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Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4/brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸,干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4/brn4的表达,免疫印迹检测反义干扰细胞POU3F4/brn4蛋白条带的强弱,RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化,观察POU3F4/brn4对COCH基因的转录调控作用。

The pathogen of gas gangrene mainly exist and live in local place, and seldom enter blood system to cause blood poisoning, but than can form lots of toxins, this toxins can harm blood system, kidney system, and also the tissue can putrescence and the wound will enlarge step by step, all tissue putrescence and toxin can make these bacteria easier to live and reproduce, all these situation if not being control in time will lead a serious situation that multiple organs dysfunction and then died

气性坏疽的病原菌主要在伤口内生长繁殖很少侵入血液循环引起败血症。产气夹膜杆菌产生α毒素、胶原酶透明质酸酶、溶纤维酶和脱氧核糖核酸酶等,红细胞破坏引起溶血血红蛋白尿、尿少、肾组织坏死水肿、液化,肌肉大片坏死使病变迅速扩散、恶化。糖类分解产生大量气体使组织膨胀;蛋白质的分解和明胶的液化,产生硫化氢,使伤口发生恶臭由于局部缺血,血浆渗出,及各种毒素的作用伤口内的组织和肌肉,进一步坏死和腐化,更利于细菌的繁殖使病变更为恶化。

Stereoselective synthesis of 2-deoxy-β-glycosidic linkage has been found to be one of the most challenging tasks in the synthesis of these natural products.

在合成这些天然产物时,立体专一的合成2—脱氧β糖苷是其中的一个难题。

In order to verify the efficiency of DONtest-HPLC in breeding wheat with low DON, and to reveal the variation of DON content in wheat,8 cultivars with different resisance to fusarium head blight were used for analysis when fusarium head blight epidemic severely occurred in the middle and lower stretches of Yangtze River in 2003. The results indicated that DONtest-HPLC was a kind of quick and accurate method in detection of DON content with a recovery rate 78.2%in average.

以2003年长江中下游地区赤霉病大流行后收获的8个不同抗、感赤霉病小麦为材料,探讨DONtest-HPLC在低毒素小麦选育中的利用价值,并揭示不同抗性小麦脱氧雪腐镰刀菌烯醇含量的差异特点。

Head blight not only causes the severe grain yield loss, but also causes the harvested grain contaminated with several mycotoxins, such as deoxynivalenol and zearalenone. The consumption of food product made from grains contaminated with these toxins is a potential problem for human and farm animals, which is known to cause carcinogenic, genotoxic acute or chronic diseases or to directly target the kidney, liver or immune system, and has caused great concern of food safety.

赤霉菌引起小麦的穗腐,不仅使小麦籽粒产量降低、品质变劣,而且病麦粒中存留有病菌产生的真菌毒素,如脱氧雪腐镰刀菌烯醇(Deoxynivalenol,DON)和玉米赤霉烯酮(Zearalenone,ZEN),食用污染毒素的病麦及其制成品后会引起人畜中毒,还有致癌、致畸和诱变的作用。

5In the 5 million years since we hominids separated from apes .

在从类人猿进化成人以来这五百万年的时间里,我们人类的脱氧核糖核酸的进化程度还不足2%。

In the last part in the paper, interrelationship of oxide inclusion and hydrogen in Al melt is examined.

工业上通常使用的普N〓不再需要脱氧和脱水工艺,其应用前景极为乐观。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只,以钥孔虫戚血蓝蛋白(keyhole limpet hemocyanin,KLH)全身免疫后,经耳蜗底回钻孔以相同抗原进行内耳免疫,然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdUrd)后处死动物,取颞骨经组织学处理制作冰冻切片,用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

What is the function of ligase in DNA replication?

什麽是连接酶的作用在脱氧核糖核酸复制的?

U.S. and Japanese scientists medaka fish through the analysis of deoxyribonucleic acid all of the information and found that the structure of DNA nucleosome affect DNA variation, thus affecting the biological evolution.

美日两国科学家通过分析青鳉鱼脱氧核糖核酸的全部信息,发现DNA核小体结构影响着DNA变异,从而影响生物进化。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。