脯氨酸

The composition of amino in feed matter and feed draff after degradation for sixteen in rumen compared, from which we found the content of vary of amino acids in peanut meal and Soybean meal had increasing trends after sixteen hours cultured in rumen. The increasing trends of leucine, isoleucine, threonine, phenylalanine were greater. The increase trends of lysine, methionine, arginine and histidine were smaller. The content of varies of amino acid in cotton seed meal matter had decreasing trend s after sixteen hours cultured in rumen, but decreasing value was small. The compositions of amino acid in lees and rape seed meal matter were steady after sixteen hours cultured in rumen.

通过把饲料原料中氨基酸的组成和瘤胃降解16h后饲料残渣的氨基酸组成比较发现各种蛋白质饲料瘤胃降解前后氨基酸组成均发生了一定的变化，总的来说，氨基酸浓度在降解后有上升趋势(p＜0.05)的有亮氨酸、异亮氨酸、苯丙氨酸、缬氨酸、苏氨酸、丙氨酸和脯氨酸，氨基酸浓度在降解后有下降趋势(p＜0.05)的有赖氨酸、精氨酸、谷氨酸和胱氨酸，氨基酸浓度在降解后差异不显著的有组氨酸、天门冬氨酸、甘氨酸，降解后无明显规律的有蛋氨酸、丝氨酸和酪氨酸。

Development period of the parasitoid pupa from the 5th instar larva fed on MSB was significantly longer. Length of cocoon of MSB treatment was significantly shorter than the control. However, no obvious differences were detected in the development periods of egg to larva, cocoon mass size, wasp emergence rate, sex ratio, longevity and forewing length of wasp between KMD1 and the control. 3. Amino acid content in the haemolymph of SSB after fed with transgenic rice Although there were no changes on the components of amino acids in the haemolymph of SSB after fed with two transgenic rice lines, there were some changes on relative contents of amino acids.

取食KMD1 36h后0天，20种氨基酸中除天门冬酰胺、丝氨酸、苏氨酸和甲硫氨酸外，其它16种氨基酸的含量都高于对照，其中组氨酸、甘氨酸、苯丙氨酸、异亮氨酸、赖氨酸差异达显著水平，谷氨酰胺、脯氨酸差异达极显著水平，与氨基酸总量表现一致的变化趋势；取食KMD1后1天，除天门冬酰胺、谷氨酰胺和赖氨酸高于对照外，其它17种氨基酸均较对照低，但仅丝氨酸、苏氨酸和色氨酸与对照差异显著；取食KMD1后3天，绝大多数氨基酸含量的变化与取食KMD1后0天的趋势一致，除丙氨酸和色氨酸比对照稍低，其它18种氨基酸均高于对照，其中精氨酸、缬氨酸差异显著，丝氨酸差异达极显著。

Three antioxidative parameters(SOD super oxide dismutase、MDA malondialdehyde and GSH reduced glutathione hormone)were also measured.Results:The skin elasticity and water content improved increasingly according to the MPA measurements of the two group 4 weeks after the last irradiation.The hydroxyproline of the skin in the Q-switched 1064-nm Nd:YAG laser of 0.6J/cm~2、1.5 J/cm~2 and 2.5 J/cm~2 group showed 7.1%,13.1%and 19.8%(p＜0.05)increase respectively compared to their controls,the enhancement of dermal thickness were 15.5%,29.7%and 38.4%;The hydroxyproline of the skin in the Q-switched 532-nm Nd:YAG laser of 0.5J/cm~2、1.2 J/cm~2 and 2.0 J/cm~2 group showed 6.9%,9.6%and 14.2%(p＜0.05)increase respectively compared to their controls,the enhancement of dermal thickness were 5.5%,15.1%and 29.9%,among which the Q-switched 1064-nm laser group had the greatest effect. The result of antioxidative parameters showed some juvenescence change at the radiated skin.

结果：末次照射后第4周，Q开关1064-nmNd：YAG激光组(以下简称1064 nm组)和Q开关532-nmNd：YAG激光组(以下简称532-nm组)大鼠各实验侧的皮肤弹性和含水量都明显好于对照侧；1064-nm组和532-nm组实验侧的皮肤羟脯氨酸含量和真皮内胶原厚度都较对照组显著增大(P＜0.05)，其中1064-nm组的三种不同能量密度：0.6 J/cm~2、1.5 J/cm~2、2.5 J/cm~2的激光照射的皮肤区域的羟脯氨酸含量增加率为7.1%，13.1%和19.8%，真皮厚度增加率为15.5%，29.7%和38.4%，532-nm组的三种不同能量密度：0.5 J/cm~2、1.2 J/cm~2、2.0J/cm~2的激光照射的皮肤区域的羟脯氨酸含量增加率为6.9%，9.6%和14.2%，真皮层厚度增加率为5.5%，15.1%和29.9%，相比而言1064-nm组的羟脯氨酸及真皮层厚度增加率均较高；抗氧化指标显示两组大鼠实验侧皮肤的抗氧化能力增加，呈年轻化改变。

We also found that the content of Na(superscript +), K(superscript +) and proline in leaves varied with different treated days. Under all treatments, the absorption of Na(superscript +) increased and the content of proline in leaves decreased with the prolonging of treated days. The absorption of K(superscript +) measured on the 7th day and the 21st day increased and was restrained on the 14th day, which lead to the decline of K(superscript +)/Na(superscript +) ratio. While the content of praline measured on the 14th day remarkably increased with the increase of salinity. Our experiment indicated the live oak seems to adapt the salt stress by increasing the absorption of K(superscript +) during the earliest and late stages of salt stress and accumulating proline in leaves during the middle stage of salt stress.

同时发现，盐胁迫处理不同时期对叶片脯氨酸含量和Na、K含量的影响各不相同，在处理7d和21d时，叶片对Na和K的吸收量均有增加，使K/Na维持相对稳定的比值，而脯氨酸含量随NaCl质量质量浓度的增加减少；在处理第14d时，对Na吸收增加的同时，抑制了对K的吸收，导致K/Na比值随NaCl质量质量浓度的增加而迅速下降，而此时叶片脯氨酸含量随着盐质量质量浓度的增加而迅速增加，表明在盐胁迫早期和晚期，弗栎可能通过增加对K的吸收以减轻Na离子毒害效应，而在盐胁迫中期，叶片积累脯氨酸是适应盐胁迫的方式之。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明：(1)在膜透性的研究中，丛毛羊胡子草抗寒性最强，采用电导率法测得半致死温度LT_(50最低，丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃；在可溶性蛋白含量研究中，抗寒锻炼前后三种植物的可溶性蛋白含量均增加，同时采用SDS-PAGE法，通过对蛋白条带分析，以丛毛羊胡子草条带数最多且着色深；(2)在可溶性糖的研究中，三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高，且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势，丛毛羊胡子草可溶性糖含量下降较小；从游离脯氨酸含量可知，低温的胁迫影响了植物叶片游离脯氨酸的含量变化，植物的抗寒性与游离脯氨酸有关；低温胁迫下三种植物叶绿素含量随着温度的下降，叶绿素含量、叶绿素a、叶绿素b和叶绿素a／b均呈下降趋势，狗牙根对低温胁迫最为敏感；(3)低温锻炼前后，丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的，特别是过氧化物酶，说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

The result was also confirmed by measuring the transcriptional levels of four hordein homologous genes in thebarley cells. Free proline participates in the synthesis of B3-Hordein, which plays a key rolein regulating the balance of the cellular proline.

而大麦(PC1163)细胞醇溶蛋白4个同源基因的转录水平的检测结果也证实了这一点，游离脯氨酸参与合成B3-Hordein，在降低细胞内脯氨酸含量并维持脯氨酸动态平衡方面起了重要作用。

Neural networks based on local sequence and amino acid component with the best structures and linear equation are constructed. The results of testing to the networks indicate that the forming of cis-proline has distinct relationships with local sequence and amino acid component. A new method for peptidyl prolyl cis/trans conformation prediction based on the theory of support vector machines was introduced. The SVM represents a new approach to supervised pattern classification and has been successfully applied to a wide range of pattern recognition problems.

通过运用神经网络与线性拟合的方法，揭示了脯氨酸局部序列、肽链氨基酸组分对于顺式脯氨酸形成与否的影响，构造了基于局部序列和基于氨基酸组分的最优结构的神经网络，以及线性拟合方程，同时对顺式脯氨酸进行了筛选，结果表明，利用神经网络的方法可以提高顺式脯氨酸筛选时的信噪比，脯氨酸采取顺式还是反式构象与其局部的序列以及整条肽链的氨基酸组分均有较强的关联。

The results show that in peptide chain, the residue at the first position C-terminal influences the usage of proline synonymous codon greatly and proline synonymous codons contain some factors influencing the isomerization of the prolyl peptide bond.

研究结果表明：与脯氨酸相邻的C端第一个残基对脯氨酸同义密码子的使用有较大影响；脯氨酸同义密码子内携带有影响脯氨酸肽键构象的因素。

They were two new compounds, [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu),7],[2-N-(1-methoxycarbonylethyl)guanosine, 10], and eight known compounds,(p-hydroxybenzoic acid, 1),(methyl succinate, 2),(russulaceramide, 3),(phenylalanine, 4),(guanine, 5),(β-carboline, 6),(uridine, 8), and (adenosine, 9). All the compounds were isolated from Amanita exitialis for the first time.

分别为：对羟基苯甲酸(p-hydroxybenzoic acid，1)、丁二酸二甲酯(methyl succinate，2)、神经酰胺(russulaceramide，3)、苯丙氨酸（phenylalanine，4）、鸟嘌呤核苷(guanine，5)、β-咔啉(β-carboline，6)、环（脯氨酸-缬氨酸-苯丙氨酸-苯丙氨酸-脯氨酸-缬氨酸-苯丙氨酸-丝氨酸-亮氨酸） [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu)，7]、尿嘧啶核苷(uridine，8)、腺嘌呤核苷(adenosine，9)、2-N-(1-甲氧羰基乙基)鸟苷[2-N-(1-Methoxycarbonylethyl)guanosine， 10]。10个化合物均为首次从致命鹅膏中分离得到，其中化合物7和10为新化合物。

As one of the most important osmoregulation actor, firstly,Proline plays a keyrole in the osmotic potential adjustment in plant cells. Secondly, Proline can preventNaCl from damaging the structure of biomacromolecule in cells. Thirdly, the highdissolubility and non-rejection capability of Proline can enlarge the solution volumnof the cell, so the concentration of salt will be diminished in cytoplasm and the saltstress will be relieved. Forthly, the accumulation of Proline could prevent thedehydrolysis from sap cavity in cytoplasm. Fifthly, the synthesis of plant chlorophyllwill be diminished under the salt stress, but the Proline can provide request ofchlorophyll synthesis.

脯氨酸作为渗透调节剂对植物体细胞渗透势的变化起重要作用：一，在盐胁迫下，脯氨酸在植物体内是一种重要的渗透调节剂，它是一些高等植物和绿藻抗盐和抗旱的主要渗透剂之一；二，脯氨酸可以保护细胞中的生物大分子的结构，使之不被NaCl破坏，并能维持其完整的水合范围；三，脯氨酸的高度溶解性以及其对植物各种酶活性不抑制性，可以扩大细胞的溶解容积，从而降低细胞质液中盐的浓度，减轻盐的胁迫作用；四，细胞质中积累的大量脯氨酸可以防止液泡对细胞质的脱水作用；五，在盐胁迫下，植物叶绿素的合成受到抑制，叶绿素的合成需要脯氨酸。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。