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Results:The activity of ALT and AST of ASCI patients is much higher than that at recovery phase,and it is also higher than that of patients with spinal column fracture,while activity of patients with complete spinal cord injury is higher than that of patients suffered from incomplete spinal cord injury.

结果:急性脊柱脊髓损伤患者血清中ALT和AST活性显著高于其恢复期及单纯脊柱骨折患者,并且在急性期脊髓完全损伤患者血清中ALT和AST活性明显高于脊髓不完全损伤患者。

Results: The content of MDA, the expression of Caspase-3 and Bcl-xl were significantly increased in the ASCI control group as compared with the false-operation group.

80只SD大鼠,随机分为假手术组(n=16),ASCI盐水组(n-32)和ASCI治疗组(n-32),采用改良的Allen法,制成中度脊髓损伤大鼠模型;各组按术后3h、6h、24h、48h随机分为4个亚组;检测各亚组脊髓组织MDA的含量;以免疫组化检测各亚组脊髓组织Caspase-3表达和Bcl-xl基因表达。

JiaJi electropuncture can enhance NTFS secret and promote endogene regeneration .It verifies that there is a new treatment time by JiaJi electropuncture to SCI.The research provides theoretical and experimental function for clinical treatment to SCI.

本实验从分子水平阐述了夹脊电针治疗脊髓损伤的积极作用,能够促进神经营养因子表达,抑制BAX蛋白表达,促进受损脊髓神经再生,证实了夹脊电针治疗脊髓损伤的有效性,本实验为临床治疗脊髓损伤提供了理论基础。

Alanine, glycine and glutamine, and present weak resonances of creatinine also; diabetes mellitus patients show hyperglycorrhachia and strong resonances for ketone bodies and glycine; patients with benign prostatic hypertrophy or urethral stone have high levels of creatinine; the genital tumor females can be distinguished with low pyruvate levels on the bases of /sup 1/H-NMR spectra.

以下乃是由上述样品族群之氢光谱所得之结论;孕妇的脑脊髓液具有低浓度的脊髓糖;胺基酸(如丙氨酸;麸氨酸和甘氨酸)和肌酸酐;有酮体生成,糖尿病患的脊髓液在氢核光谱显示高浓度的脊髓糖,另外有丙酮酸;乙醯乙酸盐和甘氨酸的强烈共振讯号,而具摄护腺肥大症或是尿道结石的病患,具有高浓度的肌酸酐在其CSF的氢光谱中。

Result Compared to concurrent control groups, neuromotor function are significantly improved in 1h and 6h experiment group. The indexes of neural cell apoptosis decreased significantly (P 0.01). But in 24h experiment group, the decrease of the index are not statistically significant. Conclusion Early application of rhEPO could lessen the lesion of neuromotor function. The protection is partially due to the reduction of neural cell apoptosis.

结果:与对照组相比,脊髓损伤后促红细胞生成素1小时,6小时实验组神经运动功能明显改善,脊髓神经细胞凋亡指数均明显下降(P 0.01);而术后24小时组与对照组相比,脊髓神经细胞凋亡指数的下降无统计学意义结论:早期应用外源性EPO对脊髓不完全损伤后引起的神经运动功能损害具有保护作用,此种保护作用可能与EPO能够减少神经细胞凋亡有关。

MRI in CSM group, however, showed different degrees of disc herniation and formation of osteophyma with membranous sac and spinal cord compression.

结果 非CSM者MRI表现出椎间盘变性或轻度突出、骨赘形成但不构成对脊髓压迫;CSM病例表现不同程度椎间盘突出、椎体后缘骨赘形成及硬膜囊和脊髓受压,其中69例(86.25%)脊髓受压,11例(13.75%)脊髓受压变性。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果:HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化,膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变;Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少;免疫组化结果表明3组均有不同程度的caspase-3表达,损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01),损伤上段及下段caspase-3表达较损伤节段为少(P.01),但均高于正常对照组(P.01)。

Result Among 10 cases, 5 were characteristic by spinal cord disorder and misdiagnosed as cevial spondylosis, myelitis, compressive myelopathy or spinal radiculitis;4 by hemiplegia and were misdiagnosed as stroke, and 1 by optic nerve syndrome and were misdiagnosed as vitreum turbid, p...

结果 5例以脊髓症状起病,被误诊为颈椎病、脊髓炎、脊髓压迫症、脊髓神经根炎;4例以偏瘫、偏侧感觉障碍起病,被误诊为脑卒中;1例以视神经症状起病,被误诊为玻璃体浑浊、视乳头水肿。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标:应用光学显微镜、电子显微镜观察脊髓形态的变化,采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌,以改良的Gale联合行为评分法对脊髓功能进行评定,结果:免疫组织化学检测显示,嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度,延缓白质神经纤维的减少,促进髓鞘的修复与再生。

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