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The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Membrane affinity quantified by IAM and liposome/buffer systems was a more efficient predicator of α values than hydrophobicity from n-octanol/buffer system. Consequently, not only hydrophobic force but also attractive polar extra-interactions involved in the binding to IAM and liposomal membranes but not in an n-octanol phase, ascribed to the binding process within AM. The binding to ordered lipid membrane in the larger part determined drugs'binding to AM intracellular compositions, and the ordered phospholipid plasma membrane was a potential site for drugs'binding in AM.

脂质体/水系统和磷脂膜色谱所测定的膜亲和性比正辛醇/水系统的疏水性更好的预测巨噬细胞内药物的α值,因此包含在磷脂膜色谱中但并不体现在正辛醇/水系统中药物与磷脂膜之间的额外吸引性极性作用力对药物在细胞内结合有着重要贡献;药物与有序磷脂膜的结合在很大程度上决定着药物与巨噬细胞内成分的结合,有序磷脂分子膜可能是巨噬细胞内部潜在的结合部位。

Furthermore, taurine-treated microsomes showed a higher incorporation of radiolabled oleoyl-CoA into diacylglycerol, phosphatidylethanolamine, sphingomyelin and lysophosphatidylcholine lipid fraction in comparison with the control microsomes.

牛磺酸可改变微粒体膜脂质成分(如降低游离胆固醇、磷脂酰丝氨酸和鞘磷酸,升高磷脂酰乙醇胺和磷脂酸的浓度)和膜磷脂脂肪酸成份,并降低微粒体膜脂质的流动性。

It was suggested that the pharmacodynamics and therapeutic function of Qizhi complex prescription to fatty liver were: 1. Adjust lipometabolism: reducing TC and TG; 2. Decreasing fatty degeneration of liver cell; 3. Promoting the convalescence of hepatocellular function; 4. Anti-lipid peroxide: increasing the activation of peroxidase, decreasing the content of products of lipid peroxide. 5. Restraining Kupffer cell function of fatty liver rat; 6. Improving fluidity of hepatocellular microsome and mitochondria.

芪枳合剂治疗脂肪肝的效应及药效学机制为: 1、调整脂质代谢:降低血清甘油三脂和胆固醇; 2、减轻肝细胞脂肪变性; 3、促进肝细胞功能恢复,有效治疗脂肪性肝炎; 4、抗脂质过氧化:提高抗氧化酶活性、降低脂质过氧化产物含量; 5、抑制脂肪肝大鼠Kupffer细胞功能; 6、改善肝细胞微粒体、线粒体膜流动性。

Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象,在对〓细胞抑癌基因(Rb基因、p53基因)表达状况进行检测和鉴定的基础上,通过建立裸鼠玻璃体腔RB常位异种移植模型,构建Rb基因的逆转录病毒表达载体,以脂质体介导的Rb基因转移方式,进行RB体内基因治疗的实验研究,观察Rb基因在体内对RB的治疗作用及影响因素,并探讨其作用机制。

Eukaryon expression vectors containing humanbcl-2 and bax cDNA were respectively transfected into HCC-9204 cells,HCC cellsthat express Bax stably and highly were established,and an HCC cell strain thatexpress Bcl-2 at a 100% positive rate was obtained by cloning.

用脂质体介导的基因转染法分别将含有人bcl-2和bax cDNA的真核表达载体转染到HCC-9204细胞中,建立稳定高表达Bax的肝癌细胞,并用克隆化的方法建立100%稳定表达Bcl-2的肝癌细胞株。

In second-line drugs for the treatment of recurrent epithelial ovarian cancer,liposomal doxorubicin is more effective and well tolerated.

脂质体阿霉素在复发性卵巢上皮性癌二线化疗药物中有较高的疗效优势,且具有更好的安全性和可耐受性,楷莱可作为复发性卵巢上皮性癌非铂类化疗的理想选择。

V increased (other than Ara-C) and the inhibitory effect against 〓 improved significantly, especially for ADM. The empty pro-liposomes may undergo three process when hydrated with drug solution: swelling of phospholipid film, fracture of film and resealing to form vesicles.

以空白脂质体前体为载体,对几种临床上常用的抗癌药物的抑瘤活性及毒性进行了研究,研究结果得出:与游离药物相比,5-Fu,阿霉素等药物的抑瘤活性都有不同程度的提高;急性毒性实验表明:除Ara-C外,几种药物的毒性均有所降低,以顺铂最明显。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞,实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%),稳定转染得到的阳性克隆最多,且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少(约20d);用阳离子聚合物瞬时转染率低(1.7%),稳定筛选同样可以得到阳性大克隆,但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多(约30d);阳离子脂质体转染效果居中,瞬时转染率为39.9%,从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。