脂酶的

Objective: The purpose of our studies are gaining a better insight into the effect of genistein on mesangial cell"s phenotype change and ECM expression plus degradation under DN circumstances.We add genistein with different concentrations in high glucose cultured rat mesangial cells;feed DM rats with the single concentration genistein for different days;to explore the expression of SMemb,the mark of mesangial cell"s hypertrophy phenotype change;to examine the synthesis of Fn as well as the change of the balance between MMP-2 and TIMP-l;to comprehend its effect on renal pathological changes of DM rats and some related factors;trying toexplore the possible mechanisms.Through these studies,we will have some information about the experimental value of genistein in DN field.

目的：为了研究金雀异黄素对DN环境下大鼠MC肥大表型转换标志物SMemb和ECM分泌及降解的影响，我们拟在细胞水平以不同浓度的金雀异黄素作用于高糖培养下大鼠MC，在动物水平以同一浓度的金雀异黄素作用于DM大鼠不同时间，观察细胞表型转换标志物SMemb、ECM主要成分Fn、ECM降解酶系统基质金属蛋白酶—2(MMP-2)／基质金属蛋白酶抑制剂—1(TIMP-1)表达的变化，同时观察金雀异黄素对DM大鼠肾脏病理及影响肾脏病进展的糖脂代谢紊乱的影响，并进一步探讨金雀异黄素发挥作用的可能机制；为明确金雀异黄素在DN治疗中的地位及意义提供实验依据。

Treatments with 20μmolL^(-1) CoCl2 and 20μmolL^(-1) AVG did not influence the contents of putrescine, spemidine, spermine, chlorophyll, and the production rate of reactive oxygen species. However, after 12h heat stress of 45℃, treatments significantly promoted the above values.

但经45℃高温胁迫12h后，20μmolL^(-1) CoCl2与20μmolL^(-1) AVG溶液处理显著提高了上述3种多胺的含量，减缓了抗氧化酶活性的下降，对活性氧水平及脂质过氧化水平的增加也起到了有效的抑制作用，这些改变可能对叶绿素降解的减少及膜稳定指数下降的减轻发挥着重要的作用。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

In this paper, the perturbations of CTX-IX, which is a kind of cardiotoxin isolated from Naja naja atra, on artificial liposome, reconstituted system of dellpidated ATPase and phospholipld and mouse lymphacyte were investigated in order to understand the membrane toxlcology of CTX-IX in view of molecular dynamics of membrane, give clues for searching new toxic polypeptides that could kill the harmful cells and establish a example for the study of lipld-protein interaction and corresponding significance in membrane function.

本文研究了广东中华眼镜蛇毒中分离得到的心脏毒素CTX-IX和人工磷脂水化聚集体、ATP酶-磷脂重组体系和小鼠脾细胞之间的相互作用，试图从膜分子动力学角度理解心脏毒素的膜毒理，为杀伤细菌等有害细胞的毒性肽的寻找提供线索，在理论上亦为脂蛋白相互作用与膜结构功能的关系研究提供一个范例。

The results showed as:(1)Gegen decreases fasting insulin and insulin resistance index and increases insulin sensitive index of rats of insulin resistance induced by dexamethasone significantly;(2)Gegen can improve glucose tolerance of rats of insulin resistance but not to alloxan indued diabetes mice;(3)Gegen increases glucose intake of 3T3-L1 adipocytes and improves the insulin resistance of 3T3-L1 adipocytes induced by dexamethasone significantly;(4)Gegen can activate peroxisome proliferator- activated receptor gamma, make it bind to PPAR response elements;(5)Gegen can up-regulate mRNA expression of PPAR v ,aP2 and adiponectin.

结果表明：①葛根显著降低地塞米松诱导的胰岛素抵抗大鼠血清胰岛素水平和胰岛素抵抗指数，升高胰岛素敏感指数，改善大鼠的胰岛素抵抗；②葛根显著改善胰岛素抵抗大鼠的糖耐量，但对四氧嘧啶糖尿病小鼠的糖耐量没有明显的作用；③葛根显著增加胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取，改善地塞米松诱导的3T3-L1脂肪细胞的胰岛素抵抗；④葛根能促进HepG2肝癌细胞过氧化物酶体增殖物激活受体γ与靶基因的特异DNA序列结合，对PPARγ及其下游基因的转录具有激活作用；⑤葛根能够促进PPARγ及其下游基因脂肪酸结合蛋白(aP2)、脂联素mRNA的表达。

These parameters did not differ from each other in figleaf gourd"s two treatments. The root temperature of 14℃didn"t induce significant changes in the total respiration rate in both species but caused an increase of the alternative respiration pathway and a decrease of the cytochrome respiration pathway, and more dead cells in root tips in cold-sensitive cucumber roots.

低温条件下，黄瓜的生长明显差于黑籽南瓜，同时根系表现出更高的超氧化物阴离子O_2~(·-产生速率、过氧化氢(H_2O_2)与膜脂过氧化产物丙二醛含量以及抗氧化酶活性。14℃根温并未引起两种作物根系的总呼吸速率发生变化，但却引起黄瓜根系的交替途径呼吸速率与细胞色素途径呼吸速率的显著变化，表现为低温下Valt的上调而Vcyt的下降。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。