脱氧核糖核酸酶

Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.

利用随机引物逆转录合成互补脱氧核糖核酸，根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。

Methods The model of subacutely aging rats were made by injecting Dgal into the rats, abdominal cavity continually. After lavaging HSWY, the apoptosis rate and the levels of Fas and FasL mRNA were measured by TUNEL and RTPCR; the expressions of Fas/Fasl protein were detected by SP immunocytochemistry.

采用D半乳糖连续腹腔注射致亚急性衰老大鼠模型，同时以何首乌饮灌胃作为延缓衰老组；造模后应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法检测各组大鼠卵巢细胞凋亡情况；测定各组Fas和FasL的信使核糖核酸水平；SP免疫组化法检测卵巢细胞Fas和FasL蛋白的表达情况。

What is the function of ligase in DNA replication?

什麽是连接酶的作用在脱氧核糖核酸复制的？

The number of 5-Br deoxy uridine monophosphate and nestin in subventricle zone and dentate gyrus of rats at 3, 5, 7, 14, 21 and 30 days after MCAO were detected by immunohistochemistry and the bFGF mRNA were detected by RT-PCR at the same time.

免疫荧光法检测各组大鼠缺血再灌注损伤后第3、5、7、14、21、30天病灶侧侧脑室下区、海马齿状回区5-溴脱氧尿嘧啶核苷和Nestin的变化，逆转录聚合酶链式扩增方法检测相应时间点病灶侧碱性成纤维生长因子信使核糖核酸的表达。

What is the function of DNA polymerase in DNA replication?

什麽是脱氧核糖核酸聚合酶的作用在脱氧核糖核酸复制的？

Hepatitis B virus ; Breast-feeding ; Polymerase chain reaction; Deoxyribonucleic acid

乙型肝炎病毒；母乳喂养；聚合酶链反应脱氧核糖核酸

Key words］ Digestive system neoplasms;Deoxyribonuclease;carcinoembryonic antigen;Diagnostic techniques,digestive system

关键词］消化系统肿瘤；脱氧核糖核酸酶；癌胚抗原；诊断技术，消化系统

Deoxyribonuclease of Ganoderma lucidum is characterized in that the molecular weight is 13,807Da, N-terminal amino acid sequence is PLDTGRYHIY, and the deoxyribonuclease has two peptide fragments of QVETVVDRLDELPIR and ESPGLQGVSSVPLR.

灵芝脱氧核糖核酸酶的分子量为13807Da、N-端氨基酸序列为PLDTGRYHIY、具有QVETVVDRLDELPIR和ESPGLQGVSSVPLR两个肽段。

Deoxyribonuclease for cystic fibrosis.

脱氧核糖核酸酶为囊性纤维化。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。