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The role of arginine to assist protein refolding was similar to that of acetone, both of them were able to combine with the denature lysozyme and improve the refolding yield.

结果表明,与自发复性收率(40%)相比,复性液中添加精氨酸、甘油、乙酰胺、丙酮、硫脲、海藻糖和甜菜碱均能有效提高变性溶菌酶的复性收率。

Abstract]Objective: To explore whether chronic administration of methylamine of mini_dose in Wistar rats might induce the elevation of semicarbazide_sensitive amine oxidaseactivity and initiate endothelial injury.

目的:探讨Wistar大鼠体内低剂量甲胺的慢性分布是否能导致氨基脲敏感型胺氧化酶活性上调而诱导内皮的损伤。

SD rats were peritoneally injected with streptozotocin to establish diabetic animal model.The changes of nonenzymatic glycation and peroxidation in rats supplemented with vitamin C and/or vitamin E during five weeks were investigated.

以链脲佐菌素腹腔注射SD大鼠建立糖尿病动物模型,在纯营养素饲料配方中补充维生素C和/或维生素E,喂饲5周,观察单独或联合补充维生素C、维生素E对糖尿病非酶糖化及过氧化的干预作用。

Methods : The levels of fasting blood glucose and fast plasma insulin, the activities of superoxide dismutase and glutathione peroxidase and level of lipid peroxidate were measured in the pancreas, heart and liver of normal and streptozotocin-induced diabetes-prone mice exposed to multiple stressors for 6 weeks. Results : The FBG level in STZ mice was elevated by stress, while the FINS level was not increased.

用链脲佐菌素复制糖尿病倾向小鼠模型,予以6周慢性多相性应激,检测其空腹血糖、空腹胰岛素水平和胰、心、肝组织超氧化物歧化酶、谷肽甘肽过氧化物酶活性及脂质过氧化物含量。

AIM: To assess the effect of semicarbazide hydrochloride, the blocker of synthetic enzyme for GABA, on the hypnotic activity of melatonin.

目的:观察GABA合成酶抑制剂盐酸氨基脲对褪黑激素催眠作用的影响。

Objective Through observing the ei'fect of the TCM decoction TangShenHuaYuJian on the kidney weight, index of kidney hypertrophy(kidney weight/body weight), blood glucose , blood urea nitrogen, creatinine, blood advanced glycation end-products, advanced glycation end-products in kidney tissue in the earlier stage of diabetic nephropalhy rats induced by injecting 3 -cytotoxins into abdomen, to explore the relationship between nonenzymatic glycosylation and diabetic nephropathy, and provide an experimental basis for clinical application and for further studying TSHYJ.

目的 通过观察糖肾化瘀煎对长期高血糖所致的早期粮尿病肾病大鼠(腹腔注射链脲佐菌素后诱导所形成)肾重、肾重指数、血糖、血尿素氮、肌酐、血糖基化终末产物AGEs、肾组织糖基化终末产物AGEs等指标的影响,从非酶糖基化角度探讨此制剂对糖尿病肾病早期的作用机理,并为糖肾化瘀煎的临床运用和进一步开发提供实验依据。

And finally, a suggested refolding mechanism of reduced-denatured egg white lysozymes in urea solution was presented.

最后,给出了一个建议的还原变性蛋白溶菌酶分子在脲溶液中的折叠机理。

Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.

最后,给出了一个建议的还原和非还原蛋白溶菌酶在脲溶液中的重折叠过程。

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses.

通过还原和非还原蛋白电泳,研究了还原变性蛋白溶菌酶在脲溶液中重折叠过程的集聚相互作用。

The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a "phase diagram" method of fluorescence.

用荧光&相图&法研究了还原和非还原脲变性蛋白溶菌酶的重折叠。

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