脾的

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

ABSTRACT: Objective In order to investigate the therapeutic effects of portal arterialization for portal hypertension, portal arterialization and complete shunt was applied in canine model of portal hypertension, which was made by thread embolization within the portal vein.

目的 为评估门静脉脾动脉化对门脉高压症的治疗效果，对丝线栓塞性门脉高压模型犬进行脾动脉门静脉吻合联合完全门体分流，并观察治疗效果。

Methods The hepatic cirrhosis induced portal hypertension patients with Child A or B grade of liver function were randomly divided into splenic autotransplantation group and splenectomy group.

将20例肝功能Child A、B级的肝硬变门静脉高压症患者随机均分为自体脾移植组和切脾组。

And There was no serious complication;②In the course ofinterventional therapy, Direct portal vein angiography demonstrated vena coronaria ventriculi(100%)andgastricveins(65.26%)andvenagastricaposterior38.43%, Angiography demonstrated venacoronariaventriculi communicate esophagus varicose veins, gastric veins and vena gastrica posterior communicategastric varicose veins. vena coronaria ventriculi had only a small percentage of double vein, about30.57%. The sites of vena coronaria ventriculi arising from the portal vein, splenic vein, portosplenic junction, were found in 52.06%、27.39%、20.55% respectively.③12 extrahepaticprotosystemic shunts were found in these patients. Include gastro-nephrosshuntof 3 cases, 7 caseswere splenetic- nephros shunt and 2 cases shown recanalization of umbilical vein .④The averageportal pressure before and after the procedure were 3.87±1.82kPa and 3.64±1.14kPa in 73patients, but to the time of rebleeding, it was 3.96±0.23kPa in the 11 cases.⑤There werethree kinds of variceal outcome: disappearance (54,low degree (19).⑥Spearman logisticanalyse and ANOVAtest shown liver function class, variceal degree of the splenic necrosis area,the blood direction in portal vein before operation and remain small collateral routes were thesignificant factors concerning outcome of varices.⑦The bleeding volume and portalhypertensive gastropathy are main risk factors of rebleeding.⑧The course of livercirrhosis is the risk factor of survival and extrahepatic portosystemic shunt , fine varices are thebeneficial factors to survival.⑨During all cases'followed-up data, the 1, 2, 3, 4, 5 yearcumulative survival rates and rebleeding rates were 17.81%, 28.77%, 38.36%, 43.84%, 47.95%and93.15%,91.78%,86.30%,83.56%,80.82%respectively. Conclusion The interventional disconnection treatment for liver cirrhosis and portalhypertension was designed suitability. It rapidlycontrol bleeding,butpressure of portal vein was notobvious high, perfusion was not low .it was compared with surgery therapeutic that interventionaldisconnection treatment was safe and had a significant clinical effect to hemorrhage and preventfrom rebleeding.

结果：①术后一过性发热62例(84.9%)，腹痛腹胀48例(65.8%)是介入断流术常见的并发症，未发生严重并发症；②门静脉造影显示胃冠状静脉、胃短静脉和胃后静脉的曲张分流的出现率是100%、65.26%和38.34%；显示食管静脉曲张主要由胃冠状静脉供血，胃静脉曲张主要由胃短静脉和胃后静脉供血；胃冠状静脉大多数为单支，少数为双支，其双支的出现率分别为30.57%；胃冠状静脉开口于门静脉主干的为52.06%，开口于脾静脉主干的为27.39%和开口于门脾静脉交汇处的为20.55%；③发现胃肾分流3例，脾肾分流7例、腹膜后门腔静脉分流2例，以及CTA检查发现脐静脉开放者2例；④73例患者介入断流术前和术后平均自由门静脉压力分别为3.87±1.82kpa和3.64±1.14kpa，前后比较存在显著性差异；11例再次介入手术患者的术前、术后和复发后的自由门静脉压力分别为4.02±0.24kpa、3.82±0.25kpa和3.93±0.23kpa ，前后比较发现首次术前与术后存在显著性差异，首次术前和复发出血术前门静脉压力比较无显著性差异；⑤介入术后复查曲张静脉转归基本消失54例，轻度19例；⑥Spearman相关分析和Logistic多因素回归分析，肝功能分级、静脉曲张程度、门脉血流方向和残存小侧支四个因素对曲张静脉转归有影响；Spearman相关分析和Logistic多因素回归分析门脉高压性胃病和出血量等因素对复发出血时间有影响；⑦COX回归分析，门体分流和曲张静脉转归两个因素对术后生存有影响；⑧术后随访6-70月，1、2、3、4、5年的累计复发出血率和累计生存率分别为17.81%、28.77%、38.36%、43.84%、47.95%和93.15%、91.78%、86.30%、83.56%、80.82%；结论：介入断流术治疗门脉高压食管胃底静脉曲张有独特的优点，可以快速直接控制曲张静脉出血而门静脉压力无显著增高，保证了肝脏灌注；与外科分流术相比适应证广、损伤轻、术后恢复快，不易遗漏曲张静脉；肝功能分级、曲张静脉程度、门脉血流方向和残存侧支血管对食管胃曲张静脉转归有影响；门脉高压性胃病对复发出血时间有影响；门体分流和曲张静脉转归对生存时间有影响。

The careful evaluation before operation, standardized operative procedure, correct skillful technics of the dissection for perisplenic adhesion and handling of splenic pedicle are the crucial factors determining the safety of complex splenectomy.

完善的术前评估，规范的手术操作，正确的脾周粘连的解剖分离及脾蒂的处理，是确保复杂性脾切除安全的关键。

Passive transfer of sera from mice immunized with expressed G1 and G2 conferred partial protection or prolongation of time to death from fatal hantaan virus infection in infant mice which challenged with hantaan virus at 10LD50. The spleen cells from mice immunized with G1, G2and NP can protect or prolongate the lifetime of infant mice infected with lethal dose (10LD50 or 40LD50) hantaan virus.

将重组G1、G2和NP免疫小鼠的血清和脾细胞注入汉滩病毒致死性感染的乳小鼠体内，发现G1、G2的免疫血清和G1、G2及NP免疫脾细胞对乳鼠有保护作用，同时在一组试验中观察到G1的脾细胞可显著缩短乳鼠的存活时间。

In the experimental study section, the rats were divided into four groups, including the normal control, the model, the Piracetam, the QPC, and copied to AD model. After the treatment, we observed the effects on the AD rats' ability of leaning and memorizing, detecting the TChE activity in whole blood and the expression levels of the related biochemical indicators of As includeing GFAP、NGF and BDNF of the AD rats' brain hippocampus, then compared the QPC with other groups to reveal the mechanism of action of the QPC treating AD to bright.

在动物实验部分，我们通过塑造AD动物模型，设立正常对照组、模型组、脑复康组和强脾益智胶囊组，观察强脾益智胶囊对AD模型大鼠空间学习记忆能力的影响，检测AD大鼠全血、海马匀浆液中乙酰胆碱酯酶活性和通过免疫组化的方法检测胶质纤维酸性蛋白、神经生长因子、和脑源性神经营养因子的表达情况来反映星形胶质细胞的增值情况，以期揭示强脾益智胶囊对AD的治疗作用机理。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中，Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性克隆分析显示，在睾丸组织中表达的是TSSox1和TSSox4基因，而在脑组织中表达的是TSSox2和TSSox4基因，脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用，还可能在神经系统、免疫系统多种组织中起重要作用

Using method explained in this article can establish stable model of Spleen Deficiency and gastric carcinoma and metamouse model of gastric carcinoma and Spleen Deficiency,which all combine with disease and syndrome;proved that Spleen Deficiency mainly promotes occurrence and development of gastric carcinoma,which is realized by disordering correlative gene expression of gastric carcinoma.

用本文所述方法可复制出稳定的病证结合的脾虚胃癌动物模型、脾虚胃癌转移鼠动物模型；从实验的角度证明了脾虚在胃癌的发生、发展过程中有着重要作用，并提示这种作用可能是通过影响一系列的胃癌相关基因的异常表达完成的。

In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7%of endogenous murine α-globin gene.

5%。在2只长期造血重建小鼠的骨髓、脾、胸腺及另一只小鼠的骨髓及脾中整合有人β-珠蛋白基因，在一只重建小鼠的骨髓和脾中有人β-珠蛋白基因表达，约为鼠α-珠蛋白基因的7 ％。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失