脂质体的

Main effect: The specific pearl essence, L-Ascorbic acid and VE liposome can obtain the effect of enduring moisture retention and nourishing white skin at the same time of whitening skin.

主要作用：特含的珍珠精华、左旋VC和VE脂质体在美白的同时，可达到持久保湿、养白肌肤的效果。

An exploration of applications of sugar-based surfactants in the solubilization of liposomes and cell membranes rounds out the coverage.

探索应用糖基表面活性剂的增溶的脂质体和细胞膜轮的覆盖面。

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

Methods: 1. Leukemia cell lines HL-60、Jurkat、Molt-4 were grown in RPMI medium 1640 supplement with 10% fetal calf serum and 100U/ml benzylpenicillin and 100 U/ml streptomycin sulfate in a humidified atmosphere containing 5% of CO2 at 37oC.

用脂质体将测序正确的重组子转染至白血病细胞株HL-60、Jurkat、Molt-4中，用RT-PCR、Western Blot方法检测iASPP的表达以及用流式细胞仪检测细胞凋亡的变化状况。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

With fluorescin as reporter molecules, the distribution and elimination of the liposome were tested in vivo in mice.

导向脂质体可以明显减慢荧光素钠在小鼠体内的吸收与消除的过程，并明显提高荧光素钠在肝组织中的分布含量。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

SDS could dissolve the protein envelop as well as the lipide membrane of pathogen and cell. SDS is applied in light industry widely and safely, SDS is also made as microbicides in topical vaginal formulations to prevent the transmission of herpes and possibly other pathogens that cause sexually transmitted diseases,including HSV.

十二烷基硫酸钠(Sodium dodecyl sulfate，SDS)是一种阴离子型表面活性剂，为生物膜的最有效的溶解剂，它可以同病菌和各种高等动物的细胞膜的磷脂等脂质体结合而溶解，与蛋白质结合而变性。

Results In Vero cell system,in direct virucidal and infection block experiments,fullerene C60 liposomes have induction effect on the expression of HSV-1F strain glycoprotein gC,whether it is under illumination or in lucifugal conditions,but in multiplication inhibition experiments,fullerene C60 liposomes have no effect to the expression of HSV-1F strain gC.

结果 在Vero细胞系统中，C60脂质体在直接杀灭和感染阻断的实验中，不论在光照还是在避光条件下，对HSV-1F株gC的表达均有诱导作用；但是在增殖抑制实验中，对HSV-1F株gC的表达没有影响。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。