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During encystment, organelles displaying characteristics of autophagic vacuole appear in the cytoplasm of the cortex and part of ciliate structures and other cell components are digested by these organelles.

以营养期纤毛虫、人工诱导形成包囊和脱包囊的纤毛虫以及纤毛虫休眠包囊为材料,研究形成NKR包囊的纤毛虫在不同生理状态下细胞亚显微结构的分化。

A large scope differentiation and a local differentiation occurred during the course of encystment in Tachysoma pellionella and in Euplotes encysticus respectively.

急纤虫形成毛基体吸收型包囊时,细胞结构发生了大范围的脱分化过程。

In resting cysts, the microtubules under the pellicle, cortical ciliatures, conjunctions between cilia basal bodies, supporting structure of basal bodies and other microtubule structures are absorbed in different extent; microtubule-like assemble bodies appear at the center of the remained cilia basal bodies; the granules of chromatin and nucleolus in macronucleus become smaller whereas nucleus pores of the micronucleus become bigger; the inner membrane of some nucleus pores are attached with chromatin.

结果表明:纤毛虫形成包囊过程中,皮层细胞质中产生具有自噬泡消化特征的细胞器,将部分纤毛结构等逐步消化;休眠包囊中,表膜下微管、皮层纤毛器和纤毛基体间连接、基体托架及其他微管结构不同程度地被吸收、在残剩纤毛基体的中央形成微管形结构聚合体,大核染色质和核仁颗粒变小,核孔增大,并发生染色质附着于核孔内膜的现象;细胞脱包囊期间,皮层细胞骨架、线粒体等经历了在残剩结构基础上的再分化。

The former formed a kind of KR cyst, while the later formed a kind of NKR cyst. All the kinetosomes, ciliary shafts and microtubules were resorbed in the former, meanwhile, the cell lost most water and cell volume decreased distinctly. In the later, only part of kinetosomes and part of microtubules were resorbed. Cell lost water slightly and volume decreased slightly.

期间,细胞吸收了全部的毛基体、纤毛杆和微管结构,伴随着细胞剧烈的失水浓缩,细胞体积显著缩小;游仆虫在形成毛基体非吸收型包囊时,细胞仅经历了部分的脱分化过程,期间,细胞仅吸收了部分的毛基体及部分微管结构,并且细胞仅发生了轻微的脱水和浓缩,细胞以折叠的形式容纳在包囊壁内。

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采用不同浓度K2CrO4(0,0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

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