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Apgar Point System for Neonatus was used as thejudgement standard of neonatal upshot.

结果:FST段改变,FQRS时限延长>0.055,应注意胎儿宫内窘迫或脐带绕颈。

After allowing 1 day for adherence to culture flask, nonadherent cells were removed.

三、脐带血造血前体细胞在间充质干细胞微环境中的增殖动力学研究。

The optimal yield of MAPCs was obtained when mononuclear cells were cultured at a initial plating density of 1 × 10~6 cells / cm~2 and the medium was exchanged to remove the nonadherent cells after 72 hours of inoculation.

结果表明,在低血清条件下,DMEM/F12培养基更适合于脐带血MAPC生长;1×10~6/cm~2是原代培养的适宜接种密度,原代第72小时首次换液为最佳换液时问。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法:穿刺抽取15 mL脐带血,密度梯度法分离吸取分层界面的白色环形絮状物,其中富含单个核细胞,离心后沉积细胞用含15%胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮,调整细胞密度为1×109 L-1接种于25 T培养瓶内,放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养,72 h后更换培养基,去除红细胞及其他未贴壁细胞,至70%~80%融合时传代。

Methods 1 860 cases of pregnant women between gestational ages of 37~ 42 weeks were studied 1~ 2 weeks before delivery by CDFI, nonstress testing and ultrasonography to monitor umbilical cord entanglement.

方法对 186 0例孕周为 37~ 42周的孕妇于产前 1~ 2周内联合应用CDFI、NST及B超进行监测,筛查胎儿有无脐带缠绕。

Objective To explore the value of Type O-dip wave or V-spike wave during nonstress testingNST)for diagnosis of umbilical cord entanglement and parturition disposal.

目的 探讨胎儿监护无负荷试验中Type O-dip波或V型棘波对脐带缠绕的诊断及分娩处理中的价值。

Objective To explore the predictive value of combined application of umbilical artery color Doppler flow monitoring, nonstress testing and ultrasonography in monitoring umbilical cord entanglement.

目的 探讨彩色多普勒脐动脉血流仪、无负荷试验及B超检查 3种方法联合使用,对产前脐带缠绕的预测价值。

O.'PR1{ M Objective To explore the value of Type O-dip wave or V-spike wave during nonstress testingNST)for diagnosis of umbilical cord entanglement and parturition disposal.

目的 探讨胎儿监护无负荷试验中Type O-dip波或V型棘波对脐带缠绕的诊断及分娩处理中的价值。

Results The Umbilical cord factor and oligohydramnios is a main reason, have 31.96% and 30.33% respectively.

结果 脐带因素、羊水过少为主要原因,分别占31.96%,30.33%。

Human umbilical cord perivascular cells:a source of mesenchymal progenitors[J].

脐带Wharton胶间质干细胞向软骨细胞诱导的实验研究[J]。

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