脂酶

The content of lipid peroxide was determined with fluorescence method, and the activity of superoxide dismutaseand glutathione peroxidase was determined with self-oxidation method of pyrognllol and Hafenam method, respectively.

脂质过氧化物含量按荧光法测定，超氧化物歧化酶活性应用邻苯三酚自氧化法测定，谷胱甘肽过氧化物酶活性按Hafenam方法测定。

These parameters did not differ from each other in figleaf gourd"s two treatments. The root temperature of 14℃didn"t induce significant changes in the total respiration rate in both species but caused an increase of the alternative respiration pathway and a decrease of the cytochrome respiration pathway, and more dead cells in root tips in cold-sensitive cucumber roots.

低温条件下，黄瓜的生长明显差于黑籽南瓜，同时根系表现出更高的超氧化物阴离子O_2~(·-产生速率、过氧化氢(H_2O_2)与膜脂过氧化产物丙二醛含量以及抗氧化酶活性。14℃根温并未引起两种作物根系的总呼吸速率发生变化，但却引起黄瓜根系的交替途径呼吸速率与细胞色素途径呼吸速率的显著变化，表现为低温下Valt的上调而Vcyt的下降。

MethodsAfter construction of a fulllength human βNGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0NGF.

方法构建βNGF的真核表达载体pcDNA3.0NGF，经酶切鉴定后，采用脂质体lipofectamine转染人胆管癌细胞株QBC939。

The protein content, activity of SOD and GSHPX were measured in liver homogenates.

脂质过氧化物歧化酶及谷胱甘肽过氧化物酶活性，并观察肝组织学变化。

Experimental Center of National Level One, Hubei Traditional Chinese Medica l College, Wuhan 430061）Abstract To investigate the protective effects of Jiaweisinisa n on acute liver injury induced by intraperitoneal injection of D-ga lac-tosamise, serum superoxide dismutase, malondialdehyde , glutathioe contents were measured. The pathological changes of liver wer e observed simultaneously. The results showed that JWSNS could decrease serum MD A content, but increase serum SOD and GSH activities in acute liver injury induc ed by D-GalN. It was also found that JWSNS could alleviate the degeneration and necrosis of the hypatocytes.

为研讨加味四逆散防治肝损伤的作用机理，通过动物试验，先用加味四逆散预防给药，再采用D-氨基半乳糖造成大鼠急性肝损伤模型，然后检测大鼠血清超氧化物岐化酶、丙二醛、还原型谷胱甘肽水平，同步观察该方对急性肝损伤大鼠体内脂质过氧化反应及肝脏病理改变的影响，结果表明该方可明显升高大鼠血清SOD、GSH水平，降低MDA水平，肝脏病理学检查亦表明该方使肝细胞变性坏死程度减轻。

The Arabidopsis fad2 mutant, deficient in an endoplasmic reticulum localized x-6 desaturase, had significantly decreased polyunsaturates in the extrachloroplast membrane lipids.

拟南芥fad2突变体则缺乏一个内质网定位的X-6去饱和酶，它的多不饱和脂肪酸在叶绿体外膜脂中含量显著减少。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

Objective To explore the activities and the level of antioxidant system in Parkinson disease and the effect of L-dopa and Vit E on it,and find the indications of oxidative stress in PD.

目的 探讨帕金森病的抗氧化酶活性和过氧化脂质代谢水平的变化和多巴药物及Vit E对其的影响，找出反映氧化异常的客观生化指标。

Objective To explore the activities and the level of antioxidant system in Parkinson disease and the effect of L-dopa and Vit E on it,and find the indications of oxidative stress in PD.

摘 要：目的探讨帕金森病的抗氧化酶活性和过氧化脂质代谢水平的变化和多巴药物及Vit E对其的影响，找出反映氧化异常的客观生化指标。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。