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The fatty acid components of plant lipids come mainly from acetyl CoA, produced in plastids and mitochondria.

植物脂质中的脂肪酸组分主要来源于乙酰辅酶 A ,在质体和线粒体中生成。

The methods of purification and reconstitution of Ca〓-Mg〓-ATPase were set up and Ca〓-Mg〓-ATPase from both spontaneously hypertensive rat and control rat were isolated, purified and reconstituted in the study.

建立了质膜钙泵的分离纯化方法和磷脂重建方法并用这些方法成功地分离纯化了正常大鼠和自发性高血压大鼠红细胞膜钙泵,而且将其重建于人工脂质体上。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的:探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

By the electropositive Liposome 2000, in the 6-plate for cell culture, pEGFP-P80 above is transfected into the PK-15 cells from the kidney of pigs and expressed.

利用阳性脂质体L2000 介导,在六孔细胞培养板中,将已经构建好的pEGFP-P80重组表达质粒转染到PK-15 细胞中进行表达。

Expression of M, NP, F and HN genes were detected and confirmed by the indirect immunofluorescence analysis. The syncytium formation was observed in the HeLa cells co-transfected with pCAGG-M, pCAGG-NP, pCAGG-F and pCAGG-HN recombinant plasmids at 48h post transfection.

纯化后的重组质粒通过脂质体单独转染HeLa细胞,经间接免疫荧光试验检测到了M、NP、F和HN蛋白的表达。pCAGG-M、 pCAGG-NP、pCAGG-F和pCAGG-HN重组质粒组合共转染HeLa细胞48h后,可以观察到明显的细胞融合现象。

Methods The drug release profiles of liposome encapsulated psoralen were evaluated by dialyzation using psoralen gel as the control.

摘 要 目的制备治疗白癜风的补骨脂素脂质体凝胶,对其体外释药模式进行定量考察。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞,探讨不同基因对骨髓基质干细胞成骨活性的影响,我们首先利用RT-PCR方法获得了全长BM P-2,VEGF 165及bFGF基因,克隆入真核表达载体pcDNA 3.0,鉴定正确后,经脂质体介导转入分离培养的大鼠骨髓基质干细胞,G 418筛选出稳定表达株。

Comparison from the Germany isolate, phocine distemper virus type Ⅱ(PDV-2) and vaccine strain, the major difference is the long signal peptide domain while the mature protein exhibit high identity, and all of the 13 serine residues, four potential asparagine glycosylation sites and two hydrophobic regions supposed to affect the fusion function are completely identical. These data supports the view that F protein is more conservative than H protein in CDV.

三、犬瘟热病毒中国分离株囊膜糖蛋白基因免疫小鼠诱发的抗体应答经一系列步骤将CDV-YZ0101株的两囊膜糖蛋白基因定向导入真核表达载体pcDNA3.1中,DNA测序和酶切分析筛选阳性重组质粒克隆pcDNA-H和pcDNA-F,以重组质粒DNA和脂质体共转染COS-7细胞,用间接免疫荧光试验证实转染的COS-7细胞的胞浆中分别表达了CDV的H和F蛋白。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞,以未转染细胞作阴性对照。

Addition of DSPE-PEG-RGDS and Stearyl RGDS in liposomal prescription gives birth to two targetedly thrombolytic liposomes. The former has long circulating function, the latter has no long circularing action but carries two times directing molecules as that carried by the former.

将DSPE-PEG-RGDS和Stearyl RGDS分别掺入脂膜中制备得到了两种靶向溶栓脂质体,前者同时具有长循环作用;后者不具长循环功能,但导向分子的携载量为后者的两倍。

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The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为:以确有疗效的中药复方为研究对象,以现代科学技术和传统中医药理论为技术支持,以该复方所治病证的药效响应为评价标准,以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。

I don't… don't know. He's unconscious.

我不……我不知道他休克了。