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Bcl-2 mRNA was detected by RT-PCR in all cells;(2)After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:(1)Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.

分离对照组及实验组14 d组大鼠的肝细胞行原代培养后,使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后,用FCM法测定肝细胞的凋亡比率,用TUNEL技术进行肝细胞凋亡的原位检测。

During years of growing activity, Varese plant has pursued

瓦雷泽工厂几年来一直从事核糖核酸和脱氧核糖核酸衍生物的

Objective: The aim of this study was to investigate the effects of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinon the growth of nude mice cervical cancer, and to explore the possible mechanism of ginsenoside Rg3 and ginsenoside Rg3 combined with cisplatinin cells and molecules levels. The growth of human cervical cancer Hela cell, apoptosis of tumor cells , expression of CD31and PeNt were observed with optic microscope, radio-immunity immunological histo chemistry, terminal deoxynucleotidy transferase-mediated dUTP nick end labeling, and immunohistochemical staining. The purpose of this study is to provide a new way of medication to eure cervical cancer in clinic.

目的:本实验通过建立子宫颈癌裸鼠病理模型后,以此为研究对象,运用光镜、免疫组化及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记技术,观察人参皂甙Rg3[20—Ginsenoside Rg3]和顺铂单独及二者联合应用对人宫颈癌Hela细胞裸鼠移植瘤生长的影响,重点研究肿瘤的生长、肿瘤细胞的凋亡及PCNA、CD31的表达情况,以期从细胞及分子水平揭示人参皂甙Rg3和顺铂单独及二者联合应用对人宫颈癌Hela细胞的作用机制,为临床上药物治疗与预防宫颈癌寻找更加完善的途径。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。