脂质体的

In the third part,the effects of liposomal transfection of ASON on alpha-globin in beta-thalassemia major erythroid cells cultured in vitro were observed.

第三部分，观察了脂质体转染高效ASON对体外培养的重型β-地中海贫血红系细胞α-珠蛋白的调控作用。

Phosphatidylcholine is a kind of phospholipid, which has special character and physiological function. It widely used in food, cosmetic, and pharmaceutical industries. In particular, it is promising to prepare liposomes and pharmic emulsifier.

磷脂酰胆碱，是一种磷脂，具有特殊的理化性质和生理功效，广泛应用于食品、医药、化妆品等领域，尤其在作医药乳化剂和脂质体制备方面具有广阔的前景。

A little of the diaylsed solution was dilluted with distilled water (1:4) and applied to metallic sample plate following negative staining with sodium phosphotungstate solution. The sample was freeze-dried and examined by transmission electron microscopy 1.4 IFvs size and size distribution measured 1.5 The shape morphology of IFvs 1.6 Measureing the encapsulation efficiency and loading capacity of Liposomal Gd-DTPA A sample volume of 5ml was sent to analyze (Zhongshan University Analytical Center, Guangzhou).

1.6 Gd-DTPA脂质体包封率及载药量的测定取5ml前述离心后所得上清液送交中山大学分析测试中心，采用高频电感耦合等离子发射光谱法测定离心液中Gd-DTPA含量，用加入总的Gd-DTPA量减去离心液中的Gd-DTPA含量即得包封量。

The recombinant pIND-ndr2vector and pIND vector were tranfected into GLC-82cells respectively,and the cell clones were screened with G418and zeocin.

连有ndr2基因的载体（pIND-ndr2）用脂质体方法转染到培养的肺腺癌细胞GLC-82中。

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

To monitor transfection efficiency, 50 ng of the pRL-null plasmidencoding Renilla luciferase was included in all samples.

HEK293细胞接种到12孔板和0.5微克的神星启动建设用脂质体转染 2000（Invitrogen公司）根据制造商的说明。

However, the activity decreased for copper salicylate encapsulated in positively and negatively charged liposomes.

但正负电性脂质体包裹的水杨酸铜的SOD样活性降低。

Liposomal entrapment efficiency of urokinasein by MREV is as high as 65％, which is not evidently influenced by incorporation of phospholipid derivatives such as DSPE-PEG-RGDS and Stearyl RGDS.

改良反相蒸发法在磷脂相转变温度以下制备脂质体以提高包封率的效应在尿激酶上得到了很好的体现。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

The release property of small unilamellar vesicles coated with the copolymer was studied at 25℃ by using 5(6) carboxyfluorescein 5(6 CF as a water soluble marker.

以5（6）－羧基荧光素（5（6）－CF）为水溶性标记物，研究了共聚物包覆的小单层脂质体（smalunilamelarvesicles，SUV）在室温25℃时的释放行为。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。