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In the normal rats (n=5、6), CGRP-LI were mainly present in small and some medium-sized neurons and the primary afferent fibers and ter- minals in laminae Ⅰ,Ⅱ,Ⅴ,Ⅹ and Lissauer's tract of the spinal cord; GAL- and SP-LI were mainly present in small neurons and the superficial layers of the dorsal horn. After the adjuvant injection both CGRP-and SP-LI were in- creased corresponding to the development of arthritis. On day 2, CGRP-and SP-LI were moderately increased in laminae Ⅰ,Ⅱ and the posterior commis- sure of the spinal cord and small neurons in DRG, while SP was obviously in- creased in the superficial layers and DRG. On day 14, CGRP-LI were marked- ly enhanced in the above regions with the development of polyarthritis. Some- times many strongly stained long fibers could be seen in the posteriot commis- sure.

在DRG中,CGRP主要分布于小和中等神经元之中,SP和GAL主要分布于小神经元之中;2、注入弗氏完全佐剂(含冻干结核菌0.5mg)后第2天动物(各5~6例)产生具有明显红、肿、热、痛和痛敏的急性关节炎,免疫组化实验观察到注射侧脊髓背角和DRG中CGRP-和SP-LI明显增加;在佐剂后第14天,动物患肢肿胀、痛敏和自发痛均进一步加重,部分动物健肢也出现红肿,产生多发性关节炎时,脊髓背角浅层和DRG中CGRP-和SP-LI增加更为明显,并且在灰质后联合中可见许多深染的CGRP-LI长纤维;在佐剂后第21和28天,随着佐剂性关节炎的逐渐恢复,脊髓背角和DRG中CGRP-和SP-LI也接近正常。

The pathways that regulate tone are similar to those that regulate voluntary and involuntary motor movements and, as a final common pathway, involve the spinal reflex arc.

控制张力的传导通路和那些控制随意或非随意运动的通路是一样的,即脊髓反射弧,这是一个最后的公共通路:控制肌纤维的α运动神经元位于脊髓前角,发出反射弧的传出纤维,源自肌梭的传入冲动经由Ⅰa纤维传向脊髓,这些直接与α运动神经元连接的轴突中的一部分激活主动肌。

Syringomyelia is a condition where a fluid filled sac develops in the spinal cord.

脊髓空洞症是一种脊髓病变,脊髓内形成包含液体的囊。

Especially, the number of type Ⅰ complex terminals which come from unmyelinated afferent fibers recovered to the level of unoperated side. It indicates that morphine may promote the collateral sprouting and synaptic reinnervation of primary afferent fiber, unmyelinated afferent in particular. 4. Although the collateral sprouting and synaptic reinnervation were shown also in spinal cord of operated side in 34 days spared root rats, they were slower than that of morphine spared root group.

表明吗啡促进了备用根初级传入纤维,特别是无髓纤维更快地向L〓脊髓段侧支出芽,稍后在L〓平面脊髓内侧支出芽以及突触重建。4、34天备用根大鼠术侧脊髓内也有侧支出芽和突触重建,不过侧支出芽的速度要比吗啡组慢。5、电镜定量结果也显示,吗啡备用根大鼠和备用根大鼠术侧备用根的无髓纤维数比备用根大鼠非手术侧的分别增多60%和42%。

Objective: To explore the changes on expression of NT-4 and IGF-I in spared dorsal root ganglia (DRG, L6) in operated/acupuncture side and unoperated /unacupuncture side, spinal lamina II (L3, L5, L6) and Clarke' nucleus (L3) of normal adult, partial dorsal rhizotomy and acupuncture spared DRG cats so as to know the relation between NT-4, IGF-I and the plasticity of spinal cord as well as acupuncture promoting spinal cord plasticity.

目的:探讨NT-4和IGF-I在正常猫、部分去背根猫及针刺部分去背根猫的L_6背根节和脊髓Ⅱ板层(L_3、L_5、L_6)与背核(L_3)表达的时空变化,以了解该两因子在脊髓可塑性及针刺促进脊髓可塑性中的关系。

In pathological examination, the range of vacuolation and the inflammatory reaction were more lightly than that in Group B, and the number of normal neurocyte was higher than that in Group B.

组织病理学检查发现A组于脊髓再灌注后各时相点脊髓神经细胞空泡变性范围、炎症反应明显轻于B组,脊髓前角正常神经元计数均显著高于B组

Methods:64 cases of intraspinal spacc-occupying discase were examined by conventional myelography,CT myelography and MRI,CTM were performed after 6 hours of myelography,MRI were performed with SE sequence and combined with GE sequence.

脊髓造影采用常规脊髓造影和 CTM相结合。CTM在常规脊髓造影后 4~ 6 h进行。 MRI采用 SE序列加 GE序列。椎管内占位性病变的病灶检出率、定位和定性准确率做一统计和分析。

ERK1/2 immunohistochemistry positive staining coloration in cornu dorsale medullae spinalis of rats was Buffy color.Positive cells distributed in gray nucleus that nerve cell concentrated,and deep staining region localized at the nucelus of neurocyte.

针刺前后脊髓未见明显形态学改变。2、光镜下观察,大鼠脊髓背角ERK1/2免疫组化反应显色呈棕黄色,阳性细胞分布于脊髓灰质神经元集中的部位,针刺后深染部位向胞核集中。

Spinal cord injury is defined as disfunction of spinal cord which includes disfunction of motion,senseness,sphincter and reflection below injury face as the result of directed and indirected violence on normal spinal and spinal cord.

脊髓损伤指因直接暴力或间接暴力作用在正常脊柱和脊髓组织,致损伤平面以下脊髓各项功能包括运动、感觉、括约肌和反射功能障碍的疾病。

Results: During the development of spinal cord. NIDD mRNA was highly expressed at embryo 16 d and at postnatal 1 d, and co-localized with nNOS in the undifferenti ated ventral horn, as well in white matter. And then it decreased significantly till postnatal 12w. nNOS mRNA was highly expressed from postnatal 1 d to 3 d. The high expression of NIDD mRNA appeared at the 8 hour after spinal cord injury, and co-localized with nNOS in neurons of ventral horn, intermedial zone, the area around central canal and dorsal horn, and recovered to the normal at dry 7 day after SCI.

结果:大鼠发育过程中,胚胎16 d的大鼠脊髓中可见NIDD mRNA的高表达,在生后1d,与nNOS共表达于尚未分化成熟的前角,在白质也见NIDD的阳性信号成年后呈低表达;nNOS mRNA于生后1~3 d出现表达高峰;脊髓损伤后NIDD mRNA表达明显增多,在8 h到达高峰,分布于脊髓前角,中间带、中央管周围及后角nNOS阳性的神经元,7 d恢复至正常水平;nNOS mRNA在损伤后8 h达到高峰,1 d降低至正常水平。

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