脱色的

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product.

具体为：首先将交联剂、功能单体、模板分子和引发剂溶解在致孔剂中，得到混合溶剂；将混合溶剂超声脱气；混合均匀后，通入氮气，氮气状态或抽真空状态下密封；进行聚合反应，分子印迹聚合物采用热引发；聚合反应结束后，将合成的分子印迹聚合物取出研磨、过筛后用有机溶剂除去模板分子，通过高效液相色谱-紫外检测，直到检测不到模板分子；将除去模板分子的聚合物，真空干燥，得到利血平特异的分子印迹聚合物。

Different concentrations of glucose (0.10%, 1.50%, 2.50%, and 4.25%) and LPS (0, 0.1, 1.0, 10.0, 50.0, and 100.0 mg/L) were added into culture media. Then PMCs were incubated in culture medium containing 10.0 mg/L of LPS and 4.25% of glucose for 0, 1, 3, 6, 12, and 24 hours. The hexokinase activity of PMCs was measured with standard glucose-6-phosphate dehydrogenase (G6PDH)-coupled enzymatic assay.

首先将含不同浓度的葡萄糖（0.10%、1.50%、2.50%、4.25%）分别和不同剂量的LPS（0、0.1、1.0、10.0、50.0、100.0mg/L）加入培养基；其次用含4.25%葡萄糖和10.0mg/L LPS的培养基孵育细胞不同时间（0、1、3、6、12、24h）；6-磷酸葡萄糖脱氢酶耦联比色法测定HK活性。

The alkali dosage depends mainly on the constituents and property of material and the quality requirements for pulp: more alkali dosage will speed up cooking process, reduce the hardness of pulp and improve the bleachability of pulp; but excessive alkali dosage will lower the pulp yield and physical intensity, while too less alkali dosage will result in harder and darker pulp, uneasy to bleach, too much screenings, and even extending cooking time cannot guarantee the reaction of delignification.

用碱量主要取决于原料的组成和性质及对纸浆的质量要求：加用碱量有利于加快蒸煮速度降低纸浆硬度和提高纸浆的可漂性：但过高却会降低纸浆得率和物理强度，如过低时，成浆较硬而色暗，不易漂白而且筛渣多即使延长蒸煮时间也难以保证脱木素反应进行。

The lumped kinetic model of monoterpene, abietic type resin acid, pimaric acid type resin acid, p-cymene, hydro-monoterpene, dehydro-abietic acid, hydro-abietic type resin acid, and hydro-pimaric acid type resin acid was established based on the structural groups and approximate reaction kinetic theory according to the fundamental principles of the lumped method.

在消除内、外扩散影响的条件下，在线跟踪反应产物并用气相色谱法测定反应体系组成随时间的变化，构造了Pd／C催化剂上松脂分子间氢转移集总反应网络，建立了包含单萜烯、枞酸型树脂酸、海松酸型树脂酸、对伞花烃、氢化单萜烯、脱氢枞酸、氢化枞酸型树脂酸和氢化海松酸型树脂酸等复杂反应体系的集总动力学模型，并估算了模型参数。

For this, I pretended to whispered unintendedly, I got depressed from playing cards with the boring guys today where nearly all my clothes were taken off in losing.

我于是联想到很多小说中的情节——一个女人被色狼们在牌桌上赢到衣服被一件一件脱去，变态的人也许比较喜欢猎物被征服后自己无奈地就范吧。

Heidelberg will display its Anicolor technology (the zoneless short inking unit with dampening system) on a four-colour Speedmaster SM 52 with an inline coating unit.

海德堡将展出的Anicolor技术（ zoneless短期脱墨单位与抑制系统）的四色速霸52钐与内嵌涂层单位。

The effects of kelp extracts,β-cyc1odextrin and tea polyphenol in deodorizing were discussed.

建立了高效液相色谱法测定大蒜臭味物质含量的方法，并研究了海带提取物、茶多酚、β-环糊精对大蒜臭味的脱除效果。

This design main realization escapes the fabric cotton seed color to choose the system on position machine and the lower position machine communication.

本设计主要实现脱绒棉种色选系统的上位机与下位机的通讯。

The neurons were continuously cultured in the culture medium with radix astragali (100 mg/L) or without it. The cellular morphology, A value and effluxes of LDH and K+ from cells were measured in the radix astragali group and the control group respectively.

用氰化钠造成体外培养新生大鼠大脑皮层神经细胞缺氧模型，比较黄芪组和对照组的细胞形态、存活细胞数(四唑盐微量自动比色检测A值)、乳酸脱氢酶值和钾离子流出的变化。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。