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The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1,2-FT活性的改变,采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂,特别是Ⅱ型寡糖的变化。

Objective : To detect the serum level of triglyceride、total cholesterol、low-density-lipoprotein-cholesterol、plasma viscosity,fibrinogen 、the level of plasma tissue plasminogen activator,plasminogen activator inhibitor-1(PAI-1) and calculate the score of nervous impairment of acute ischemia apoplexy patients ; so as to compre- hend changes of lipid metabolism、hemorheology and fibrinolysis function of AIA patients and it relation of the condition degree, and to investigate relationship between the above-mentioned microcosmic indexes and phlegm blood stasis syndrome.

目的:通过观察急性缺血性中风患者血清甘油三酯、胆固醇、低密度脂蛋白、血浆粘度、纤维蛋白原、血浆组织纤溶酶原激活物及其抑制剂-1(PAI-1)和评定神经功能缺损程度积分以了解急性缺血性中风患者脂质代谢、血液流变性及纤溶系统功能的变化及其与病情程度的关系,并探讨上述微观指标与痰瘀证的关系。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化,CD44免疫组化法分析ADSCs表面标志;用含成骨、成脂诱导培养基培养4周后,行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

The results of this research implicated that betaine hydrochloride could improve fish meat quality, it participate body lipid metabolism by increasing methylic products of fish liver and muscle.

实验结果提示:甜菜碱可改善肉质,它参与机体代谢活动,使肝脏和肌肉中甲基化产物含量增加,影响肠系膜脂肪酶活力,改变体脂和鱼肉脂肪酸组成,进行体脂重分配。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体;用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠,用血凝抑制实验和酶联免疫吸附试验对免后2~13周的小鼠血清的抗体水平进行检测,HI检测其特异性抗体,ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后,碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性;成脂诱导后油红染色呈阳性,有一定数量的脂滴形成。

It indicate that 200μmol/m2s and 80μmol/m2s low light cause reactive oxygen species quick producing and SOD and CAT acticity enhance, but the latter's increasing cann't offset reactive oxygen species'quick producing, which lead to membrance peroxidize injury on peach tree. 30μmol/m2s low light's injury on peach tree mainly because reactive oxygen species'accumulation.

表明200μmol/m2s和80μmol/m2s的弱光下桃树抗氧化酶活性的诱导提高不足以抵消弱光下自由基的快速生成,从而导致对桃树细胞膜脂的伤害;30μmol/m2s的弱光下桃树活性氧的快速生成加剧膜脂过氧化程度,是造成弱光对桃树伤害的重要原因之一。

The proportion of GGT complexed with low density lipoproteins plus very-low density lipoproteins in total GGT in serum is determinated with ratio colorimetery,sodium phosphotungstate and MgCl2 as precipitation.26 patients with liver cancer,18 with liver cirrhosis,22 with chronic hepatitis,14 with the other malignant tumors,16 with non-hepatobiliary disease of high GGT and 50 healthy subjects have been evaluated.

本文以磷钨酸-氯化镁作沉淀剂,速率法测定血清低密度脂蛋白,极低密度脂蛋白结合的r-谷氨酰转移酶(LDL-VLDL-GGT)占总GGT的百分比。对临床26例肝癌、18例肝硬化、22例慢性肝炎、14例其他恶性肿瘤、16例GGT升高的非肝脏疾病及50例健康人进行了检测,结果表明:肝癌组明显高于其他各组,差异显著(P<0.01)。肝脏疾病升高幅度:肝癌>肝硬化>慢性肝炎,且每两者间有显著差异(P<0.01)。

rubia cordifolia l can promote the mice capacity of antioxidation and reduce the content of mda, have obvious improve great mouse's sports ability.

补充茜草提取物可显著提高小鼠心肌和肝脏组织抗氧化酶的活性,减轻大强度耐力训练对大鼠心肌和肝脏组织造成的脂质过氧化损伤,具有明显的抵抗脂质过氧化的功能,对小鼠运动能力的提高有良好的作用。

Systematic aerobic exercise upregulates expression of enzyme genes concerning tricarboxylic acid cycle, and downregulates expression of genes concerning muscle protein synthesis and sphingolipid. It is suggested that aerobic exercise is good to protect human nerves' integrity, exerts positive action on anti-aging and accelerate the aerobic metabolism of lipid materials in vivo.

有氧运动可使三羧酸循环相关酶基因表达上调,肌肉蛋白合成相关基因和神经鞘脂类相关基因表达下调,提示有氧运动有助于保护神经细胞的完整性,对抗衰老有积极作用,同时可加速体内脂类物质有氧代谢。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。