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With the sample dissolved in 0.1NNaOH solution and bovine serum albumin used as the standard protein, the Percentage of proteins in the sample is given by spectrophotometric method with biuret reagent.

该法使用0.1NNaOH溶液溶解样品,以大牛血清白蛋白作为标准蛋白质,利用缩二试剂分光光度测定样品中的蛋白质含量。

On the basis of Kjeldahl, Coomassie Brilliant Blue ,Folin-hydroxybenzene,Biuret was used to determine the human-like collagen produced from the recombined E.

以凯氏定氮法为基准方法,采用考马斯亮蓝法、Folin-酚法、双缩法对从重组大肠杆菌发酵产物中提取的类人胶原蛋白含量进行了测定。

Through analysis on the technology characteristic and the main operating point in the different parts of process, such as granulation system temperature control, biuret concentration control, aminated triple superphosphate, dedusting and washing system, raw material distribution system and so on, the advantage of high-nitrogen compound fertilizer produced by K-T process is remarkable.

介绍尿基复合肥K-T技术工艺流程;通过造粒,系统温度控制,缩二含量控制,重钙的氨化,除尘洗涤系统,配料系统等方面的技术特点,操作控制要点的分析,指出K-T工艺生产高氮复合肥的突出优势。

Key words: compound fertilizer; determination; biuret; spectrophotometry; interference matter

濮阳市氯碱厂,河南濮阳 457000 复合肥;测定;缩二;分光光度法;干扰物质

The characters and applications of vacuum film evaporation and molecular evaporation are outlined, and an XZN inner-cooling vacuum agitated film evaporator was used to reduce free monomer from HDI biuret , which is for improving the quality of polyurethane paints and coatings, decreasing pollution and danger to worker.

本文介绍了真空薄膜蒸发和分子蒸发的原理、特点和应用,并介绍了真空薄膜蒸发-冷凝工艺在分离HDI 缩二中游离单体中的应用。

Methods Determine the marker of hepatitis B by ELISA, total protein by biuret method, albumin by bromocresol green method, and the component of serum protein by agarose gel electrophoresis.

采用酶联免疫吸附实验检测乙肝标志物,双缩法检测总蛋白,溴甲酚绿法检测白蛋白,琼脂糖凝胶电泳法分析血清蛋白组分。

Gelatin-microsphere was prepared by emulsion chemical-crossline technique. With the hydrolytic agent of citric acid, the effect of different hydrolyzed time, temperature and pH on the gelatin hydrolyzing degree and GMS was studied by the method of biuret.

采用乳化交联法制备明胶微球,以柠檬酸为水解促进剂,利用双缩法研究了反应时间、温度及pH值对GMS及明胶水解程度的影响。

We present a simple approach to determine the content of casein in crosslinked polyacylamide, i. e., protein molecules are complexed with copper ions by biuret reaction. The polymer containing proteins is cinerated at high temperature and then the residue is dissolved into nitric acid. Protein content is measured by determining concentration of copper ion in nitric acid solution using atomic absorption spectrum.

本文提出了一种在交联聚丙烯酰胺中测定酪蛋白的简便方法,即:利用双缩反应使蛋白质与铜离子络合,含蛋白质的聚合物在高温下灰化,残留物溶于硝酸,通过用原子吸收光谱测定硝酸溶液中的铜离子浓度来测量蛋白质的含量。

The equation of rapid determination sesame cake protein by improving biuret method is as follows: Y=0.1559X+0.0012, the protein concentration range from 0.75 mg/mL to 3.78 mg/mL displayed better linear with absorption value.

改良双缩法快速测定饼粕蛋白提取率的方程为:Y=0.1559X+0.0012,蛋白浓度在0.75~3.78mg/mL之间与吸光度值成良好线性。

The absorbance reproducibility of the samples was good, RSD=0.99%(n=6),the average recovery was 96.67%,RSD=0.45%(n=6). There had a difference between biuret menthod and Folinlowry.

结果 双缩法快速、简便,得到样品的含量为47.21%,精密度实验RSD=0.20%(n=6),样品重现性好,RSD=0.99%(n=6),平均回收率为96.67%,RSD=0.45%(n=6);两种方法测定之间有差异。

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