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Here, we investigated the roles of the PM NADPH oxidase in nickel-induced enhancement in production of ROS and the activity of antioxidant enzymes such as SOD, CAT and GR in roots of wheat Triticum durum D.

而且,镍导致ROS增加以及抑制剂对这些参数抑制的规律与镍和抑制剂对质膜NADPH氧化酶活性影响的规律类似,这些结果证明,镍诱导产生的ROS导致了膜脂过氧化,而这些ROS主要来自质膜NADPH氧化酶。

The present study analysis whether CRP could directly induce inflammation in endothelial cells, analysis what kind of effects CRP provide on cellular signal transduction in endothelial cell by gene array, and isolate LDL by one step of density gradient centrifugation, generate E-LDL by degrading LDL with multiple enzymes, direct fluorescent labeling CRP and LDL, investigate whether CRP play as intermediator in atherosclerosis.

本课题目的在于探讨CRP在脂质参与的动脉粥样硬化形成中是否起到介质作用,主要通过检测直接CRP能否诱导内皮细胞炎症表现,基因芯片分析CRP对内皮系统的细胞信号转导通路的影响,探讨CRP参与调控内皮系统炎症过程的主要机制,并且通过一步法密度梯度离心分离LDL,复合酶处理生成E-LDL,直接荧光标记CRP和LDL,分析CRP介导脂质紊乱尤其是LDL代谢产物参与动脉粥样硬化早期形成的作用。

Vitamin E, or tocopherol, is a lipid-soluble antioxidant and essential for human health. 2-methyl-6-phytyl-1, 4-benzo- quinol methyltransferase, one of the key enzymes in vitamin biosynthetic pathway in plants, converts 2-methyl- 6-phytyl-1,4-benzoquinol to 2,3-dimethyl-6-phytyl-1,4-benzoquinol.

维生素E是一类人体必需的脂溶性抗氧化剂,具有重要的生理功能。2-甲基-6-叶绿基-1,4-苯醌甲基转移酶是天然维生素E合成途径中的关键酶之一,催化MPBQ甲基化,生成DMPBQ。

Results The highest drug use frequency was biguanide. There was 50.9% in the oral antidiabetic drug combination. Leading the first 3 places on the list of drug combination were sulfonylureas in combination with biguanide, sulfonylureas in combination with α-Glycosidace inhibitor, biguanide in combination with α-Glycosidace inhibitor, while there was 16.1% in 3 kinds combination. When oral antidiabetic drugs were combined with other drugs, combination with antihypertensives drugs led the fist, while with antihypertensives drugs led the second.

结果 二甲双胍使用频率最高,口服降糖药联合用药的比例高达50.9%,居前3位的依次是磺脲类+双胍类、磺脲类+α-葡萄糖苷酶抑制剂和双胍类+α-葡萄糖苷酶抑制剂,而3种以上口服降糖药联合用药的比例也达16.1%;口服降糖药与其他药物联合使用情况看,配合使用抗高血压药的频率最高,其次是降脂药。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体;用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠,用血凝抑制实验和酶联免疫吸附试验对免后2~13周的小鼠血清的抗体水平进行检测,HI检测其特异性抗体,ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

Iba 2002. Both the Arabidopsis fad5 mutant, which lacks an active chloroplast x-9 fatty acid desaturase and accumulates high levels of palmitic acid (16:0), and the fad6 mutant, which lacks an active x-6 fatty acid desaturase and accumulates high levels of palmitoleic acid (16:1) and oleic acid (18:1), have reduced levels of polyunsaturated fatty acids in the chloroplast galactolipids.

两个拟南芥突变体的叶绿体半乳糖脂含有较少的多不饱和脂肪酸:一种是拟南芥fad5突变体,它缺乏有活性的叶绿体的X-9脂肪酸去饱和酶,因而积累了高浓度的棕榈酸( 16:0 );另一种是拟南芥fad6突变体,它缺乏一个有活性的 X - 6脂肪酸脱氢酶因而积累了高浓度的软脂酸( 16:1 )和油酸( 18:1 )。

It indicate that 200μmol/m2s and 80μmol/m2s low light cause reactive oxygen species quick producing and SOD and CAT acticity enhance, but the latter's increasing cann't offset reactive oxygen species'quick producing, which lead to membrance peroxidize injury on peach tree. 30μmol/m2s low light's injury on peach tree mainly because reactive oxygen species'accumulation.

表明200μmol/m2s和80μmol/m2s的弱光下桃树抗氧化酶活性的诱导提高不足以抵消弱光下自由基的快速生成,从而导致对桃树细胞膜脂的伤害;30μmol/m2s的弱光下桃树活性氧的快速生成加剧膜脂过氧化程度,是造成弱光对桃树伤害的重要原因之一。

The concentrations or activities of selenium,copper,zinc and MDA or GSH-Px,SOD ,CuZn-SOD and CAT in infested and healthy Rangifer tarandus sera were detected respectively in order to explore relationship between C.trompe infestation and metabolism of oxygen free radical. Results were as follows.

为探讨驯鹿狂蝇蛆病与机体氧自由基代谢的关系,分别对感染驯鹿和健康驯鹿血清中的微量元素硒、锌、铜、谷胱甘肽过氧化物酶、超氧化物歧化酶、铜锌超氧化物歧化酶、过氧化氢酶和脂质过氧化终末产物丙二醛的含量进行了测定。

The reason why only when wool is pretreated with hydrogen peroxide can protease SZ confer wool excellent antifelting property is that the pretreatment can remove some lipid on epicuticle, thus the protease SZ has the possibility to digest the protein of epicuticle for no lipid's obstruction.

SZ酶之所以需在双氧水氧化预处理的条件下才能使羊毛织物获得优良的防毡缩性能,是因为双氧水预处理在打开羊毛蛋白部分交联的同时,也可去除羊毛鳞片表面部分类脂,为SZ酶进攻羊毛鳞片表层蛋白提供了可能。

Coli might be distributed in all the gene. The intact Hu-P68 cDNA ORF including 10bp upstreams of ATG were obtained by PCR deletion and reconstruction. The resulted fragment were cloned into transfer vector pBacPAK〓 at Bam HI site and lipofectioned into 〓 cells with Bsu 36I digested genomic DNA of insect baculovirus BacPAK 〓.

利用PCR技术删除人P68 cDNA翻译起始位点上游10bp以外的5'UTR,并引入BamHI位点,经酶切和测序验证后,将此改造的完整P68 cDNA基因编码区装入转移载体pBacPAK〓,用脂转染法将此重组质粒与酶切的昆虫杆状病毒BacPAK〓基因组DNA片段导入昆虫细胞〓中,经同源重组获得了再生病毒。

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