脲酶

In order to know the effects of mulch-drip irrigation on soil enzyme activities of saline-sodic soil with shallow water table, activities of alkaline phosphatase, urease, and invertase around the dripper, within 30 cm in horizontal direction and 40 cm in vertical direction from the dripper during the reclamation process, were investigated. Changes and distribution of soil enzyme activities, also their relationships with principal soil environment factors were studied.

为了解地下水浅埋区重度盐碱地覆膜滴灌对土壤酶活性的影响，测定了重度盐碱地在滴灌利用过程中距离滴头不同水平位置（0～30 cm）和垂直深度（0～40 cm）的土壤碱性磷酸酶、脲酶和蔗糖酶活性，并对土壤酶活性的变化、空间分布特征及与土壤环境因子之间的相关关系进行分析。

Microphylla plantations are obvious, such effects and the contents of soil organic matter, N, P and K are gradually increased with the growth of C. microphylla plantations. Moreover, the activities of phosphomonoesterase, saccharase, protease, urease, dehydrogenase, polyphenol oxidase and nitrate reductase and the contents of edaphon C, N and P are significantly increased at soil depth of 0~30 cm, especially at soil depth of 0~10 cm.

随着小叶锦鸡儿固沙群落发育时间的增长，其综合防风性能提高，土壤有机质、N，P，K含量和生物活性逐渐得到改善。0~30 cm土层中磷酸单酯酶、蛋白酶、脲酶、蔗糖酶、硝酸还原酶、多酚氧化酶、脱氢酶的活性和土壤微生物生物量C，N和P含量均大幅度提高，其中0~10 cm土层增幅最大。

Morphological and physiological identification showed that NTG-01 was Gram-negative, short rod shape, with flagella around the bacteria, V-P test positive and Methyl red test negative. The bacterium can use glucose as carbon suource and produce gases. No H2S produced on TTS medium. Lysine decarboxylase and orthinine decarboxylase, catalase and gelatine test are positive. Arginine decarboxylase, dehydrolase, oxidase and urase test are negative. Sequencing and alignment of 16S rDNA showed that it shared a 99% homologous sequence with Enterobacter aerogenes.

结果显示菌株NTG-01为细菌，革兰氏染色阴性，短杆状，鞭毛周生，V-P试验阳性，甲基红实验阴性，利用葡萄糖产酸产气，不能在TIS培养基上产生H_2S，赖氨酸和鸟氨酸脱羧酶试验呈阳性，精氨酸脱羧酶和双水解酶试验呈阴性，氧化酶和脲酶试验阴性，接触酶和明胶液化试验阳性，但后者反应迟缓，一周后才观测到结果。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

The total number of colony forming units of culturable bacteria and fungi were increased significantly(p.01 in transgenic sugarcane rhizosphere soil, but The total number of CFUs of culturable was not effected. No significantly higher CFUs of bacteria, fungi and actinomycetes resistant to kanamycinwere obtained in transgenic sugarcane soil.

结果表明：转基因甘蔗显著(p.05)降低了土壤脱氢酶的活性，提高了土壤的纤维素酶、蛋白酶和脲酶活性(p.01)，而对蔗糖酶和磷酸单脂酶没有影响；转基因甘蔗显著增加了根际土壤可培养的细菌和真菌数量，但对放线菌影响很小。

The mount of germ and fungus quantity in soil are remarkably increased for the continuous cropping about 5 years, puts the trichobacteria quantity always change tendency to reduce;The edaphon total quantity, the bacterium quantity change reduces generally above the continuous cropping 5 years and below 5 years (including 5 years) the change are not obvious;In the nitrogen physiology group by ammoniates the bacterium primarily and the quantity changes are not very big;The nitrifying bacteria quantity is lower than the comparison (non- continuous cropping), the quantity of denitrifying bacteria is opposite;The alkalinity phosphatase, the urease, invertin activeness have a closed relations with the continuous cropping when it will increase less than 5 years or decrease in more than 5 years. The relation between catalase activeness and the continuous cropping is not very big. The reduction of the invertin activeness, the alkalinity phosphatase, urease activeness have slightly elevates, catalase activeness have no the regular changes.

表现为连作条件下土壤真菌数量显著增大，放线菌数量总的变化趋势降低；土壤微生物总量、细菌数量变化在连作5年以上普遍降低，5年以下(包括5年)则变化差异不明显；氮素生理群中以氨化细菌为主，但数量变化不大；硝化细菌数量低于对照组，反硝化细菌数量则相反：多酚氧化酶、转化酶活性在5年之前逐渐上升，5年之后逐渐下降，，脲酶活性随着连作年限增加持续上升，过氧化氢酶活性、碱性磷酸酶活性变化无规律。

Therewas a significant characteristic of the vertical distribution of soil enzyme activity,the relationship analysis indicated that there was a significant positive relationshipbetween the catalase activity and OM,and total N,and effective P、K,and ex-changible Mg,there was a negative relationship between the catalase activity andBase Situatiou percentage,and Total Contents of Base;the activity of the polyphe-nol oxidase was positively related with OM,and negatively related with total N andexchangible Ca clearly;The urease activity decreased with CPR increasing,and thechange of the former two kinds of soil enzyme actirity was not significantly withCPR increasing,It is possible to be related with maned disturbance frequently.

随着代次增加，有机质和交换性Ca下降明显；土壤的CEC和交换性酸分别下降了8.8—16.1%和6.8—18.5%，因此，其养分总量亦逐代降低，其中有机质下降幅度最大；土壤酶的活性具有明显的垂直分布特征，相关分析表明，过氧化氢酶活性与土壤OM、全N、有效P、K和交换性Mg有极显著的正相关，而与盐基总量、盐基饱和度呈明显的负相关。多酚氧化酶活性与OM呈显著负相关，与全N、交换性Ca有显著相关。脲酶活性则有随连栽代次增加而有下降趋势，前两种酶在代次之间变化不明显，可能与频繁的土壤干扰有关。

The soil microorganisms quantities, microbial diversity index, microbial biomass carbon and the cellulose decomposition strength significantly reduced with increasing of heavy metal concentration, but the soil basal respiration rate were positively enhanced. The soil urease, alkaline phosphatase and polyphenol oxidase in the lead zinc mining area were sensitive. The invertase and catalase were not obvious inhibited to the heavy metals.

该矿区土壤微生物区系组成和微生物活性显著不同于临近非矿区土壤，随着重金属含量的增加，土壤微生物数量、微生物多样性指数、微生物生物量碳以及纤维素分解强度均显著降低，但土壤基础呼吸却明显升高；土壤脲酶、碱性磷酸酶、多酚氧化酶对铅锌矿较为敏感，而蔗糖酶和过氧化氢酶受到的抑制作用不明显。

In order to investigate effects of bound residues of metsulfuron-methyl on the activities of soil enzymes, the activities of urease, acid phosphatase, catalase, and sucrase were determined in Huangfentuspiked with bound residues of metsulfuron-methyl for 98 days.

为了研究甲磺隆结合残留对土壤酶活性的影响，将黄粉土经甲磺隆结合残留处理98天，检测土壤脲酶、酸性磷酸酶、过氧化氢酶和蔗糖酶活性。

Results showed that soil catalase activity was stimulated by the copper products and the inhibition of urease and sucrase activities by copper products were also observed,especially declined significantly in the treatment of BDM,the two enzymes activities in high ...

研究表明：铜制剂对土壤过氧化氢酶活性有刺激作用，而对脲酶、蔗糖酶活性均有显著抑制作用，尤以BDM对土壤酶活性的抑制率最强，高浓度处理下则未能检测出2种酶活性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。