脱细胞的

About 4 days post amputation, cells in some areas in the out epidermis and inner parietal peritoneum near the end of the stump are induced by injury to dedifferentiate into stem cells and then proliferate forming stem-cell masses, which then invaginate into the interior dermis. The dermal cells are then induced by those invaginating stem-cell masses to dedifferentiate.

从第4 d起，近创伤处的表皮层和体腔上皮中部分细胞发生了脱分化形成了干细胞，并增殖形成了干细胞团，然后向真皮层内陷，并诱导真皮层组织发生脱分化，最终在残腕体壁的不同部位形成了众多干细胞原基，是进一步再生所需的细胞库。

The activity of superoxide dismutase in the myocardial cells was determined by the diphenol method, plasma malondialdehyde content was measured by TBA assay, the culture medium LDH content was measured by the acetonic acid method, the intracellular calcium concentration was measured by fluorescence spectropho- tometer- The results was shown that aspartate-K.

结果显示，门冬氨酸钾镁能减少阿霉素中毒心肌细胞乳酸脱氢酶的释放量，降低细胞内丙二醛含量及游离钙浓度，同时可升高细胞内超氧化物歧化酶活力；结果表明，门冬氨酸钾镁可能通过抗脂质过氧化及逆转细胞内钙离子过负荷作用进而保护中毒心肌细胞。

MATERIALS: The demineralized bone was prepared with cancellated bone of metaphysis from newborn calf via treatment of decalcification, defatting, acellular, remove antigen, and deproteinization.

材料：取新生小牛四肢长骨干干骺端的松质骨置于脱钙液中脱钙、脱脂、去细胞、去抗原和去蛋白等程序处理制备成脱钙骨支架。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or P .01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or P .01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05， P 。01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05， P 。01）；4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or 0.01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or 0.01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05或0.01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05或0.01）；4种单体对过氧化氢损伤无显著的作用。

The fluorescence silgnal on Thr11-phorphoslated histone H3 reached the strongest at early metaphase at the centromere punctated in the central of mitotic cell.

结果显示：在MCF-7细胞中，组蛋白H3 Thr11的磷酸化发生在早前期细胞染色体的着丝粒处，成点状分布，继而在早中期达到最高水平，并眨羔状集中在赤道板上，在有丝分裂后期开始脱磷酸化，并于末期完成脱磷酸化。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增，用脂质体介导的方法转染人骨发生蛋白2基因，将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料，连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体，放射线和病理观察。

Five hours after the noise exposure, the cochleae were harvested. mitochondria energetic function, succinate dehydrogenase activity, in the hair cells of cochleae was evaluated with a colorimetric assay using blue tetrazolium monosodium salt. the sdh-labeled organs of corti were double stained with propidium iodide, a dna intercalating fluorescent probe used to visualize the morphologic viability of hair cell nuclei.

采用琥珀酸脱氢酶（succinate dehydrogenase，sdh）染色法进行耳蜗基底膜细胞线粒体染色，细胞核dna荧光染料碘化丙啶（propidium iodide，pi）双重染色耳蜗基底膜，以未受噪声暴露动物为对照，显微镜下观察噪声暴露后耳蜗基底膜核固缩和核肿胀毛细胞琥珀酸脱氢酶染色的变化。

The residual calcium and phosphorus contents of bone particles were measured after degreased and demineral ized.

取猪四肢长管状骨皮质骨经理化处理后，制备脱脂脱钙和脱脂脱钙去细胞两种粒径为250 ～ 810 μm 的DBM 颗粒和浸提液，骨颗粒脱脂脱钙后测钙、磷含量。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。