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Histochemical, immunochemical and in situ hybridizative methods and technique of computer image analysis were used in this study to investigate the variable trends of expression and correlation of some proteins and genes interrelated with abnormal proliferation of hepatocyte during periods of hepatocarcinogenesis induced by diethylnitrosamine, and the effect of Ganfujian Granule, which is composed of drug-food double-duty herbs, taking"jian-pi-shu-gan"(invagorating spleen and soothing liver) as therapeutic principle and manufactured by our department. The purpose of the study is to screen out an applicable and effective liver cancer preventive agent of traditional Chinese medicine, to offer academic foundation for further clinic study and to spread out its application.

本课题通过组织化学、免疫组化、原位杂交等方法及计算机图像分析等技术，检测了化学致癌物二乙基亚硝胺诱导大鼠肝癌形成过程中与肝细胞异常增殖相关的部分蛋白、基因表达的动态变化和相互关系及我科研制的以"健脾疏肝"立法的药食两用中药制剂肝复健冲剂对诱发肝癌各时期上述因素异常变化的影响，旨在筛选适用、有效的中药肝癌预防制剂，并为进一步的临床试验及推广应用提供一定的理论依据。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

Immune cells were separated from blood and head kidney of silver carp and immunestained with fluorescein-labbled LPS and mouse-anti LPS antiboby.

对银鲫接种从鱼类致病菌中提取的脂多糖后，观察了LPS在鱼体内的分布，获得了进入鱼体内的LPS主要是分布在鱼体的肾、脾和肝等免疫组织中、而且在肾脏中消失的最慢的结果。

Mice immunized with DeltaF co-formulated with CpG ODN, indolicidin, and polyphosphazene (DeltaF/CpG/indol/PP) developed higher levels of DeltaF-specific serum IgG, IgG1, and IgG2a antibodies when compared to DeltaF alone, and displayed an increase in the frequency of IFN-gamma-secreting cells and decreased IL-5 production by in vitro re-stimulated splenocytes, characteristic of a Th1 immune response.

用DeltaF与CpG ODN， indolicidin，聚磷腈配合使用来免疫的小鼠与单独使用DeltaF相比，产生了更高水平的DeltaF特异性免疫血清 IgG， IgG1，和 IgG2a抗体，并表现出伽马干扰素分泌细胞频率的增加，以及通过体外重新激活的脾细胞降低的IL-5产量，这些都是Th1型免疫应答的性质。

The two can cause ultrastructural pathogenesis widely in many organs and tissues such as hepar, pancreas, lien, ren, pulmo, cerebral, cerebellum, cor, muscular and so on, of them ,caused by i~olates YG97 and Y98 more obvious in epithelial cells of digestive tract and unobvious in epithelial cells of respiratory tract ,but ultrastructural pathogenesis caused by NDV strain F48E8 ,more obvious and more serious in glandularis and muscularis ventriaculi and in epithelial cells of respiratory tract .

将鹅源分离株YG_(97)、鸡源分离株Y_(98)和NDV强毒株F_(48)E_8感染SPF鸡，运用扫描电镜和透射电镜观察该二株病毒对机体细胞的影响，结果显示三株禽副粘病毒均引起机体肝、胰、脾、肾、肺、小脑、大脑、心和肌肉等实质器官组织细胞的超微病变，其中YG_(97)和Y_(98)引起消化道粘膜上皮细胞发生微绒毛脱落、纤毛脱落、分泌颗粒增多、细胞游离面破溃等显著的超微病变，引起气管粘膜上皮细胞的不显著超微病变；F_(48)E_8强毒株引起胃粘膜显著而严重的超微病变；还引起气管粘膜上皮严重的超微病变。

Bian Zheng Shi stressed that the Chinese food, for example, the patient's tongue is greasy, yellow greasy or Baini, you should eat spleen Huashi of food, such as: MI Ren, lentils, etc., but definitely can not eat soft-shelled turtle, so and implement the patient's diet should be based on the specific circumstances.

而中医强调辨症施食，比如说，病人的舌苔是腻的，黄腻或白腻，就应该吃健脾化湿的食物，比如：米仁、扁豆等，而甲鱼肯定是不能吃的，因此，实行食疗应根据病人的具体情况而定。

Based on above results, for well- appearing elderly advanced non-small cell lung cancer patients(KPS≥60), it is reasonable to treat them with single agent chemotherapy and traditional Chinese medicine which have the effects of supplementing Qi and nourishing yin, promoting blood circulation and removing blood stasis, invigorating the spleen and tonoifying the lung. As for poor- appearing patients (KPS0), traditional Chinese Medicine and symptoms release and supportive therapy should be the main choice.

因此，对于一般情况较好（KPS≥60分）的高龄晚期非小细胞肺癌患者在单药化疗的同时，配合使用益气养阴、活血化瘀、健脾益肺的中药治疗是一种较为合理的治疗途径，而对于一般情况较差（KPS0分）的患者则应以中药治疗加对症支持治疗为主。

Yi TAI Compounded Medication "we used bases on the basic theory of TCM,set up "The treatment of invigoratmg spleen and promoting blood by regulating qi",target defiency in origin and excess in superficiality TCM syndrome nature,and take experiments;our research injecting L-Arg into abdomen to induce Spleeh-asthenis rat model,it is a new model which combine diseases and syndromes;immunohistochemic 、 optical mirror and biochemistry value number index number are used to detect the expression of related constrain factor TGF-β_1 mRNA、α-SMA when textnre fabrosis occurs in pancreatic,as nell as significance and relation of hydroxyproline in the process of pancreatic fibrosis,meanwhile observe the disturbace which "Yi TAI Compounded Medication makes,revel the anti-fibrosis mechanism from organization form and molecular level.

本研究采用L—精氨酸分次腹腔注射诱导脾虚型慢性胰腺炎大鼠模型，是一种病证结合的新模型；用免疫组化、光镜等技术和生化指标检测CP时胰实质纤维化的相关调控因素TGF-β_1、α-SMA的表达和血清中羟脯氨酸在胰腺纤维化过程中的作用及相互关系，同时观察了&胰泰复方&对其干预机理，从组织形态学和分子水平揭示了该方抗纤维化的作用机制。

The author point out that skeletal muscle conforms to the physiological features of the liver and therefore liver stored in the body is skeletal muscle in the modern sense, thus perfecting the theory of the relationship between liver and the smooth muscle.

从肝藏的生理解剖基础是人体平滑肌系统这一认识出发，分析了&筋&与&肌肉&的概念，论证了平滑肌与骨骼肌的客观联系，指出骨骼肌与肝藏的生理特性相符，肝藏在体所合是现代意义上的骨骼肌，脾藏在体所合应为脂肪。

The ground of anatomy was laid by Vesalius in the 16th century (1543). Subsequently, Harvey discovered blood circulation in 1616. The invention of microscope enabled people to extend the observation further. Malpighi discovered capillaries (1616), bridging the gap between artery and vein. Meantime, the microscopic structures of lungs, spleen, kidneys, liver, skin and many other organs were revealed gradually. This is an excellent example how a technical breakthrough can contribute to the advancement of biomedical science.

自从 Vesalius 在 16 世纪(1543)奠定了人体解剖学的基础而 Harvey 在 17 世纪中叶(1616)宣布血液大体循环的发现之后，由於显微镜的发明，使得 Malpighi 能在 17 世纪中叶(1616)发现了微血管，将大体循环的动脉与静脉两系统联结起来，同时也对肺、脾、肾、肝、及皮肤的显微构造开始有了概念，这可以说是科技对医学进步影响最早的范例。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失