脊髓的

The CNTF and FGF2 positive cell number and the positive area percentage, as well as the number of neuron and neuroglia calculated by immunofluorescence analysis in the spinal cord.

将嗅鞘细胞移植入脊髓半横断SD大鼠，观察术后7天和21天嗅鞘细胞在脊髓内的存活情况，并对脊髓组织切片进行CNTF和FGF2免疫荧光组织化学染色，计数阳性细胞并计算阳性面积百分比。

Compared with the previous experiment model, this model which adopts the pressure Saccule to create gradually the pressure on the cervical spinal cord has its own advantages as follows: simple to operate, safe to carry out, successful rate is higher; cheaper catheter to obtain, oppressing the machine creation the convenience; to achieve the purpose that gradually pressure on the ventral lateral spinal cord, the medical screenage and electric physical examination reflect accurately the degree of the chronic pressure on the spinal cord; the change of Pathologic histology with the impediment on spinal cord function is similar to in the characteristic changes that of human CSM. ideally simulated the pathological procedure of the gradual chronic pressure in the course of the breakout of the CSM.

与既往实验模型比较，此模型采用压力球囊法致慢性颈脊髓压迫具有以下优点：手术操作简单易行，安全性及成功率较高；导管廉价，压迫器制作方便；达到了渐进性压迫的目的；影像学及电生理检测比较准确地反映了慢性颈脊髓压迫的程度；脊髓功能障碍后脊髓病理组织学改变类似于人类脊髓型颈椎病的特征性改变；较理想地模拟CSM发病中的慢性渐进性压迫的病理过程。

Syringomyelia is a condition where a fluid filled sac develops in the spinal cord.

脊髓空洞症是一种脊髓病变，脊髓内形成包含液体的囊。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Methods:64 cases of intraspinal spacc-occupying discase were examined by conventional myelography,CT myelography and MRI,CTM were performed after 6 hours of myelography,MRI were performed with SE sequence and combined with GE sequence.

脊髓造影采用常规脊髓造影和 CTM相结合。CTM在常规脊髓造影后 4～ 6 h进行。 MRI采用 SE序列加 GE序列。椎管内占位性病变的病灶检出率、定位和定性准确率做一统计和分析。

JiaJi electropuncture can enhance NTFS secret and promote endogene regeneration .It verifies that there is a new treatment time by JiaJi electropuncture to SCI.The research provides theoretical and experimental function for clinical treatment to SCI.

本实验从分子水平阐述了夹脊电针治疗脊髓损伤的积极作用，能够促进神经营养因子表达，抑制BAX蛋白表达，促进受损脊髓神经再生，证实了夹脊电针治疗脊髓损伤的有效性，本实验为临床治疗脊髓损伤提供了理论基础。

Result Compared to concurrent control groups, neuromotor function are significantly improved in 1h and 6h experiment group. The indexes of neural cell apoptosis decreased significantly (P 0.01). But in 24h experiment group, the decrease of the index are not statistically significant. Conclusion Early application of rhEPO could lessen the lesion of neuromotor function. The protection is partially due to the reduction of neural cell apoptosis.

结果：与对照组相比，脊髓损伤后促红细胞生成素1小时，6小时实验组神经运动功能明显改善，脊髓神经细胞凋亡指数均明显下降(P 0.01)；而术后24小时组与对照组相比，脊髓神经细胞凋亡指数的下降无统计学意义结论：早期应用外源性EPO对脊髓不完全损伤后引起的神经运动功能损害具有保护作用，此种保护作用可能与EPO能够减少神经细胞凋亡有关。

Increased expression of PENK mRNA was more marked in the ipsilateral dorsal horn of the spinal cord, especially in lamina Ⅲ-Ⅳ and the contralateral medulla, especially in the gigantocellular reticular nucleus, lateral paragigantocellar reticular nucleus and lateral reticular nucleus.

本实验用原位杂交技术对电针刺激后前脑啡肽mRNA在脊髓和延髓的表达进行了观察，发现给予大鼠单侧后肢电针刺激引起脊髓和延髓中PENK mRNA阳性神经元数显著增加，尤其是同侧脊髓背角各层PENK mRNA的表达，和对侧延髓网状结构如网状巨细胞核α部、网状巨细胞外侧旁核及外侧网状核中的PENK mRNA表达增加，这与脊髓上传的信号由同侧脊髓交叉到对侧脑干的走行方式是一致的。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation onthe 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF的mRNA表达上升，损伤8天到达高峰，此后SDNSF的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的mRNA阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果：HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化，膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变；Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少；免疫组化结果表明3组均有不同程度的caspase-3表达，损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01)，损伤上段及下段caspase-3表达较损伤节段为少(P.01)，但均高于正常对照组(P.01)。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失