脱细胞的

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

Our result showed the expressed HμOR desensitization could be induced by acute stimulation of μ-selective agonists DAGO and Ohm in a dose-dependent manner in G protein level.

在昆虫细胞表达的HμOR，用μ选择性激动剂DAGO和Ohm急性预处理，也同样导致HμOR的脱敏，并且随着预处理激动剂浓度增加，脱敏现象愈明显。

This paper was also studied primarily on the decolor of PHA. Physical and chemical methods were all used to decolor the organic phase after the recovery by CHC13 from the cells.

另外，本论文对PHA的脱色作了初步探索，实验就物理方法和化学方法针对氯仿提取细胞后得到的PHA有机相进行了脱色处理。

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明：纤毛虫形成包囊过程中，皮层细胞质中产生具有自噬泡消化特征的细胞器，将部分纤毛结构等逐步消化；休眠包囊中，表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体，大核染色质和核仁颗粒变小，核孔增大，并发生染色质附着于核孔内膜的现象；细胞脱包囊期间，皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化。

Methods: Cell proliferation was measured by crystal voilet staining assay. The activity of lactic acid dehydrogenase in the upper layer of culture solution was tested to examine the damage to the cell membrane.3H-TdR was used to study the effect of porphyrin nitrogen mustard on the synthesis of DNA.

用结晶紫染色法测定卟啉氮芥对人肝癌细胞SMMC7721的光动力抑制效应，用测定培养上清乳酸脱氢酶活性方法研究光动力作用对人肝癌细胞SMMC7721细胞膜的作用，用3H-TdR掺入法研究光动力作用对人肝癌细胞SMMC7721DNA合成的影响。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外，为了证实其脂肪代谢产物胆酸对细胞癌化的影响，亦使用SW480和HCT116细胞株，经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后，其细胞增生比率分别有12.35%，20.08%，29.69%，33.56%，45.85%和54.32%(p.05)以及3.69%，5.59%，8.52%，12.26%，16.52%和19.71%(p.05)上升情形，并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A：氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

OBJECTIVE: To explore the feasibility of adipose-derived stromal cells in repairing loaded bone defects through observing the repair of rabbit tibial bone defects with adipose-derived stromal cells that was cultured to osteoplast and bovine decalcified bone composite.

目的：通过兔脂肪基质细胞诱导培养为成骨细胞，并与牛脱钙骨复合培养，对兔胫骨骨缺损修复情况的观察，探讨脂肪基质细胞修复骨缺损的可行性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。