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These genes were involved in pathways of lipids metabolism, ketone body production, enzymatic and transcriptional regulation, most of which were consistent with results of metabonomic investigation.

肥胖易感与肥胖抵抗大鼠转录组学比较发现两组动物肝脏中有近80种基因在转录水平表现出显著差异(≥1.5倍),差异表达的基因主要涉及到脂质代谢、酮体生成、酶调节和转录调节等代谢通路。

Adipo R2 and Krs genes were lowest expressed in EA group. The genes of TNF-αand Utr were lowest expressed in the groups of EN and ST, respectively. This result was coincidence with that of SSH.

对脂联素受体2(Adiponectin recptor 2,AdipoR2)、赖氨酸tRNA合成酶(lysyl-tRNA synthetase,Krs)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、Dystrophin相关蛋白(Utrophin,Utr)等4个基因进行定量PCR检测,在不同处理组小鼠肝脏中的表达情况有所不同。

This study can be prepared for further investigation in future. Adipose tissue was collected from different female(from 32-45) undergoing elective suction-assisted lipectomy procedures.

利用整形外科成熟的负压吸脂术获取脂肪组织,经过一定处理后以酶解法得到具有增殖分化能力的幼稚细胞,此类细胞被称为PLA细胞,而后在体外培养扩增。

The research indicated that the addition of biosurfactant rhamnolipid effectively improved the enzymatic hydrolysis of cellulose in organic solvent mediae.

研究表明生物表面活性剂鼠李糖脂可有效促进有机相体系中纤维素的酶水解。

Efficient usage of the main storage material in megagametophyte before it falls off from seedling is important for seed germination of Pinus edulis Engelm.. We examined and compared lipid usage in both megagametophyte and cotyledon with electromicroscopic technique, the length, dry weight, starch content and FBPase activities of each part of the seedling were measured and compared.

采用电镜技术观察了黑暗中萌发中食松子叶和提供营养的雌配子体中脂肪体和与脂肪利用有关的乙醛酸循环体的变化,测量比较了子叶、下胚轴、根的长度、干重、淀粉含量,以及果糖1,6二磷酸酶的活性变化,对贮藏物质脂类、淀粉的利用及与生长的关系进行了分析。

Lipid bilayer membrane containing monomeric bovine cytochrome c oxidase was successfully immobilized in gold quartz crystal microbalance electrodes.

含有单体牛细胞色素c氧化酶的脂质双层膜,被成功地固定在金石英晶体微平衡电极上。

Objective To study the effects of formaldehyde on the lipid peroxidation and antioxidase in mouse brain tissues to explore the neurotoxic action of formaldehyde and its mechanism.

目的:通过观察甲醛对小鼠脑组织脂质过氧化及抗氧化酶的影响,探讨甲醛对小鼠的神经毒性及作用机理。

The results were as follows: under light or medium water stress, the changes of RWC, Pro, RCC, MDA and SOD were similar, membrane penetration and membrane lipid peroxidation aggravated, osmotic modulation ability and enzyme protection system strengthened; physiological modulation ability declined under severe water stress. Almonds subjected to drought in the condition of below 3.77% soil water content. Altogether Fritz and Nonpareil both had stronger resistant drought adaptation.

结果表明,在受到轻度和中度胁迫过程中,扁桃幼苗叶片的Pro、RCC、MDA以及SOD都出现相似变化趋势,细胞膜透性和膜脂过氧化程度逐渐加剧,渗透调节能力和酶保护系统增强;在重度胁迫时,其生理调节能力下降,当达到土壤含水量3.77%以下的重度胁迫时,就会受到旱害;总体而言,两个供试品种扶兹和那普瑞尔都具有较强的抗旱适应性。

Studies on serum level of lipid peroxidation and activity of anti oxidase in Quarrymem [J].

采石工人血清脂质过氧化水平和抗氧化酶活性的研究[J]。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。