脊髓

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

Methods Forty rats with spinal cord injury were made with Allen weight drop and spinal cord tissues were removd from 1 h to 4w after injury.

采用原位末端标记法，对大鼠脊髓损伤不同时间脊髓组织中凋亡细胞的数目和部位进行观察和分析，并作正常对照。

Eighty SD rats were randomly divided into four groups: acute spinal cord injury groups ( SCI groups, n=20);administration of Ulinastatin (U groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour.;administration of Methylprednisolone (M groups, n=20), use Ulinastatin after SCI at half an- and 4- and 8- and 24- and 48- hour; administration of Ulinastatin and Methylprednisolone(M+U groups, n=20), use Ulinastatin and Methylprednisolone after SCI at half an- and 4- and 8- and 24- and 48- hour;Allen's weight drop model of acute spinal cord injury was prepared.

本课题利用Allen重物打击模型，选用SD大鼠80只，随机分为四组：脊髓损伤组(SCI组，n=20)；使用乌司他丁组(乌组，n=20)；使用甲基强的松龙组(甲组，n=20)；乌司他丁+甲基强的松龙联合用药组(乌+甲组，n=20)，所有给药组在脊髓损伤后(半小时、4小时、8小时、24小时、48小时)给药。

Objective To explore the changes of transcrainal magnetic stimulation motor evoked potentials and spinal cord pathology in rabbit spinal cord injury, and to explore their correlation. Methods A digital weight drop apparatus induced spinal cord lesion was performed to mimic human SCI.

目的 观察兔急性脊髓损伤后经颅磁刺激运动诱发电位(transcranial magnetic stimulation motor evoked pontentials， TMS-MEP)的变化规律，并与脊髓病理变化相比较，探讨其相关性。

B Class infectious diseases shall include viral hepatitis, bacillary and amebic dysentery, typhoid and para typhoid , AIDS, gonorrhea, syphilis, poliomyelitis, measles, pertussis, diphtheria, epidemic cerebrospinal meningitis, scarlet fever, epidemic hemorrhagic fever, rabies, leptospirosis, brucellosis, anthrax, epidemic and endemic typhus, epidemic encephalitis B, kala-azar, malaria, and dengue fever.

乙类传染病是指：病毒性肝炎、细菌性和阿米巴性痢疾、伤寒和副伤寒、艾滋病、淋病、梅毒、脊髓灰质炎、麻疹、百日咳、白喉、流行性脑脊髓膜炎、猩红热、流行性出血热、狂犬病、钩端螺旋体病、布鲁氏菌病、炭疽、流行性和地方性斑疹伤寒、流行性乙型脑炎、黑热病、疟疾、登革热。

Class infectious diseases shall include viral hepatitis, bacillary and amebic dysentery, typhoid and paratyphoid, AIDS, gonorrhea, syphilis, poliomyelitis, measles, pertussis, diphtheria, epidemic cerebrospinal meningitis, scarlet fever, epidemic hemorrhagic fever, rabies, leptospirosis, brucellosis, anthrax, epidemic and endemic typhus, epidemic encephalitis B, kala-azar, malaria, and dengue fever.

乙类传染病是指：病毒性肝炎、细菌性和阿米巴性痢疾、伤寒和副伤寒、艾滋病、淋病、梅毒、脊髓灰质炎、麻疹、百日咳、白喉、流行性脑脊髓膜炎、猩红热、流行性出血热、狂犬病、钩端螺旋体病、布鲁氏菌病、炭疽、流行性和地方性斑疹伤寒、流行性乙型脑炎、黑热病、疟疾、登革热。

Bacterial and amebic dysentery, tuberculosis, typhoid and paratyphoid, epidemic cerebrospinal meningitis, whooping cough, diphtheria, neonatal tetanus, scarlet fever, brucellosis, gonorrhea, syphilis, leptospirosis disease, schistosomiasis, malaria.

乙类传染病是指：传染性非典型肺炎、艾滋病、病毒性肝炎、脊髓灰质炎、人感染高致病性禽流感、麻疹、流行性出血热、狂犬病、流行性乙型脑炎、登革热、炭疽、细菌性和阿米巴性痢疾、肺结核、伤寒和副伤寒、流行性脑脊髓膜炎、百日咳、白喉、新生儿破伤风、猩红热、布鲁氏菌病、淋病、梅毒、钩端螺旋体病、血吸虫病、疟疾

Methods: To use ECM Gel as a tissue culture substrate, 3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.

采用ECM Gel作为组织培养的基质底物，将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养，观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion-induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions.

采用硅管套接大鼠切断的坐骨神经模型，局部给予胶质细胞源性神经营养因子，应用尼氏染色、酶组织化学染色方法，观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目，降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶及酸性磷酸酶变化的幅度。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。