脓生成

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

PTX at tenuates the ALI induced by LPS or sepsis in rat. The therapeutic effects of PTX include:improve oxygenation, at tenuate pulmonary edema formation, enhance animal survival in sepsis-induced ALI. The mechanisms may related with the effects of PTX inhibiting the chemotaxis, migration, activation of inflammatory leukocytes, diminishing the production of active oxygen, and inhibiting inflammatory cytokine release partly.

4PTX对大鼠内毒素性ALI及脓毒症性ALI均有一定的防治作用，主要表现在改善动脉血氧合，减轻肺水肿形成，提高脓毒症性ALI大鼠的存活率等方面，其机理可能与抑制白细胞趋化与激活，减少肺内白细胞渗出及活性氧产生，一定程度上抑制炎性细胞因子生成等有关。

Six and twelve boors after sepsis, leptin levels in liver homogenate were detected by radioimmunoassay serum alanine aminotransferase and 4 enzymes in liver homogenate. myeloperoxidase, glutathin-S-transferase, xanthine oxidase and superoxide dismutase, all related with synthesis of free radicals, detoxication and purine metabolism, were detected by 96 well spectrophotometry. H-E staining was used to examine the histopathologic changes of liver.

于脓毒症后6 h和12 h，采用放射免疫法测量肝组织匀浆液中Leptin水平，采用96孔分光光度法检测血清丙氨酸氨基转移酶及髓过氧化物酶、谷胱甘肽－S转移酶、黄嘌呤氧化酶、超氧化物歧化酶等4种与自由基合成、解毒和嘌呤生成代谢相关的酶的水平，同时以H-E染色法观察肝组织病理学改变。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。