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脓细胞素

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Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池,将lipid A包被于非衍生样品池,分别建立以PGN、CpG DNA及lipid A为靶点的技术平台,对114种抗炎中药水提物进行筛选、评价其活性物质含量,并评估出针对上述三种病原分子均具有较高结合活性的中药;(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术,从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分;在体外实验中,测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力;MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响;ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用;在体内实验中,采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌,建立细菌脓毒症小鼠模型,观察活性组分对脓毒症模型小鼠的保护作用。

推荐网络例句

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。