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The cell growth and the releasing rate of urease from bacteria and actinomycete in the presence of different nBPT concentrations were examined.

研究了脲酶抑制剂nBPT对土壤中脲酶活性和细菌、放线菌生长及发酵产酶的影响。

Concanavalin A is immobilized on a pre-activated chitosan microspheres, and then oriented immobilization of urease is carried out based on the strong interaction between ConA and glycoprotein.

将戊二醛将伴刀豆球蛋白和壳聚糖载体交联,然后利用ConA与脲酶糖链的特异性结合作用,实现脲酶的定向固定化。

These two isolates presented typical characteristics of Streptococcus iniae with gram-positive cocci in chains, catalase negative and beta-hemolytic, growth under 10℃ and non-growth up 45 ℃, hydrolyzation of esculin and arginine, Voges-Proskauer, urease, and hippurate tests negative, fermentation of glucose, salicin, sucrose and starch, non-fermentation of arabinose, inulin, lactose, melibiose, raffinose and sorbitol.

实验结果表明,两菌株具有典型的海豚链球菌特性:在显微镜下为球形细胞,呈长短不一的链状排列,革兰氏染色阳性:在血琼脂平板上显示β溶血;1O℃生长,45℃不生长;接触酶阴性;水解七叶苷、精氨酸,VP试验、脲酶和马脲酸试验阴性;发酵葡萄糖、水杨苷、蔗糖和淀粉,不发酵阿拉伯糖、菊糖、乳糖、蜜二糖、棉子糖和山梨醇等。

Immobilization of urease can solve the problem, it have a good stability, and furthermore it can use repeatedly.

固定化可以提高脲酶的稳定性,并可以重复使用,可以很好的克服游离脲酶的缺点。

The results showed that there was a distinctly different effect on urease activity by the three different herbicides.

脲酶在尿素转化过程中起关键作用,当尿素施人土壤后,在脲酶的催化作用下,迅速分解成COz和NH。。

The result indicated the soil catalase and urease activities and the soil lead concentration is extremely remarkable is connected, may express the lead pollution degree; The lead pollution soil after the Digitaria sanguinalis repair, the catalase and urease activities obtains the restoration, is good according to theirs revivification estimate of the situation Digitaria sanguinalis repair effect.

结果表明土壤过氧化氢酶和脲酶活性与土壤铅浓度极显著相关,可表示出土壤受铅污染程度;铅污染土壤经过马唐修复后,过氧化氢酶和脲酶活性均得到恢复,根据它们的活性恢复状况判断出马唐修复效果较好。

Along with soil ecology recovery of tailing mine dam, the urease activity is higher of rhizosphere soil of Miscanthus floridulus Warb,SP2and Eleocharis tuberosa Roemet Schult, while it is low of rhizosphere soil of SP1and Neyraudia reynaudiana.

在尾矿坝土壤生态恢复过程中,五节芒、SP2、莎草科植物的根际土脲酶活性较高,而植物SP1、类芦根际土的脲酶活性较低。

The results showed that granular urea amended with dicyandiamide and N- thiophosphoric triamide induced the highest soil nitrate-reductase activity, granular urea brought about the highest soil urease activity and microbial biomass C a...

结果表明,施用硝化抑制剂和脲酶抑制剂涂层大颗粒尿素肥料的土壤硝酸还原酶活性最高;施用大颗粒尿素,脲酶活性最强,微生物量碳、氮最高。

Results showed that the activity of soil enzymes was obviously different.Among the different vegetation types,the activity of soil enzymes under Tilia mandshurica forests was the highest except peroxidase,whereas that under shrub-grass was the lowest.The activity of soil urease and alkaline phosphate under shrub was higher than that under the mixed forests.Activity of soil catalase and phosphate under Larix principis-rupprechtii forests was higher than that under Pinus tabulaeformis.

结果表明:不同植被类型的土壤酶活性有显著的区别,其中除过氧化物酶外,糠椴林的土壤酶活性均属最高水平,而灌草丛所有酶活性都属最低水平;灌木丛中脲酶和碱性磷酸酶活性显著高于杂木林;华北落叶松林的磷酸酶和过氧化氢酶活性均高于油松林。

The kinetics of soil urease differed with soil types. The urease in black soil and albic soil had larger values of Km and V than those in brown soil and cinnamon soil, but V/ Km had no significant differences, indicating that the kinetic parameters of soil urease were significantly influenced by soil type.

黑土和白浆土脲酶Km和V值比棕壤、褐土大,而V/Km的变化不明显,说明土壤类型对脲酶动力学参数有显著影响。

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