脂酶

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

The test measured the inhibition of binding between a labeled,monoclonal antibody 3-47-0 and Salmonella pullorum lipopolysaccharide.

该方法基于待检血清样品抑制酶标单抗与脂多糖的结合，以检测鸡白痢和鸡伤寒特异性抗体。

The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

该载体经PacI酶切，脂质体法转染人胚肾293A细胞，获得重组腺病毒载体pAD-E7.pAD-E7转染人HaCaT细胞，激光共聚焦显微镜分析E7蛋白表达情况。

Comparative study on the change of lipid peroxidation product and activities of regulatable enzymes by irradiation of N + ion beam and 60Co γ-rays .

顾月华，罗江虹，罗建平，等。N+离子束和60Co γ射线辐照引起脂质过氧化产物及调节酶活性变化的比较研究，核技术，1999，22(8)：44-48。

The results showed that mitochondria lipid peroxidation level significantly elevated, SOD activity and GSH content remarkedly reduced after exercise immediately.

发现运动后即刻心肌线粒体脂质过氧化水平显著升高，超氧化物岐化酶活性和还原型谷胱甘肽含量显著下降。

However,compared to Cd~(2+) stress alone, treatment of SNP+Cd~(2+) caused more increases in SOD,CAT and POD activities, a decrease of H_2O_2 level and MDA content in leaf and root ofmung bean seedlings. These results indicated that exogenous NO induced increases inSOD, CAT and POD activities, which scavenged H_2O_2 and resultantly inhibitedmembrane lipid peroxidation in leaf and root of mung bean seedlings under Cd~(2+) stress.

说明NO通过诱导膜保护酶活性的提高，降低了活性氧水平，从而使Cd~(2+)胁迫诱导的膜脂过氧化程度降低，这可能是外源NO明显缓解Cd~(2+)胁迫抑制绿豆幼苗生长发育的原因之一。

BACKGROUND ＆ AIM: To investigate the changes of semicarbazide- sensitive amine oxidase activity in lipopolysaccharide-induced pulmonary inflammation.

背景与目的：探讨SSAO酶在脂多糖诱导急性肺损伤过程中的活性变化。

Identification of cripto-1 as a novel serologic marker for breast and colon cancer［J］.

脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性［J］。

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的：观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法：选用健康更年期雌性sd大鼠，随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水，实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮，连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力，用跳台实验方法测定大鼠学习记忆能力。结果：与空白对照组比较，中、高剂量组大鼠血清mda含量显著降低，低、中、高剂量组血清sod活力明显升高，中、高剂量组血清gsh-px活力明显升高。结论：大豆异黄酮具有明显的抗氧化作用，并有效提高更年期雌性sd大鼠的学习记忆能力。

Keywords : Pomegranate leaf tannide;HMG-CoA redutase;hyperlipidemic model

中文关键词：石榴叶鞣质；HMG-CoA还原酶；高脂模型

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。