脊髓

Lesion was made by current of 1mA, 210s in the dorsal funiculus at the 10th thoracic vertebral level. Behavioral and histological evaluation, HRP and fast blue retrograde labelling method were employed. The eletrolytic current caused bilateral corticospinal tract injury and incomplete paraplegia in hind limbs, and led to a substantial atrophy of corticospinal motoneurons , serving as a reproducible and practical model for research on SCI.

在大鼠T10胸椎节段脊髓后索插入损毁电极，通电1mA，210s，应用行为学评分、脊髓局部组织学检查、HRP和荧光逆行示踪方法发现，电解损伤造成了双侧皮质脊髓束纤维受损，大鼠双侧后肢肌力下降、肌张力升高、运动功能下降，同时大脑运动皮层处皮质脊髓束胞体发生明显逆行性萎缩，可作为一个稳定、可靠的SCI模型。

Fabrication of tissue section:after spinal cord injury ld,3d,5d,8d,14d,21d,we sacrificed rats in batch.4% formaldehydum polymerisatum were pured into the cardiac muscle and fixed for 24h.Then obtain the specimen of spinal cord from T_5 to T_(13).Establishing slices,HE dyeing,bcl-2 dectation,terminal doxynucleotidyl transferase-mediated dUTP nick end labeling.On the fourteenth day after spinal cord injury,we detect Gale grading score and tiltboard experiment.

组织切片的制备：脊髓损伤后1d，3d，5d，8d，14d，21d共6个时间段分批处死动物。4%多聚甲醛心肌灌注固定24h，切取每只大鼠T_5～T_(13)节段脊髓组织，常规制成切片，给于HE染色，Bcl-2检测，TUNEL原位末端标记以及伤后第14天检测Gale评分和斜板实验，检测大鼠脊髓损伤后细胞凋亡和脊髓神经功能恢复情况。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Poor appetite and bone marrow suppression were observed one week after the craniospinal radiation, and the radiation therapy was ceased. Spinal magnetic resonance imaging showed no spinal matastasis. White blood and platelet counts gradually increased. Subsequently, brain radiation was initiated. The patient currently can walk with good endurance and without device.

中断脑部脊髓放射治疗期间脊髓之核磁共振检查结果无脊髓之转移，白血球及血小板数目已有回升，所以重新开始脑部放射治疗而不再照射脊髓的部分，减少骨髓抑制的并发症；个案食欲不振的现象改善，且口语表达、步行及耐力亦进步中，并於脑部放射治疗结束后出院，改为门诊追踪。

Lipomyelomeningocele and diastematomyelia require more extensive surgical procedure.

脂肪脊髓脊膜膨出和脊髓纵裂则要求更加深入的手术治疗，脂肪脊髓脊膜膨出手术就是要尽可能的切除脂肪瘤，解除其对硬脊膜和脊髓的拴系。

Lipomyelomeningocele and diastematomyelia require more extensive surgical procedure. The goal of surgery in the former is to resect as much of the fatty mass as possible and free up the attachment of the fatty tissue, which has tethered the dura and the spinal cord.

脂肪脊髓脊膜膨出和脊髓纵裂则要求更加深入的手术治疗，脂肪脊髓脊膜膨出手术就是要尽可能的切除脂肪瘤照明和放大设施的改善大大增加了手术的准确性同时减少了手术的损伤。，解除其对硬脊膜和脊髓的拴系。

Conclusion］The spinal cord is damaged severely in double-tube diastematomyelia and gradually aggravated.Operation can get symptomatic relief in many patients and terminate spinal cord lesion. So operation should be performed as soon as the diagnosis is made. The spinal cord is damaged slightly in single-tube diastematomyelia and surgery is not indicated.

结论］双管型脊髓纵裂脊髓损害重，且呈进行性加重，精细的手术治疗可以使大多数患者症状缓解，终止神经进行性损伤，确诊后应尽早手术；单管型脊髓纵裂脊髓损害轻，不需手术探查。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

Baekgroud: Spinal cord ischemia reperfusion injury is an serious secondary spinal cord injury. Trauma, arteriosclerosis, vasal abnormality, inflammation, vasal infarct, decompression after the diseases that can bring spinal cord compression, lessening the blood stream of the spinal cord temporarily ,because of clamping the aorta during the operation of aorta, all of which can engender different extent spinal cord ischemia.

背景：脊髓缺血再灌注损伤是严重的脊髓继发性损伤，创伤、动脉硬化、血管畸形、炎症、血管梗塞、各种产生脊髓压迫的疾病减压后以及在主动脉手术中，钳夹主动脉导致脊髓血液供应暂时性减少都可造成不同程度的脊髓缺血再灌注损伤。

Trauma, arteriosclerosis, vasal abnormality, inflammation, vasal infarct,decompression after the diseases that can bring spinal cord compression, lessening the blood stream of the spinal cord temporarily ,because of clamping the aorta during the operation of aorta, all of which can engender different extent spinal cord ischemia.

背景：脊髓缺血再灌注损伤是严重的脊髓继发性损伤，创伤、动脉硬化、血管畸形、炎症、血管梗塞、各种产生脊髓压迫的疾病减压后以及在主动脉手术中，钳夹主动脉导致脊髓血液供应暂时性减少都可造成不同程度的脊髓缺血再灌注损伤。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。