脑损伤

(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论：(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变，针刺可以促进肢体功能恢复；(2)脑缺损伤区可见PCNA阳性细胞，存在细胞再生现象，针刺可以增强损伤区PCNA的表达，有利于损伤的修复和功能重建；(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组，随着损伤逐渐得到修复，细胞增殖现象减弱；(4)脑缺血损伤区可见VEGF阳性细胞，存在内皮型细胞再生现象，针刺可以增强损伤区VEGF的表达，有利于脑损伤后保护和缺血区血管再生；(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组，随着缺血损伤的修复，缺血缺氧状态得到改善，产生的VEGF减少；(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞，前者是增殖细胞的标志，后者是促进血管再生的重要因子，可以推断，脑缺血再灌注损伤后脑内存在血管再生现象，针刺可以促进损伤区的血管再生，更迅速而充分的恢复损伤区的血供，促进脑损伤区的修复；(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低，说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

Results (1) The water content and glutamate in the injured brain were significantly greater than those in the control group.(2) In the injured brain, the contents of water and glutamate showed a self-diffusion process.(3) The contents of water and glutamate in the LTG-treated group were significantly lower than those in the nontreated group.

结果 (1)脑损伤组脑组织的水含量，细胞外液谷氨酸浓度明显高于对照组；(2)脑损伤组损伤区域的脑水肿和细胞外液谷氨酸呈自身扩播性；(3)拉莫三嗪治疗组脑组织含水量以及谷氨酸浓度明显低于脑损伤非治疗组。

objective:to study the changes of dynamic electrocardiogram in patients with brain injury.methods:fifty-six patients with acute brain injury were recruited in this study.the glasgow coma scale, the initial computed tomogram and ambulatory electrocardiogram were performed in all subject.data were analyzed according to different place and severity of brain injury.results:ecg abnormalities were found in 32 patients out of 56 patients with acute head injury(57.14%).

目的：观察脑损伤患者的动态心电图改变，了解急性脑损伤致心电异常的特点及其与损伤部位和严重程度的关系。方法：选取我院急性脑损伤患者56例，经glasgow评分分型，脑ct确定损伤部位，并行24h动态心电图检查，观察急性脑损伤后心电异常的发生情况，并根据脑损伤部位和严重程度分组观察，以了解其心电异常的特点。

The Glasgow coma scale, the initial computed tomogram and ambulatory electrocardiogram were performed in all subject.Data were analyzed according to different place and severity of brain injury.Results:ECG abnormalities were found in 32 patients out of 56 patients with acute head injury(57.14%). ST-T abnormities were found in half of the brain injury patients.

选取我院急性脑损伤患者56例，经Glasgow评分分型，脑CT确定损伤部位，并行24h动态心电图检查，观察急性脑损伤后心电异常的发生情况，并根据脑损伤部位和严重程度分组观察，以了解其心电异常的特点。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

So far, the convincible mechanism involved in the development and progress of the inflammatory responses after SCI is not quite clear.

在脑损伤的研究中发现，脑损伤后多种促炎性细胞因子、趋化因子明显上调，加剧损伤后炎症反应，在继发性脑损伤的病理过程中起重要作用。

To investigate the protective effect of anti-endothelin-1 antibody on neurogenetic pulmonary edema,the anti-endothelin-1 antibody was used in the rats which underwent brain injury referring to Marmarous′model.

采用Marmarous脑损伤模型致大鼠重度、弥漫性脑损伤，观察内皮素-1（ET-1）单克隆抗体对弥漫性脑损伤大鼠肺泡隔面积密度及肺水含量的影响，同时用光镜和电镜观察大鼠肺组织形态学改变。

Results Serum EDLS increased within 24 h after brain trauma of different degree in all patients with brain injuries of various intensities.The group of slight injury was the most typal,EDLS demonstrated no change or decrease in a few patients with the injury intensity growing;In most cases EDLS decreased after brain operation or accompaning the wounded recovery; Contents of EDLS at 24 h among different pathological types of brain injuries showed no difference, but in the successful recovery patients with severe or ultra-severe brain injuries, EDLS contents at 72 h posttrauma were lower than in the patients of death or disability. The above change characteristics of EDLS related to injury locations and the degree of brain injury.

结果 不同程度脑外伤后24小时内血清EDLS均升高，以轻伤组最为典型；随着损伤程度的加剧，少数病例EDLS不变甚至下降；术后或随伤情的恢复，EDLS多同步下降；不同病理类型的脑损伤24小时内EDLS无明显差异，在成功康复的重型、特重型脑损伤病人，伤后72小时EDLS含量低于死亡或伤残组；EDLS 的上述变化特点与损伤部位和程度不同有关。

Every group rats were divided into two parts with the same rats. At last the rats were redivided ,then them were killed and their brains were excluded after one hour.4 hours or one week.Results PO2 and cerebral blood flow of E-selectin 2mg/kg group were higher than that of saline and lower than that of l0mg/kggroup(p.01).The volume of cerebral infarct and blood speed of E-selectin 2mg/kg group was smaller than that of saline and larger than that of l0mg/kg group(p.01).At the same time PCh.speeds.flows and the volume of infarct were related with the time (p.01).Conclusion E-selectin can effectively increase PC2 and rCBF after ischemia and reperfusion .reducing infarct volumes and speeds of blood at the same time . Artificial synthetic E-selectin can effectively reduce the reperfusion injury following cerebral ischemia.

结果 生理盐水组，2mg／kg组，10mg／kg组PO_2值依次提高(p＜0.01)，脑皮质血流量依次提高(p＜0.01)；脑血流速度依次减低；脑梗死体积依次减少(p＜0.01)；同时在测定的时间段里PO_2和脑组织血流量值与时间成正相关(p＜0.01)；脑梗死体积和脑组织血流速度值与时间成正相关(p＜0.01)。E一选择素在脑缺血再灌注损伤中表达的实验研究中文摘要结论E一选择素能增加脑缺血再灌注损伤的脑组织氧饱和度，脑血流量，减低脑血流速度；减少脑损伤体积；从而保护了脑缺血再灌注损伤的脑组织。

EXPERIMENT 1 Objective To study the regularity of the injury of the mice learning and memory ability, after the whole brain irradiation. Method After anaesthetized with thiopentone, the whole brain of Kunming mice was irradiated with 10MeV electron beam. Mouse learning and memory ability were measured with step down method on the first, second, third, fourth, fifth, fourteenth, twenty-eighth, fifty-sixth and eighty-fourth day after the irradiation.

随着现代放疗技术及计算机、分子生物学等相关学科的快速发展，肿瘤疗效逐步提高，脑等晚反应组织的放射损伤越来越受重视，因而有必要采用动物实验及分子病理学手段，深入研究放射性脑损伤的发病机理和早期症状，以及活血化瘀中药的防治作用，为此我们设计并完成了学习记忆力放射损伤规律、脑室管膜下区细胞凋亡及相关基因表达、中药防治放射性脑损伤及分子机理三个实验，介绍如下：实验一目的研究照射后鼠学习记忆力损伤变化规律。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。