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By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段,用双脱氧链终止银染测序法测定了该片段的全序列,发现它含有一个完整的开放阅读框,编码299个氨基酸残基的多肽,分子量约35KD。

The detection of apoptotic cells and expression of the hyaluronic acid binding CD44-receptor were determined by flow cytometry.

细胞的增殖通过3H-脱氧胸苷酸掺入的方法检测;细胞凋亡及透明质酸结合受体CD44的表达用流式细胞仪检测。

Gated ~(99)Tc~m-hexakis-2-methoxy-isobutyl isonitrile /~(18)F-fluorodeoxyglucose single photon emission tomography with dual isotope simultaneous acquisition can provide the information about left ventricular function, myocardial perfusion and metabolism simultaneously.

~(99)Tc~m-甲氧基异丁基异腈和~(18)F-脱氧葡萄糖双核素同时采集单光子计算机断层显像可以一次显像获得左心室心肌的血流灌注和葡萄糖代谢的情况,如果加上门控采集,可以同时了解左心室功能的信息。

ABSTRACT Gated 〓Tc〓-hexakis-2-methoxy-isobutyl isonitrile /〓F-fluorodeoxyglucose single photon emission tomography with dual isotope simultaneous acquisition can provide the information about left ventricular function, myocardial perfusion and metabolism simultaneously.

Tc〓-甲氧基异丁基异腈和〓F-脱氧葡萄糖双核素同时采集单光子计算机断层显像可以一次显像获得左心室心肌的血流灌注和葡萄糖代谢的情况,如果加上门控采集,可以同时了解左心室功能的信息。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外,为了证实其脂肪代谢产物胆酸对细胞癌化的影响,亦使用SW480和HCT116细胞株,经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后,其细胞增生比率分别有12.35%,20.08%,29.69%,33.56%,45.85%和54.32%(p.05)以及3.69%,5.59%,8.52%,12.26%,16.52%和19.71%(p.05)上升情形,并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A:氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

Objective To study partial biochemical characterization and effect of lumbricus dornases.

:目的研究地龙体内两种新型脱氧核糖核酸酶的部分生化性质,并对其作用进行初步探索。

Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein and neuronspecific enolase of 5bromo2deoxyuridinelabeled MSCs were detected by immunohistochemical double staining.

线栓法建立左侧大鼠MCAO模型,随机分为3组:手术组、MSCs 移植组、生理盐水组。5溴2脱氧尿核苷标记的MSCs移植后,采用改良神经功能损伤评分系统评价大鼠神经功能恢复情况;应用免疫组织化学双染技术检测MSCs的存活、迁移及其巢蛋白、胶质纤维酸性蛋白和神经元特异性烯醇化酶的表达。

Three organic compounds containing nitrogen have been isolated fTom soft coral Sarcophyton Molle Collected from South China Sea. Their structures have been determined by UV, IR, MS, 1H NMR and 13C NMR as sinumeramide Thymine and Thympidine.

从中国南海软珊瑚中分离到三个含氮化合物,其结构经紫外光谱、红外光谱、质谱和核磁共振(1HNMR、13CNMR)分析分别确定为神经酰胺Sinumeramide,胸腺嘧啶和脱氧尿核嘧啶苷。

The DFT (B3LYP/6-31+G**) method was used to study the conformations and isomerization of the isolated and monohydrated 2',3'-dideoxy-2',3'-didehydroguanosine (D4G) molecules in gas phase.

采用密度泛函方法在B3LYP/6-31+G**水平上研究了2',3'-二脱氧-2',3'-二去氢鸟嘌呤核苷分子(D4G)的构象。

The DFT B3LYP/6-31+G(superscript ** method was used to study the conformations and isomerization of the isolated and monohydrated 2', 3'-dideoxy-2', 3'-didehydroguanosine (D4G) molecules in gas phase.

采用密度泛函方法在B3LYP/6-31+G水平上研究了2',3'-二脱氧-2',3'-二去氢鸟嘌呤核苷分子(D4G)的构象。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。