脂酶

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法，以不同浓度亚硒酸钠及半乳糖作用于晶状体，诱发大鼠晶状体形成白内障，在培养初期测定晶状体内非蛋白质巯基（nonprotein sulfhydryl， NP-SH、蛋白质巯基（protein sulfhydryl ，P-SH）和不溶性蛋白质二硫键的含量，脂类过氧化水平，以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase， GSH-PX)、谷胱甘肽还原酶(GSH reductase， GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase， GSH-S)的活性。

Using the middle cerebral artery occlusion modelin rats and cerebral ischemia-reperfusion models in gerbils and mice, we investigated the influence of pueraria compound on the brain water content and the infarct size, the cerebral apoplexy exponent, the contents of lactic acidand lipid peroxide, the activities of lactic dehydrogenase, glutathione peroxidaseand Na+-K+-ATPase.

采用大鼠大脑中动脉栓塞模型、沙土鼠全脑缺血再灌模型和小鼠脑缺血再灌3种动物模型，研究复方葛根素对脑缺血再灌注脑含水量和梗塞范围、脑卒中指数和脑组织中乳酸、乳酸脱氢酶、过氧化脂质、谷胱甘肽过氧化物酶、Na+-K+-ATP酶的影响。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

Aegle marmelos ; Anti-lipid peroxidation ; 4-Methoxy-1-methyl-2-quinolone; Superoxide dismutase; Catalase ; GSH-peroxi

木桔；抗脂质过氧化作用； 4－甲氧基－1－甲基－2－喹诺酮；超氧化物岐化酶；过氧化氢酶；谷胱甘肽过氧化物酶

Diacylglycerol acyltransferase 1(DGAT1) is a microsomal enzyme that catalyses the final and only committed step in the formation of triglycerides.

二酰基甘油酰基转移酶1(Diacylglycerol acyltransferase，DGAT1)是催化酰基化的辅酶A和二酰基甘油合成甘油三脂的关键限速酶。

The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase, and not a lipase.

底物特异性实验确定DF-B6菌株分泌的脂裂解酶为酯酶，而非脂肪酶。

MDA content, cell division , he activities of NR, CAT, POD and SOD of Pistia sagittifolia and Myriophyllum Spicatum with heavy metal,s ion concentration to appear a trend of ascending firstly and desending secondly . This trend explains that Pistia sagittifolia and Myriophyllum Spicatum on the Toxic Effects have similarities under handing the single Pb2+ and Hg2+. As the range of the concentration which each index is arriving at the maximum is different, this also indicates that there are some differences of the Toxic Effects between Pistia sagittifolia and Myriophyllum Spicatum under handing the single Pb2+ and Hg2

丙二醛含量、脂膜透性、硝酸还原酶活性、过氧化物酶活性、超氧化物歧化酶活性、过氧化氢酶活性随重金属离子浓度的增加呈先升后降趋势，说明在单一Pb2+和Hg2+处理下大薸和穗状狐尾藻毒害效应具有相似性；但各指标到达最大值的浓度范围不同，又说明单一Pb2+和Hg2+处理下大薸和穗状狐尾藻毒害效应具有差异性。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。