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Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后,骨髓间充质干细胞多为成纤维样细胞,仅有少量内皮样细胞;而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外,还有较多的内皮样细胞和单核-巨噬样细胞。

Gentle fundal massage and gentle traction on the cord facilitates delivery of the placenta.

轻柔地按压宫底和牵引脐带有助于胎盘娩出。

Clinical Efficiency of Meningococcus Group A Capsular Polysaccharide Vaccine in Children Three Months to Five Years of Age Cellular and Humoral Components of Monocyte and Neutrophil Chemotaxis in Cord Blood Oral non-absorbed Antibiotics Prevent Infection in Acute non-lymphoblastic Leukemia Aspirin-induced Hepatotoxicity and Its Effect on Juvenile Rheumatoid Arthritis Prediction of Morbidity in Hemophilus Influenzae Meningitis Neurological Sequelae in Children Surviving Mechanical Ventilation in the Neonatal Period Developmental Effects of Prolonged Pregnancy and the Postmaturity Syndrome Long-term Outcome of Hemophilus Influenzae Meningitis Related to Antibiotic Treatment Recommendations for a National Policy on Poliomyelitis Vaccination Impaired Humoral Immunity in Treated Hodgkin's Disease Development of Immune Response during Typhoid Fever in Man In Vitro Susceptibility of Recently Isolated gram-negative Bacteria to Gentamicin, Sisomicin, Tobramycin, and Amikacin Studies of Urticaria and Acute Serum Sickness with the CI Precipitin Test Epidemic Measles in a Highly Vaccinated Population Congenital Chloride Diarrhea Acute Parotitis Associated with Influenza, Type A: Twelve Cases Urinary Tract Infection in high-risk Newborn Infants Maternal ABO Blood Group Type B: A Risk Factor in the Development of Neonatal Group B Streptococcal Disease

脑膜炎双球菌A羣被囊多醣类疫苗接种在3个月到5岁大小孩之临床效果脐带血内单核球及嗜中性球化学向性之细胞成分及体液成分口服不被吸收的抗生素可预防性急非淋巴球性白血症病人罹患感染阿斯匹灵引起之肝毒性及其对幼年性风湿样关节炎之影响流行感冒杆菌脑膜炎预后之预测新生儿时期接受人工呼吸器其日后神经系统上之后遗症过期妊娠及过度成熟徵候对发育之影响流行感冒杆菌脑膜炎之长期预后与抗生素治疗之关系对&小儿麻痹预防接种的国家政策&的建议 Hodgkin's disease治疗后体液免疫之缺损伤寒患者免疫反应的研究目前分离出来的革兰氏阴性菌在体外对Gentamicin,Sisomicin,Tobramycin和Amikacin之感受性荨麻疹和急性血清病CI沉淀素试验的研究在大多数接种人羣发生的麻疹流行先天性氯腹泻与influenza A型病毒有关的急性腮腺炎:12病例报告高危险性新生儿的尿道感染母亲血型B型:为新生儿发生B羣链球菌疾病之个危险因素

The autologous cord blood serum method could effectively culture mesenchymal stem cells with hepatogenic differentiation ability.

采用自体脐血清有效地培养脐带间充质干细胞,具备向肝细胞的分化能力。

Methods Autologous cord blood serum was used to culture cord mesenchymal stem cells. An enzyme digesting method was used to isolate mesenchymal stem cells from the cord. Morphology, immunotyping and hepatogenic differentiation of these cells were assayed.

分离自体脐血清用于后续细胞培养,利用酶消化法从脐带组织中分离培养间充质干细胞,检测其形态和免疫表型,并采用两步法诱导其向肝细胞诱导分化。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子,我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异,发现ZF(HCF-binding transcription factor,Zhangfei)和SH3GLB1(SH3-domain GRB2-like endophilin B1)在这三种不同来源的红细胞内具有显著的表达差异,并通过实时定量PCR方法验证了差异的真实性。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法,从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干/祖细胞的单个核细胞(Mono-Nuclear Cell,MNC),进行初步纯化及体外扩增后,组合使用Epo、IL-3、GM-CSF等细胞因子,将造血干/祖细胞向红系方向进行诱导培养。

GRP can also make mice's MNC and hematopoietic stem cells from human's umbilical cord blood amplification. We use colony formation to analysis the linear relation during the cells'growth and the medicine's dosage, examine the amplification of CFU-GM induced by hypothallus and its supernatant, RT-PCR is used again to check three cytokines'genes.

发现GRP对小鼠MNC扩增有作用,从而将GRP运用到人脐带血MNC的扩增分化上,用集落形成法分析药物与扩增的线性关系,基质层、基质上清对CFU-GM的影响,用RT-PCR技术检测基质层细胞生长因子基因的扩增。

Methods To select who lived in iodine sufficient area which was taken salt iodization program to regard as research target in the assignment for study.

选择采取食盐加碘干预措施后当地居民碘营养充足地区的孕妇作为的研究对象,观察指标有孕妇尿碘、新生儿脐带血TSH和新生儿足跟血TSH水平以及相关因素。

In the next most serious type, the coverings of the cord pass back through the opening to form a cyst-like swelling called a meningocele.

在未来的最严重的类型,覆盖脐带血通过回到通过开放,形成囊肿样肿胀,称为脊膜膨出。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。