脐带

Objective: To study the long-term effect of cord blood infusion to chronic aplastic anemia.

目的：观察脐带血治疗慢性再生障碍性贫血的远期疗效。

There was a soft lump right on my belly button,it had gotten more and more bigger,and it had become ashine and nigrescent.My master took me to the vet,and the vet said it was a disease so called"here-near"(VV's master notes:maybe it is the same as exomphalos).The vet suggested that we'd better have a surgery to remove the lump,he told us it was a very small surgery and we needn't worry about it at all.

我肚脐眼上有个小软包，最近越来越大了，并且有点发亮发黑，主人带我去看兽医，兽医说是一种叫&赫尔尼亚&的病，原因是刚出生的时候脐带没有扎好，导致肠子里的小东西不断掉下来，堆积在肚脐眼上结成了块，现在需要动手术拿掉它，兽医说这是一个很小的手术，告诉我们不用担心。

OBJECTIVE: To compare biological feature of BMSCs by the umbilical cord serum and adult autoserum in vitro.

目的：比较脐带血清和成人自体血清体外培养人骨髓间充质干细胞的生物学特性。

Purity and differentiated potency of BMSCs in umbilical cord blood serum are better than those in the adult autoserum in vitro.

脐带血清组培养的骨髓间充质干细胞的纯度和体外诱导分化能力比成人自体血清组高，更适合临床应用。

METHODS: BMSCs were obtained from 16 fresh adult bone marrow by gradient centrifugation with Ficoll Paque, cultured with DMEM/F12 with 10% umbilical cord blood serum and 10% adult autoserum. The fourth passage was used in this study. The surface antigens of BMSCs were detected by flow cytometry.

采用Ficoll Paque细胞分离液从16例新鲜成人骨髓穿刺液中分离骨髓间充质干细胞，分别用含体积分数为10%脐带血清、成人自体血清的DMEM/F12培养基培养，取第4代细胞进行实验。

Methods Eight bigeminal pregnant ewes in latter 2nd-trimester and 3rd-trimester were taken into the research of fetal cardiac intervention. Under continuous ultrasound guidance, advanced an cannula and stylet needle through the maternal abdomen, uterine wall, and fetal lamb chest wall and into the fetal LV, then imitate human balloon aortic valvuloplasty at valve ring level.

以8只中后期双胎妊娠黑山羊为研究对象，在超声引导下经皮/子宫穿刺胎羊左心室，并在主动脉瓣水平模拟人类主动脉瓣球囊扩张术，从而建立胎儿宫内心脏介入手术动物模型，从动物准备、术中胎羊体位、穿刺部位选择、介入技术要领、超声使用、胎羊保温、胎盘脐带保护等方面进行探讨总结。

Key words: umbilical cord blood;mesenchymal stem cells; chondroblast; transforming growth factor-β1

脐带血；间充质干细胞；成软骨细胞；转化生长因子-β1

Objective To explore whether mesenchymal stem cells could be isolated from human umbilical cord blood and would be able to differentiate into chondroblast in vitro.

目的：探讨人脐带血间充质干细胞存在及体外向成软骨细胞分化的可能性。

Although PU was suitable for cell seeding than other substrata such as FEP, PTFE because of its more hydrophilic surface, bare PU was not good for cell colonization because cells are incapable of confluently proliferating or strongly adhering on the surface.

通过表面光化学接枝、表面生物活性因子固定化分别将可促进细胞粘附和生长的功能性单体和细胞生长因子引入到PU表面，并研究了改性PU膜对人体脐带静脉内皮细胞相容性的影响。

Alpha-fetoproteinhas been separated and purified from 3～5 months embryon by using salting out, gel chromatography and affinity chromatography.

甲胎蛋白（Alpha-fetoprotein，AFP）可从胎儿组织、胎儿心血、胎儿肝脏、羊膜液、脐带血清、肝癌患者血清和腹水等多种材料中分离获得，分离纯化方法国内外已有不少报道［1～6］。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失