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One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,"PMN-EC"相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

This paper deals with the changes in ATPase and PPase activities, lipid compositon of tonoplast vesicles from two wheat cultivars, Altas 66(Al-tolerant) and Scout 66(Al-sensitive) under Al stress.

刘友良 ,沈振国,何龙飞研究了铝胁迫下耐铝性不同的两个小麦品种根细胞液泡膜ATP酶、焦磷酸酶活性和膜脂的变化。

WT, antisense-transgenic lines T2-8 and sense- transgenic lines T2-7 of tomato were used to measure the fatty acid composition of thylakoid membrane, photosynthetic and chlorophyll fluorescence parameters, chloroplastic reactive oxygen species and activities of antioxidant enzymes under salt stress.

以野生型,转反义番茄叶绿体ω-3脂肪酸去饱和酶(LeFAD7)基因株系T2-8和转正义LeFAD7基因株系T2-7为试材,测定了类囊体膜脂脂肪酸组成,盐胁迫后的光合参数,叶绿素荧光参数和叶绿体活性氧清除酶活性。

A method for determination of Secoisolariciresonol diglucoside, secoisolariciresinol, enterolactone and enterodiol in serum by LC/MS/MS was established. In this method,β-Glucuronidase was used to hydrolyze the serum samples, the electrospray ionization sourse and negative ion MRM scan were employed.

本实验用β葡萄糖醛酸苷酶酶解血清样品,采用电喷雾离子源,负离子MRM扫描,建立了血清中司可异罗叶松甘油二酯、司可异罗叶松酯素、肠内二醇、肠内脂的IC/MS/MS分析方法。

The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1,2-FT活性的改变,采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂,特别是Ⅱ型寡糖的变化。

Objective : To detect the serum level of triglyceride、total cholesterol、low-density-lipoprotein-cholesterol、plasma viscosity,fibrinogen 、the level of plasma tissue plasminogen activator,plasminogen activator inhibitor-1(PAI-1) and calculate the score of nervous impairment of acute ischemia apoplexy patients ; so as to compre- hend changes of lipid metabolism、hemorheology and fibrinolysis function of AIA patients and it relation of the condition degree, and to investigate relationship between the above-mentioned microcosmic indexes and phlegm blood stasis syndrome.

目的:通过观察急性缺血性中风患者血清甘油三酯、胆固醇、低密度脂蛋白、血浆粘度、纤维蛋白原、血浆组织纤溶酶原激活物及其抑制剂-1(PAI-1)和评定神经功能缺损程度积分以了解急性缺血性中风患者脂质代谢、血液流变性及纤溶系统功能的变化及其与病情程度的关系,并探讨上述微观指标与痰瘀证的关系。

The proportion of GGT complexed with low density lipoproteins plus very-low density lipoproteins in total GGT in serum is determinated with ratio colorimetery,sodium phosphotungstate and MgCl2 as precipitation.26 patients with liver cancer,18 with liver cirrhosis,22 with chronic hepatitis,14 with the other malignant tumors,16 with non-hepatobiliary disease of high GGT and 50 healthy subjects have been evaluated.

本文以磷钨酸-氯化镁作沉淀剂,速率法测定血清低密度脂蛋白,极低密度脂蛋白结合的r-谷氨酰转移酶(LDL-VLDL-GGT)占总GGT的百分比。对临床26例肝癌、18例肝硬化、22例慢性肝炎、14例其他恶性肿瘤、16例GGT升高的非肝脏疾病及50例健康人进行了检测,结果表明:肝癌组明显高于其他各组,差异显著(P<0.01)。肝脏疾病升高幅度:肝癌>肝硬化>慢性肝炎,且每两者间有显著差异(P<0.01)。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,&PMN-EC&相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

In order to improve the foaming properties of egg albumen powder, the lipase was applied to hydrolysis lipids of yolk.

为减少混入的蛋黄中脂类物质对蛋清粉起泡性的影响,该试验采用脂肪酶水解脂类来提高蛋清粉起泡性能。

The dosage of aspirin,overdose of epoxidase 2,effect of arachidonic acid lypoxygenase metabolic pathway,multiplicity of glucoprotein Ⅱb/Ⅲa acceptor hypotype and activating route of other thromboplastid can cause aspirin resistance,this article reviews the mechanism of aspirin resistance.

阿司匹林服用剂量、环氧化酶2过量表达、花生四烯酸脂氧化酶代谢途径的作用、糖蛋白Ⅱb/Ⅲa 受体亚型多样性及其他血小板活化途径的可能作用均可能引起阿司匹林抵抗,本文就阿司匹林抵抗机制进行综述。

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