脂质体的

ResultsThe transfection efficiency was 67%, 43% and 28% by lipofectamine, electroporatin and calcium acid phosphate.

结果脂质体法的转染率为67%，电穿孔的转染率为43%，磷酸钙法的转染率为28%。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

However, high temperature and humidity are not suitable for storage.

脂质体包裹的NGF是稳定的，较高的温度和湿度不利於储存。

First, we designed to determine the optimum ultrasound-setting, such as power level and ultrasound frequency, to achieve the optimum number of ruptures in the spinotrapezius muscles by intravital microscopy.

我们设计了应用活体荧光显微镜，测量不同条件超声介导微泡破坏作用后鼠脊斜方肌血管破裂口的数量和荧光脂质体微泡的弥散面积，确定最佳超声发射参数；然后，在慢性鼠脊斜方肌模型上，测量荧光标记蛋白的荧光强度和血管密度，对比两种血管内VEGF基因载体系统经超声介导微泡破坏作用后定向转基因表达的效果。

In this contribution we have showed some aspects of preparation biocompatible magnetizable complex systems (nanoparticles, magnetic fluids and magnetoliposomes), their functionalization for application in biomedicine and some medical application.

在本文中，我们介绍几种生物相容性的磁性复合体系统（纳米颗粒，磁性液体和磁性脂质体）的制备，以及为实现其在生物医学和其它医学领域应用进行的功能化加工。

We firstly demonstrated that the effectiveness of gene transfer by SA liposomes in vivo.

我们首次证实SA脂质体介导的体内基因转染是有效的。

In gene expression and regulation studies, Cationic liposomes have been commonly used to delivery functional genes into cells.

在基因表达调控研究中，DNA转染是常见的技术手段，其中脂质体转染法是将外源基因导入培养细胞和组织的有效方法。

There is no obvious difference of the secondary structure between BR monomers and trimers.

BR单体与三聚体的性质差异是由于其周围脂质环境的物理状态变化，而不是蛋白结构发生了变化。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。