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The immunosuppressive effect was determined with ratio of lymphocyte blastogenesis,phagocytotic function of macrophage,percentage of peripheral T-lymphocyte,delayed type hypersensitivity and antibody forming cell assay.

免疫抑制作用:测定小鼠脾淋巴细胞转化率;巨噬细胞的吞噬功能;外周血T淋巴细胞的百分率;迟发性的变态反应;血清溶血素和抗体形成细胞功能测定。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the activity

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Results Sheep spleen polypeptides could significantly stimulate multiplication and transformation of mice's spleen lymphocytes; increased markedly the weight of spleen and the thymus; promoted delayed hypersensitivity; increased the quantity of antibody-producing cells; increased the level of serum hemolysin; promoted the expurgation effect and the phagocytic function of monocyte/macrophage and increased the activity of natural killer.

结果 羊脾多肽能使小鼠脾、胸腺增重,明显刺激小鼠的脾淋巴细胞增值,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,但促进小鼠单核-巨噬细胞碳廓清作用不明显,增强小鼠的单核-腹腔巨噬细胞吞噬能力,有效提高小鼠自然杀伤细胞活性。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the acˉtivity of natural killer.

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组,连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数;采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响;血清溶血素实验测定对小鼠体液免疫功能的影响;ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响;MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

The acute toxicity andmain organs of pathological section for 30 days were observed. Result 1. Ganoderma lucidum spores oil at 0.20g/kg, 0.40g/kg, 1.20g/kg dose couldsignificantly promote the lymphocyte transformation, raise clearance index numberand phagocytic index number, enhance the activity of NK cells and the level of TNF-α, IL-2 in spleen. Ganoderma lucidum spores oil at high dose and middle dose couldsignificantly enhance the serum erythrocytolysin level.

实验结果 1低、中、高灵芝孢子油组(0.20g/kg,0.40g/kg,1.20g/kg)均可提高小鼠单核-巨噬细胞的吞噬指数及碳廓清指数,促进ConA诱发脾淋巴细胞转化,增强小鼠NK细胞活性,提高脾细胞产生TNF-α和IL-2的能力;高、中剂量灵芝孢子油可提高小鼠的血清溶血素水平。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算,连续10d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算,连续10 d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

Rays,the humoral cellular and non-specific immunity in mice were all decreased. Genistein could facilitate recover all the above indices in the following aspect: lymphocyte stimulation index, delated hypersensitivity, production of antibodies, colony forming units- spleen, and spleen index.

4Gen可明显增加辐射小鼠脾淋巴细胞增殖指数、二硝基氟苯诱发耳廓肿胀率、血清溶血素水平、脾指数,使碳粒廓清指数、胸腺指数比对照组有增加的趋势。

METHODS:Observe the effect of SKR on immunity function in mice,the phagocytotic rate and phagocytotic index,haemolysin in serum,plaque forming cell,and the proliferation of splenocyte following administration SKR for 15 days.

以SK初乳素R-1连续灌胃给药15 d,测定小鼠增强小鼠炭末廓清能力、小鼠血清溶血素抗体水平、血清IL-2含量、抗体生成细胞数、脾淋巴细胞增殖能力等。结果:SK初乳素R-1各剂量组能增强小鼠炭末廓清率和廓清指数,各剂量组均能明显升高小鼠血清溶血素抗体水平(P.01),提高小鼠血清IL-2含量(P.01),促进小鼠抗体细胞形成(P.01),增强ConA诱导的脾淋巴细胞增殖能力(P.01)。

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