脂质体的

Objective To prepare vinorelbine bitartrate liposome and compare the difference of the entrapment efficiency by using Sephadex minicolumn centrifugation method and cation exchange resin centrifugation method, respectively.

目的 制备重酒石酸长春瑞滨脂质体，比较葡聚糖凝胶微柱离心法和阳离子交换树脂离心法测定重酒石酸长春瑞滨脂质体包封率的差异。

Brucine and its liposome could inhibit the growth of transplanted solid tumor and the inhibitory effect of its liposome is much stronger than that of brucine.

马钱子碱及其脂质体有显著的抑制荷瘤小鼠肿瘤生长作用，马钱子碱脂质体抗肿瘤作用明显强于马钱子碱。

This paper aimed at studying the preparation process of MCFA liposome and evaluate its quality.

本实验旨在研究中链脂肪酸脂质体悬浮液和冻干制剂的制备，并对脂质体进行质量评价。

Methods: Lecithin and the sucrose ester-phospholipids liposome were prepared using the ethyl ether injection method by mixing sucrose ester with lecithin, sphingomyelin and cholesterol according to different lipids ratio.

按不同比例将乙酸异丁酸蔗糖酯与卵磷脂、鞘磷脂和胆固醇混合，溶于乙醚中，然后注入pH7.2的PBS缓冲液中，制成卵磷脂脂质体及蔗糖酯－卵磷脂脂质体。

Liaoning Medical University, Jinzhou 121001 China）Abstract:Objective To study the distribution and liver targeting of glycyrrhetinic acid derivatives (stearin glycyrrhetinic acid ester-3-O-galactosidase, Gal-GAOSt) modifying norcantharidin liposomes (Gal-GAOStNC-LP) in rats.

目的 研究甘草次酸衍生物（甘草次酸硬脂醇酯-3-O-半乳糖苷，Gal-GAOSt）修饰去甲斑蝥素脂质体(Gal-GAOStNC-LP)在小鼠体内的分布及肝靶向性。方法利用薄膜分散法制备甘草次酸衍生物修饰去甲斑蝥素脂质体后，经小鼠尾静脉给药，采用HPLC法检测去甲斑

Aim:To observe the therapeutic effects of liposomeencapsulated adriamycinadministered into the hepatic artery for hepatoma in rats fand compare it with that of adriamycin solution and ADM plus blank liposome.

目的：观察阿霉素脂质体经肝动脉给药对大鼠肝肿瘤的疗效，并与阿霉素水溶液及阿霉素加空白脂质体相比较。

Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers.

电镜观察表明，该脂质体多呈球形的单层或多层结构，脂质体粒径分布均匀，粒径范围20-700 nm，平均粒径163 nm，主要粒径分布位于50-200 nm(84.80%)。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Methods BALB/c nude mice were subcutaneously transplanted with SCG-7901 tumor cells, then the mice were randomly divided into antisense oligonucleotide group, sense oligonucleotide group and the control group. The HIF-1α ASOND complexed with cationic liposome, HIF-1α SOND complexed with cationic liposome and liposome were injected intra-tumorally in the above groups, respectively.

用人胃癌细胞株SCG-7901建立胃癌裸鼠皮下移植瘤模型，将荷瘤裸鼠随机分为反义寡核苷酸组、正义寡核苷酸组和对照组，瘤体内分别注射阳离子脂质体包裹的HIF-1αASOND、HIF-1αSOND和空白阳离子脂质体。

In present study, we observed effect of taurine on liver lipid metabolism in rat, an animal capable of synthesizing substantial amount of taurine and extensively used in studies on lipid metabolism, and tried to explain its biochemical basis by investigating several hepatic microsomal membrane-bound enzymes activities related to lipid metabolism.

本实验中，我们将观察牛磺酸对大鼠肝脂质代谢的影响，并观察牛磺酸对大鼠肝脏微粒体脂质代谢酶活力的影响，以探讨牛磺酸影响肝脏脂质代谢的可能机制。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。