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脱镁叶绿素

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Nine kinds of pigments were identified in Ulothrix sp.. They are carotinoid, pheophytin, chlorophyll a, b, chlorophyllin chlorophyllide a,neoxanthin, chlorophyll c and pheophytin c.

丝藻的色素种类有 9种,分别为胡萝卜素、脱镁叶绿素 a、叶绿素 a、叶绿素 b、叶绿酸、脱酯基叶绿酸、新黄素、叶绿素 c和脱镁叶绿素 c。

The results showed that the contents of chlorophyll a and chlorophyll b decreased,and contents of pheophytin a and pheophytin b increased, but chlorophyllide a and chlorophyllide b changed slightly.

结果表明,随着加工过程的推进,叶绿素a、叶绿素b呈下降趋势,脱镁叶绿素a、脱镁叶绿素b呈上升趋势,叶绿素酸酯a、叶绿素酸酯b变化较小。

This and previous studies have shown that pheophytin absorption peaks appears at 412 nm in the violet region of spectrum, and 670 nm in the red.

由实测数据及相关文献推断出脱镁叶绿素蓝光和红光吸收峰分别位于412和670nm波段,并采用多元线性回归对脱镁叶绿素和叶绿素a的比吸收系数进行研究。

Additionally, Pb-pheophytin was non-fluorescent and could result in the fluorescence intensity quenching of pheophytin.

通过光度动力学测定,分别求得铅与脱镁叶绿素a、脱镁叶绿素b和13〓-羟基脱镁叶绿素a的反应速率常数为2.0、0.78和1.86mol〓 ls〓。

The multivariate linear regression analysis on specific absorption coefficients for pheophytin and chlorophyll a indicates that the specific absorption coefficients for pheophytin was much larger than chlorophyll a at 412 nm; slightly larger than pheophytin at 440nm; and about 3 times larger than pheophytin at 670 nm and 675 nm.

结果表明,412nm波段脱镁叶绿素的比吸收系数远大于叶绿素a;440nm波段,叶绿素a的比吸收系数略大于脱镁叶绿素;670、675nm波段,叶绿素a的比吸收系数约为脱镁叶绿素的3倍。

Accordingly, the Q bands of them blue shifted by 6mn and 13nm, respectively. The fluorescence emission wavelengths of Cd-pheophytin a and Cd-pheophytin b observed at 660nm and 649nm, respectively, which were shorter than those of corresponding pheophytin a and pheophytin b by 10nm and 9nm, respectively.

研究发现:在光吸收性质上,与脱镁叶绿素a和b相比,镉叶绿素a和b在Soret带的吸收峰位置分别红移了19nm和11nm,而在Q带则分别蓝移了6nm和13nm;在荧光性质上,镉叶绿素a和b的荧光发射波长比脱镁叶绿素a和b分别蓝移了10nm和9nm。

While the proportion of pheophytin in total pigment concentration increased, the absorption peak in the blue region of spectrum deviated from 440 nm to near 412 nm. The absorption peak height in the blue region was mainly influenced by pheophytin concentration, whereas the height in the red was decided more by chlorophyll a concentration.

脱镁叶绿素占色素总浓度比例的增大,浮游植物吸收曲线上蓝光吸收峰偏离440nm波段,逐渐向412nm波段靠近,并得到蓝光波段吸收峰的高度由脱镁叶绿素浓度决定,而红光波段吸收峰的高度由叶绿素a浓度决定。

Successful separation of Cd-pheophytin a, Cd-pheophytin b, chlorophyll a, chlorophyll b, pheophytin a and pheophytin b in 14min was firstly accomplished by reversed-phase HPLC at a flow rate of 0.8ml/min, with the mobile phase of methanol: acetone (80: 20, v/v).

首次以甲醇:丙酮=80:20为流动相,流速为0.8ml/min,在C〓柱(5μm,4.0×250mm)上,14min内成功实现了镉叶绿素a和镉叶绿素b配合物与叶绿素a,b和脱镁叶绿素a,b的高效液相色谱分离。

The excitation and fluorescence spectra were taken with leaves and the relevant molecule samples prepared in thelaboratory,including Chla,pheophytin Aand pheophorbide A.

为此我们从海带里提取Chla,然后采用分子修饰的方法对Chla分子进行一步步修饰分别生成脱镁叶绿素A和脱镁叶绿素甲酯一酸A。

The absorption bands observed for Pb-pheophytin a at 462nm and 673nm , while for Pb-pheophytin b at 480nm and 662nm . Compared with pheophytin a and pheophytin b, all these bands red shifted more or less. Among the reported metallochlorophylls, the degree of red shift of Pb-pheophytin in Soret band was the largest by 54nm and 44nm for Pb-pheophytin a and Pb-pheophytin b, respectively.

实验表明:对铅叶绿素a和b系列来说,它们在Soret带、Q带的特征吸收峰分别在462nm,673nm和480nm,662nm;与脱镁叶绿素a,b相比,铅叶绿素a,b在Soret带和Q带吸收峰均有不同程度的红移;与现已报道的金属叶绿素相比,铅叶绿素a,b在Soret带的红移程度最大,分别为54和44nm;铅叶绿素不具有荧光活性,并能猝灭脱镁叶绿素的荧光。

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