脂酶的

Observation was made on the content of malondialdehyde. Results Compared with MC group, the increases of MDA content in the membrane induced by Xan?XO system, H2O2 or UV light in all of the three Punica granatum L.group were inhibited dose?dependently.

结果 同MC组相比，三组石榴皮提取物对黄嘌呤－黄嘌呤氧化酶系统、H2O2及UV照射3种方法引起的细胞膜脂质过氧化产物丙二醛的生成增加均有抑制作用，且有一定的剂量依赖关系。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

However,compared to Cd~(2+) stress alone, treatment of SNP+Cd~(2+) caused more increases in SOD,CAT and POD activities, a decrease of H_2O_2 level and MDA content in leaf and root ofmung bean seedlings. These results indicated that exogenous NO induced increases inSOD, CAT and POD activities, which scavenged H_2O_2 and resultantly inhibitedmembrane lipid peroxidation in leaf and root of mung bean seedlings under Cd~(2+) stress.

说明NO通过诱导膜保护酶活性的提高，降低了活性氧水平，从而使Cd~(2+)胁迫诱导的膜脂过氧化程度降低，这可能是外源NO明显缓解Cd~(2+)胁迫抑制绿豆幼苗生长发育的原因之一。

To investigate the effects of lipoxygenase (LOX-1) on the cormels formation and enlargement in Gladiolus hybridus, with 'Rose Supreme' and 'Advanced Red' as materials, LOX-1 activity, endogenous methyl jasmonate, sucrose, starch and cellulose contents were examined during the cormels growth.

为探讨脂氧合酶(LOX-1)对唐菖蒲籽球形成和膨大的影响，以'Rose Supreme'和'Advanced Red'两个品种为试材，研究了籽球的生长发育与LOX-1活性，内源茉梨酸甲酯、蔗糖、淀粉和纤维素含量变化之间的关系。

The treatment comparison by using Chinese TANG Kidney capsule and Western medicine TANG SHI PIN showed: the kidney capsule can lower the values of the blood sugar, blood fat, series of micro-protein un urine and carbon monoxide in plasma, thus the function of kidney can be improved, and prolong the process of deadness and hardening of the affected kidney small cell fibre.

利用该模型选择中药糖肾胶囊与西药糖适平对照治疗研究，结果显示：糖肾胶囊能降低糖尿病肾病大鼠的血糖、血脂、尿素氮、肌酐、尿系列微量蛋白及血一氧化氮等指标，从而减轻糖尿病肾病大鼠肾小球的高灌注高滤过，降低糖化血红蛋白含量，减轻非酶糖基化，改善肾功能，降低血脂、内皮素、调节血栓素、前列腺素水平，有抗脂质过氧化损伤的作用。

Uricase inhibitor was given subcutaneously by lanolin mixed with olefin in acute animal model with Hyperuricemia on rat, which has the characters of high uric acid and maintaining a long time.

以羊毛脂石蜡油（质量比3：2）为溶剂配制尿酸酶抑制剂，采用皮下注射的方法造模，所复制的大鼠代谢性高尿酸血症模型具有血尿酸值高、维持时间长的特点。

Aiming at the unbalance of the diabetes patients which owing to metabolism turbulence of blood glucose, protein, fattiness, water and electrolyte, and adopting scientific herbal prescription through enzymolysis, extraction and concentration separates the crystal of "active blood-regulating plant insulin ". It can fully restore and reinforce the function of insulin, decompose or melt the fattiness materials. For the people whose blood glucose is very high or can't reduce stiffly, it can quickly eliminate the excessively eat, drink and urine complications etc. For the patients with slight symptom, low-dosage treatment can stabilize the blood glucose and avoid the complications.

针对糖尿病人血糖、蛋白质、脂肪、水及电解质代谢紊乱导致的人体平衡失调，科学组方中草药，经过酶解、萃取、浓缩，分离出被中西方医学界称为&植物活胰调血素&的晶体，具有全面修复和增强胰岛功能，分解消溶脂类物质，针对血糖过高或顽固不降的人群使用，能够快速消除多食、多饮、多尿、四肢无力、心慌胸闷，小便发白，视力下降等糖尿病并发症。

After seedling trees of Yunhe Snow Pear were polluted by Pb(superscript 2+), Cd(superscript 2+), synthesis of chlorophyll was restrained, toxic effect of Pb(superscript 2+), Cd(superscript 2+) on seedling trees was intensified, membrane lipid peroxidation damage was increased. And the protective enzymes system start-up of polluted seedling trees enhanced antioxidative enzymes activities of seedling trees.

结论］Pb(上标 2+)、Cd(上标 2+)污染云和雪梨苗，抑制叶绿素的合成，增强超养阴离子自由基O2(上标-对植株的毒害性，使膜脂过氧化损伤程度高，受重金属污染植株启动保护酶系统，以增强植株的抗氧化性。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。