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The results were as follows: ET〓-RNA expression levels in hypothalamus, hippocampus and temporoparietal grey and white matters of the low and high speed injury groups were elevated significantly than that of control group (p<0.05), but in the above areas of brain, ET〓-RNA expression in hypothalamus and hippocampus were the highest (p<0.01), especially in the high speed injury group; ET〓 contents in hypothalamus and hippocampus in the two injury groups, especially in the high speed injury group, increased very significantly than that of control group (p< 0.01); ET〓 contents in CSF and plasma in the two injury groups, also especially in high speed injury group, increased markedly after 2 hours, and lasted for 8 hours after injury; ET〓 contents in the abovt areas of brain were of highly positive correlation with that in plasma or in CSF.

实验显示:致伤犬伤后海马、丘脑下部、颞顶叶灰、白质内皮素—1RNA表达较对照组显著增强(p<0.01,p<0.05),其中以海马和丘脑下部最显著(p<0.01),高速组最明显(p<0.01);海马和丘脑下部ET〓含量较对照组显著升高(p<0.01);血浆、CSF中ET〓含量在伤后2h即极显著升高(p<0.01),并持续至伤后8h,上述各组均以高速组最为显著(p<0.01);各脑区和血浆,CSF中ET〓含量间呈显著正相关。

The effect of the overlaying tiss ue on the measurement can not be ignored because the most interested tissue to b e monitored is the deeper tissue layers,such as muscle and brain,which are under adipose or skull.Apart from this,variations of the regional hemoglobin oxygen s aturation (rsO2),haematocrit and regional blood volume hav e their influences on the measurement.

然而在人体组织这个复杂的不均匀混合介质中,深层组织往往是感兴趣的待测部位,如皮脂下的肌肉组织,头皮和颅骨下的脑灰白质等,这时外层覆盖组织的存在会直接影响检测的精度;另外,全血的局部氧饱和度( rsO2)的波动,红细胞比容的变化,以及血容积都对测量精度产生影响。

Methods Using immunohistochemistry technology, we checked up the expression of CD44 and β amyloid protein in the cerebral cortexes, hippocampi and cerebellums of 11 cases of postmortem autopsy brain tissues.

结果发现CD44在11例脑内白质的部分星形胶质细胞上存在特异性表达,但在AD皮层的灰质也存在一些表达;AD组与ND组相比,CD44阳性度明显增加(P<0.01)。

The strength of this study arises from the fact that all the patients had repeated neuroimaging documenting recovery, ensuring the diagnosis of RPLS and excluding mimickers.

鉴于患者通过复查神经影像学发现病灶消失可确定可逆性后部白质脑病综合征的诊断并排除其它的鉴别诊断这一事实,本研究显得尤为重要。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

The scale examined the severity of white-matter changes on 3 serial CT slices and graded separately for the 2 distinct regions anterior and posterior to the central sulcus: 0 = no lesion, 1 = partly involving the white matter, and 2 = extending up to the cortex.

rt-PA溶栓治疗和对照组比较发现严重白质脑病的患者(vSS=3-4分)并没有影响治疗的效果,rt-PA溶栓组mRS评分为0-1分的患者所占比例为31.6%而对照组为14.7%。

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