1.25,0.625 g/kg)对于环磷酰胺化疗及60Co放疗的减毒作用;通过小鼠碳廓清实验、血清溶血素测定实验检测药物对 H22荷瘤小鼠免疫功能的影响。

The results showed that the canine models were established successfully with stable thrombocytopenia, erythropenia , hypochromia, heightening of the pressure of splenic vein and splenomegaly .

结果表明:该手术方法可以建立稳定的血小板、红细胞和血红蛋白降低,脾脏体积增大,脾静脉压增高的继发性脾功能亢进动物模型。

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