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Objective: To observe the effects of the liposome composed by sucrose ester and phospholipids on the membrane phosphatide content of rabbit peritoneal macrophages.

目的:观察蔗糖酯与磷脂组成的脂质体对巨噬细胞膜磷脂含量变化的影响。

Methods: cationic liposome lipofectamine2000 was used to transfect retrovirus vector pbabe pten puro containing pten and pbabe puro into pten deficient prostate cancer cell line pc 3 respectively, and then cell growth, adhesion, segregation, apoptosis, cell cycle and ultrastructure alteration in vitro were studied.

利用阳离子脂质体lipofectamine 2000介导,分别将含pten的逆转录病毒载体pbabe pten puro及空载体pbabe puro转入pc 3细胞,观察转染前后细胞的生长、粘附、超微结构、细胞周期改变情况。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别,采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞,经7天G418(600μg/ml)药物筛选,共获得30个neo阳性细胞克隆,其中12个为非绿色细胞克隆,仅有7个可以扩大培养。

Methods: The NGF purified from mouse submaxillary glands was packed with liposome and prepared into freeze dying agent.

将纯化得到的小鼠颌下腺的NGF用脂质体包裹后分别制成冻干剂型。

OBJECTIVE: To prepare cholesterol succinyl chitosan anchored liposomes, and to investigate the effect of CHCS anchored liposomes on the release property in vitro of loading drugs taking epirubicin as a model drug.

目的:通过"锚定"的方式制备胆甾醇琥珀酰基壳聚糖锚定脂质体,并以表阿霉素作为模型药物,考察其对包载药物体外释放性质的影响。

Abstract] Objective: To investigate the effect of the mycobacteriophage D29 liposome-coating liquid in the mice infected by multi-drug resistant tubercle bacillus, as a new method in treating multi-drug resistant tuberculosis.

[摘要]目的:研究D29分枝杆菌噬菌体脂质体包被液对耐多药结核杆菌感染小鼠的治疗效果,探寻治疗耐多药结核病的新方法。

Mycobacteriophage D29 liposome-coating liquid has a beneficial effect in mice infected by multi-drug resistant tubercle bacillus, which may be a new method in treating multi-drug resistant tuberculosis.

噬菌体脂质体包被液对耐药结核菌感染小鼠具有良好的治疗效果,有可能成为治疗耐多药结核病的新方法。

OBJECTIVE To study the effect of the lipid compositions on cellular uptake and gene transfection mediated by cationic liposomes.

目的研究阳离子脂质体膜的组分对细胞摄取和基因转染的影响。

The full-leng coding sequence of ALK1 was cloned into pcDNA3.l+using standard protocols. The constructed pcDNA3.1+ALK1 plasmid were transfected into HUVECs. The proliferation and migration of HUVECs were detected by boyden champer and flow cytometry.

脂质体转染pcDNA3.1+ALK1到HUVECs,流式细胞仪检测HUVECs增殖的改变,boyden小室检测ALK1对HUVECs迁徙的影响。

A useful and efficient glutaraldehyde method for covalent binding of antibodies to lipo-somes was discussed.

本文报道了一种实用、高效的脂质体与抗体共价偶联的方法—戊二醛法。

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The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为:以确有疗效的中药复方为研究对象,以现代科学技术和传统中医药理论为技术支持,以该复方所治病证的药效响应为评价标准,以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。

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