脂酶

Methods The cognitive impairment rat model was induced by hypodermic D-galactose injection and half fat feeder (6 weeks), the Morris water maze was used to screen the model rats, which were divided randomly into 4 groups (12 rats in each group): the model group, the hydrochloricdonepezil group (0.4 mg/kg, weight, sic passim), the low-dose Huannao Yicong Fang group (7 g crude drug/kg), the high-dose Huannao Yicong Fang group (14 g crude drug/kg), they were treated respectively by intragastric administration. Besides, a control group of 12 rats was set up, they were given tales doses of distilled water. After 4 weeks, the behavior of the rats was detected, the contents of plasma P-selectin and PAI-1 were measured by ELISA, also the hemorrheology was measured.

采用皮下注射D-半乳糖及喂饲半高脂饲料（6周）的方法造成大鼠认知功能障碍，Morris水迷宫行为学测试确定模型成功的动物，随机分成四组（每组12只：模型对照组给予等量水，盐酸多奈哌齐组（0.4mg/kg，体质量，下同），还脑益聪方低剂量组（7g生药／kg），还脑益聪方高剂量组（14g生药／kg），同时灌胃给药，并设立正常对照组，给予等量水。4周后，对大鼠行为学检测，应用酶联免疫法检测大鼠血浆P-选择素及PAI-1含量和血液流变学各项指标。

Methods SH-SY5Y cells, a human neuroblastoma cell line, were incubated with different concentrations of fluoride for 48 hr and somle of them were treated with vitamin E precedently. The functional situation of cells was measured by MTT method; lipid peroxidation was detected by High-Performance Liquid Chromatography; phospholipid was separated by a Silica SepPak cartridge and neutral lipids by HPLC.

体外培养SH-SY5Y人脑神经母细胞瘤细胞，在培养液中加入不同浓度的氟化物或加入抗氧化剂，培养48h后用测定细胞MTT的方法来了解细胞的损伤程度，用高效液相色谱法分离和测定培养液中脂质过氧化物水平，用过柱和比色法测定细胞生物膜磷脂含量，用高效液相色谱法测定细胞生物膜辅酶Q和胆固醇含量。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Effects on protective enzyme activities of tomato seedlings under different concentration of abscisic acid, putrescine and epibrassinolide were investigated.

笔者研究了亚适温下不同浓度的脱落酸、腐胺、油菜素内脂对冷敏品种番茄"中蔬6号"幼苗保护酶活性的影响。

Treatments with 20μmolL^(-1) CoCl2 and 20μmolL^(-1) AVG did not influence the contents of putrescine, spemidine, spermine, chlorophyll, and the production rate of reactive oxygen species. However, after 12h heat stress of 45℃, treatments significantly promoted the above values.

但经45℃高温胁迫12h后，20μmolL^(-1) CoCl2与20μmolL^(-1) AVG溶液处理显著提高了上述3种多胺的含量，减缓了抗氧化酶活性的下降，对活性氧水平及脂质过氧化水平的增加也起到了有效的抑制作用，这些改变可能对叶绿素降解的减少及膜稳定指数下降的减轻发挥着重要的作用。

To understand how to use differential centrifugation to separate organelle and biological molecular;microspectrophotometry;immunofluorescence technifue and immune electron microscopy;in situ hybridization; aser scanning confocal microscopy;the method to show the nucleic acid、protein、enzyme、glucide and lipid; negative staining; freeze etching and quick freeze deepetching; flow cytometry.

了解用超速离心技术分离细胞器与生物大分子；显微分光光度测定技术；免疫荧光技术与免疫电镜技术；原位杂交技术；激光共焦点扫描显微镜技术；细胞内核酸、蛋白质、酶、糖类与脂质等的显示方法；负染色技术冷冻断裂和冷冻蚀刻电镜技术；流式细胞仪。

In this study, the effect of cholesterol, polyethylene glycol, pH value and temperature of rehydration no the encapsulation qualities were studied.

研究了胆固醇、聚乙二醇、水化温度和pH对脂质体包封辅酶Q10效果的影响。

Product characteristic: The utensil is natural dissolve enzyme formula, delivers effective matter to the skin inner urge a fat to eliminate secreta with natural row, deep of excessive sebum tier.

产品特点：具天然溶酶方子，将有效物质送到肌肤内层，促使油脂粒与过剩皮脂的自然排，深层断根分泌物。

Objective The purpose of this study was to investigate the joint effect of vitamin C,vitamin E or selenium on cell proliferation,lipid peroxidation and anti-oxidase enzymes induced by cadmium in LLC-PK 1 cells.

研究氯化镉(CdCl2 )及其分别与维生素C、维生素E、硒联合作用对猪肾近曲小管上皮(LLC PK1)细胞增殖、脂质过氧化及抗氧化酶的影响。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。